Habilitationsschrift--Hamburg. / In German with summary in English and German. Includes bibliographical references (p. 235-264) and index.

Habilitationsschrift--Hamburg. / In German with summary in English and German. Includes bibliographical references (p. 235-264) and index.

Thesis (M.S.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains vi, 52 p. : ill. Vita. Includes abstract. Includes bibliographical references (p. 37-38).

Honors Project--Smith College, Northampton, Mass., 2010. / Includes bibliographical references (p. 77-80).

Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.

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FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / Angiogenesis is the development of new blood vessels from pre-existing capillaries, being an essential step in tumor growth for supplying nutrition and oxygen to cells in proliferation. A cell that may be involved in this process is the mast cell (MC), since besides the defense function, acts in the blood vessels regulation. The MC participation in the induction of angiogenesis has been suggested in various malignant tumors. The purposes of this study was to evaluate angiogenesis and mast cell density in oral epithelial dysplasia and squamous cell carcinoma (SCC). This is an observational, retrospective and quantitative study using the sample selection from the archives of the Department of Legal Medicine and Pathology and Laboratory of Oral Pathology, both from the Federal University of CearÃ. For MC evaluation , the sample was consisted of 73 paraffin blocks, distributed between SCC (n=30), epithelial dysplasia (n=23) and hyperplasias fibroepithelial (HFE) (n = 20), as control, and for angiogenesis the sample was 65 blocks, consisted of 24 SCC, 19 epithelial dysplasias and 22 HFE. Immunohistochemistry was performed using the MC-tryptase, CD31 and CD34 antibodies. For quantification, digital images were captured and then counting was performed using Image J software. The antibody staining percentage was determined using SAMM software. With regard to mast cells, there was a lower density in malignant lesions in relation to HFE and dysplasia (p = 0.0092). Evaluating angiogenesis, CD31 expression showed differences between epithelial dysplasia and SCC and between SCC and HFE, with a greater percentage of vessels in SCC (p <0.0001). However, CD34 expression did not differ between groups. The CD31 antibody was shown to be a better angiogenesis marker in oral mucosa than CD34. Increased vascularity in oral squamous cell carcinoma suggests that angiogenesis is necessary for tumor growth, increasing when the malignant transformation starts. However, no correlation was found between mast cells and angiogenesis. / AngiogÃnese à o surgimento de um novo vaso sanguÃneo a partir de capilares prÃ-existentes, sendo um passo essencial no crescimento tumoral por fornecer nutriÃÃo e oxigÃnio Ãs cÃlulas em proliferaÃÃo. Uma cÃlula que pode estar envolvida nesse processo à o mastÃcito, pois, alÃm da funÃÃo de defesa, atua na regulaÃÃo de vasos sanguÃneos. Sua participaÃÃo na induÃÃo da angiogÃnese tem sido sugerida em vÃrios tumores malignos. Os objetivos deste trabalho foram avaliar a angiogÃnese e a densidade de mastÃcitos em displasias epiteliais e no carcinoma espinocelular (CEC) de boca. Trata-se de um estudo observacional, retrospectivo e quantitativo, realizado atravÃs da seleÃÃo de amostra proveniente dos arquivos do Departamento de Patologia e Medicina Legal e do laboratÃrio de Patologia Bucal do curso de Odontologia, ambos da Universidade Federal do CearÃ. Para a avaliaÃÃo dos mastÃcitos, a amostra foi constituÃda por 73 blocos parafinados, distribuÃdos entre CEC (n=30), displasias epiteliais (n=23) e hiperplasias fibroepiteliais (HFE) (n=20), como controle, e para a angiogÃnese a amostra foi de 65 blocos, sendo 24 de CEC, 19 de displasias epiteliais e 22 de HFE. Foi realizada imunohistoquÃmica utilizando-se os anticorpos anti-triptase, para mastÃcitos e anti-CD31 e anti-CD34, para vasos sanguÃneos. Para quantificaÃÃo, foram capturadas imagens digitais e, em seguida, utilizados softwares para auxiliar na contagem dos mastÃcitos (Image J) e para determinaÃÃo do percentual de marcaÃÃo do anticorpo (SAMM). Com relaÃÃo aos mastÃcitos, houve menor densidade destes nas lesÃes malignas em relaÃÃo Ãs HFE e displasias (p=0,0092). Avaliando angiogÃnese, a expressÃo de CD31 mostrou diferenÃa entre os grupos CEC e displasia epitelial e entre CEC e HFE, havendo um maior percentual de vasos nos CEC (p<0,0001). Contudo, o CD34, nÃo mostrou diferenÃa entre os grupos. O anticorpo CD31 mostrou-se melhor marcador de angiogÃnese em mucosa oral do que CD34. O aumento da vascularizaÃÃo em CEC oral sugere que a angiogÃnese à necessÃria ao crescimento tumoral, aumentando à medida que inicia o processo de malignizaÃÃo. NÃo foi encontrada correlaÃÃo entre mastÃcitos e angiogÃnese.

Betel quid¡]BQ¡^chewing is recognized as a major risk factor for oral precancerous lesions¡]OPLs¡^in Taiwan. The compositions of Betel quid could cause DNA damage. X-ray repair cross complementing Group 1¡]XRCC1¡^plays a crucial role in the process of DNA repair. Polymorphisms in XRCC1 gene may affect DNA repairing ability and modulate the susceptibility of OPLs. The aim of this study was to investigate the association of XRCC1 genetic variants with the risk of BQ-related oral precancerous lesions, including oral leukoplakia¡]OL¡^ and oral submucous fibrosis ( OSF ). A total of 449 males¡]169 OL cases, 82 OSF cases, and 208 healthy controls¡^who habitually chewed BQ were recruited. The genotypes were determined by PCR-RFLP and TaqMan real-time assays. The C allele and T/C+C/C genotypes at XRCC1 -77 were associated with the reduced risk of OL ( AOR=0.54, 95%CI:0.34-0.85 and AOR=0.47, 95%CI:0.28-0.78, respectively ). The 399Gln allele and 399 Arg/Gln+Gln/Gln genotypes were associated with the increased risk of OL¡]AOR=1.94; 95%CI: 1.41-2.67 and AOR=2.64; 95%CI: 1.73-4.03, respectively¡^and OSF ( AOR=1.67; 95%CI: 1.11-2.49 and AOR=2.30; 95%CI: 1.35-3.91, respectively ). The haplotypes or diplotypes contain fewer risk alleles¡]-77T or 399Gln¡^ were with lower risk of OL¡]both Ptrend<0.001¡^and OSF (Ptrend=0.056 and Ptrend=0.040, respectively). In conclusion, our results suggest that polymorphisms of XRCC1 at -77 and 399 may be associated with the risk of OPLs.

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