Purpose: To examine the relationship between visual acuity (VA) and radial deformation acuity (RDA) in children 6 to 12 years of age with amblyopia. Methods: RDA was measured in 35 participants with the Manchester RDA charts. VA was measured with the Early Treatment Diabetic Retinopathy Research Study (ETDRS) chart. Results: Median VA in non-amblyopic and amblyopic eyes was 0.04 logMAR (IQF -0.06 – 0.12) and 0.24 (IQF 0.12 – 0.04), respectively (Wilcoxon Signed Ranks test, z = -5.07, p < 0.001). Median RDA in non-amblyopic and amblyopic eyes was 2.73 log (IQF 2.53 – 2.87) and 2.63 log (IQF 2.53 – 2.77), respectively (Wilcoxon, z = -2.56, p < 0.05). Spearman correlation suggested that the amblyopic deficits in VA and RDA were related, r = -0.42, p < 0.05. Conclusion: A deficit in RDA was present in most children with amblyopia. A moderate relationship was noted between the amblyopic deficits found in VA and RDA.