The thesis deals with molecular detection of resistance in populations of pollen beetle based on genetic polymorphisms in the VSSC gene of this pest. Population samples were collected in 2016-2018 in the Czech Republic, mainly in the Southwestern Bohemian Region. A total of 57 populations of pollen beetles with different degrees of resistance were analyzed. Nucleotide mutations of populations were monitored in two parts of the VSSC gene of pollen beetles - DIIS4 - S5 linker / DIIS5 and DIIIS6 gene, which is located in the VSSC binding domain. These parts were amplified and sequenced by the Sanger method. In the alignment of final sequences, mutations were searched. After that, the effect of these mutations on the amino acid sequence was determined and the effect on the resulting VSSC protein conformation was assessed.

This thesis deals with the effectiveness of insecticides on Colorado potato beetle, which is one of the most serious pests that damages the above-ground part of the potato crop. Field trials were established in the years 2015-2017 at the campus of the Mendel University School in Žabčice. Insecticides from the group of neonicotinoids (Actara 25 WG, Biscaya 240 OD, Mospilan 20 SP and Monceren G) were used here. Insecticides from the group of pyrethroids (Vaztak Active, Nurelle D, Karate se Zeon 5 SC) and diamides (Coragen 20 SC) were used. Last but not least, two insecticides (NeemAzal T / S, SpinTor) have been applied that are useful in organic farming. In 2015 and 2017, the presence of Colorado potato beetle was evaluated before and after application of individual insecticides. Based on the occurrence of potato beetle larvae, efficiency was calculated. In addition, the difference between insecticides and insecticides and control was statistically evaluated. The year 2016 was not evaluated because of the low number of potato beetles.

The aim of this thesis was to determine presence of resistant populations pollen beetle (Brassicogethes aeneus). Determination their resistance level and proposing an anti-resistant strategy was solved too. The adults of pollen beetle were collected in year 2012 and 2013 from 16 locations in South Moravia. Collections were done on rape, but also on poppy and mustard. Pyrethroids - cypermethrin, lambda-cyhalotrin, tau-fluvalinate; neonicotinoids -- commercially used preparation Biscaya 240 OD and organophosphorous insecticide active substance chlorpyrifos ethyl were tested. The tests were conducted according to the methodology of Insecticide Resistance Action Committee (IRAC) number 011, 021, 025. Each active substance was tested in three repetitions. The adults were exposed to 100 % registered dose after 24 hour. Substance efficiency in 2012 and 2013 was different. There was an efficiency drop in Biscaya 240 OD preparation. On the contrary, lambda-cyhalotrine substance was more effective in 2013.