This thesis is dedicated to theoretical solutions and practical application of valuation methods used for evaluation of young startup companies. The aim of this work is to provide readers with theoretical introduction into the startup scene, conceivable valuation approaches and their practical application on the startup followed by drawing conclusions and recommendations. The thesis is divided into theoretical and practical part. The first part deals with the theoretical foundations of startups, the analysis of the Czech startup scene and possible arguments for valuing startups. The thesis discusses the specific characteristics of startups and their impact on the valuation process. The theoretical part is concluded by an analysis of the theoretical methods for the startup value estimation. In the practical section, selected valuation methods are applied on a particular startup company operating in the Czech Republic. Finally, the practical part summarizes the problems and specifics that arise during the valuation process followed by recommendations for valuation practice.