Global temperature changes are predicted to influence the distributions of plants and can have significant consequences for population genetic structure. Both the nature of these consequences and the processes that shape them are of interest for both conservation genetics and the development of realistic management programs. Rapid range expansion occurs on short temporal scales not conducive to conventional phylogeographical analyses. This paper presents the findings from a population genetic study of the invasive grass Microstegium vimineum Trin. A. Camus throughout the James River Basin of Virginia. Genotypic analysis using Amplified Fragment Length Polymorphism (AFLP) molecular markers were used to test for evidence of rapid range expansion and the effects associated with colonization and spread of Microstegium vimineum. Within the James River Basin three genetically distinct clusters were identified that were not clearly associated with natural geographic boundaries and recent founder events were also inferred. The James River Basin also appears to act as a corridor for long-distance dispersal events. These findings contribute to our knowledge of the genetic consequences of rapid range expansion for invasive species, and more importantly, native species. Contrary to several studies, the present research also indicates that long-distance dispersal is not rare and can be a major contributor to the genetic structure following range expansion.

Introduced species cause both considerable ecological and economic damage every year. However, not much is known about how certain species are able to establish and spread beyond the site of initial introduction, whereas others do not. Species undergoing range expansion following an introduction may prove to be a valuable resource to invasion biology, but may also be informative in light of species' responses to changing environments (i.e. global climate change). Here, I took advantage of an ongoing range expansion of an introduced vertebrate species. House sparrows (Passer domesticus) were introduced to Mombasa, Kenya in the 1950s and have subsequently expanded their range northwest-ward and now occupy most major cities in Kenya. By comparing older, established populations (i.e. those in Mombasa) with more recently colonized populations at the range edge, it might be possible to determine some of the mechanisms that underlie range expansion in some species and/or populations. In Chapter 1, the background and ideas that motivated the rest of the dissertation is summarized. In Chapter 2, I studied how exploration and glucocorticoids (a hormone released in response to stressors) changed throughout the range expansion. Exploration was greater at the range edge, which is likely to ensure greater discovery of novel resources. Glucocorticoids released in response to restraint were also highest at the range edge, which might facilitate resolution of stressors in unpredictable environments. However, chronically elevated levels of glucocorticoids are often considered maladaptive, unless an individual can appropriately cope with them. Therefore, in Chapter 3, I characterized glucocorticoid receptors (i.e. mineralocorticoid receptor (MR) and glucocorticoid receptor (GR)) in the hippocampus, an area responsible for negative feedback of glucocorticoids as well as induction of behavioral and physiological response to stressors. I found that MR density was lower relative to GR density at the range edge compared to the site of introduction (Mombasa). I speculate this pattern is a mechanism to resolve the elevated levels of glucocorticoids at the range edge. Taken together, these results indicate that individuals at the range edge have a strong glucocorticoid response to stressors to induce a rapid, strong response to resolve stressors. Subsequently, in Chapter 4, I examined the potential mechanisms of phenotypic change among Kenyan house sparrows. Typically, following an introduction event, genetic diversity undergoes a bottleneck and is greatly reduced compared to the source population; as such, genetic evolution as the main driver of changing phenotypes throughout the range expansion is unlikely. We therefore hypothesized that epigenetic mechanisms (e.g. DNA methylation) may compensate for the expected reduced genetic diversity following an introduction. Although there was no pattern of epigenetic variation among cities (i.e. variation did not increase nor decrease further from the site of introduction), epigenetic variation increased as genetic inbreeding increased (a sign of reduced genetic diversity and bottlenecks), suggesting epigenetic modifications may compensate for reduced genetic diversity following an introduction event. Overall, patterns of phenotypic variation emerged dependent on age of the population- these patterns may prove to be important in other vertebrate range expansions as well. Surprisingly, epigenetic diversity did not correlate with phenotypic variation among populations; however, within-individual studies may reveal epigenotypes are related to certain behavioral or physiological phenotypes. In the future, studies should be designed to address how phenotypic differences arise despite relatively low genetic diversity and overall high genetic admixture among individuals. In Kenyan house sparrows, maintenance of high levels of flexibility and differential developmental influences may be important factors that lead to varying phenotypes dependent on time since colonization.

No description available.

Invasive species are problematic but their control can be difficult. When the history and dynamics of invasive populations are unknown, analytical techniques utilising genetic data can provide information to management. The application of these techniques has typically been limited to species with restricted dispersal or to localised geographic scales. Here these techniques are used in conjunction with traditional approaches to study the invasion of a highly vagile species over a continental scale. Originally introduced to eastern Australia, the common starling (Sturnus vulgaris) is expanding westward and has reached the southeast agricultural region of Western Australia (WA). Spatial, temporal and museum samples representing historical incursions into WA were used in genetic analyses to answer questions important to WA management agencies. Where possible, multiple marker classes were used to evaluate their relative ability to address these questions, including microsatellites, mitochondrial DNA (mtDNA), and a nuclear gene. Between two and four genetic groups were identified in Australia, and the localities on the edge of the range expansion (ERE) were consistently genetically differentiated from all others. Levels of genetic exchange between groups appeared to be low, suggesting that localised control may be effective. Analyses of historical samples indicated patterns of genetic exchange are temporally stable. This suggests that starlings will continue to arrive from the east, requiring vigilance from management. Consistently, a negative relationship was found between genetic diversity and date of first record, which may help in the future to distinguish ineffective surveillance from recent colonisations. Genetic evidence of female-biased dispersal indicated that control strategies preferentially targeting females may more efficiently limit dispersal than those targeting both sexes equally. Variants private to particular genetic groups were identified; tracking the spread of these variants may assist future monitoring programs in understanding ongoing exchange between groups. One mtDNA variant private to ERE localities showed dramatic temporal change, which could not be adequately explained by genetic drift or admixture, but may be explained by selection. Microsatellites were most useful in determining origins and identifying dispersers, mtDNA sequences provided a unique tool for ongoing monitoring of dispersal, and nuclear sequences had high levels of gene diversity and resolution of population structure.

Invasive range expansion is correlated to life- history variation, boldness and dispersal behavior. The invasion of the African Jewelfish in Everglades National Park provides an opportunity to test life-history trait variation, boldness and dispersal behavior in the invasion success of this species. My study examined variation in somatic traits, boldness, and dispersal of jewelfish across their invaded range. Life histories were examined on wild individuals. Boldness and dispersal were tested in outdoor experimental tanks. Tested populations from the invasion front have higher somatic traits, but they were not bolder than longer established populations. Understanding the underlying mechanisms of invasions are key for the development of strategies looking to contain invaders and prevent their spread.

No description available.

No description available.

Coastal forests are facing threats due to rising sea levels, increased storm severity, and land use change. These factors stress trees within coastal ecosystems, potentially predisposing them to attack by insects. In North America, two insect species of concern that pose threats to for coastal forest health are the native baldcypress leafroller (BCLR), Archips goyerana Kruse (Lepidoptera: Tortricidae), and non-native Japanese cedar longhorned beetle (JCLB), Callidiellum rufipenne Motschulsky (Coleoptera: Cerambycidae). Baldcypress leafroller has been reported from Mississippi and Louisiana, with defoliation only reported from the latter where increased flooding has stressed host trees. Through widespread trapping efforts, I found that BCLR occurs north into Arkansas and northeast into Delaware. I conducted environmental niche modeling that indicated that climatically suitable habitat in the United States is primarily in the southeastern United States, but that climatic suitability of the southeastern United States will increase and expands northwards. For JCLB, I found that interceptions of this insect of ports of entry in North America have remained low since the implementation of ISPM-15, and that most interceptions occur on wood packaging materials from the insect’s native range. Environmental niche modeling for JCLB indicated that climatically suitable areas were more prevalent in in the northern hemisphere under current and future climatic scenarios, but that poleward shifts in suitability are likely with ongoing climate change.

Doctor of Philosophy / Department of Biology / Samantha Wisely / Habitat fragmentation and destruction are the most ubiquitous and serious environmental threats confronting the long-term survival of plant and animal species worldwide. However, some native or exotic species can take advantages of these alterations and expand their range, placing endemic species at risk of extinction by changing the composition of biotic communities and altering ecosystem. Capybara (Hydrochoerus hydrochaeris) are a widely distributed rodent throughout most of South and Central America, but restricted to areas of standing water. As the Gran Chaco ecosystem of Paraguay has been converted from dry tropical forest to pastureland, I hypothesized that this habitat alteration created potential for invasion by capybara into newly fragmented areas. I used ecological niche modeling to generate hypotheses about how the distribution of capybara has been affected by land use change, and tested those hypotheses with phylogeographic analyses. To understand the mechanisms that have allowed the invasion, I investigated home range, habitat use and thermoregulation of capybara via radiotelemetry in a deforested area in which capybara had recently invaded. Genetic analyses confirm a rapid range expansion scenario with evidence of secondary contact between two distinct phylogroups which had previously been disjunct. Modeling results indicated that conversion of forest to pastureland allowed the expansion to occur. Capybara selected water significantly more than it was available to them, and avoided shrub forest. I found a significant positive correlation between body temperature and distance from water, and a significant negative correlation between distance from water and Chaco ambient temperature. Capybara proximity to water appeared to be tightly linked to body thermoregulation. These results suggest that although capybara have expanded into the Chaco forest as it is converted to pastureland, the presence of permanent water sources in those pastures are the mechanism that allow capybara to persist in this habitat. This is the first study to characterize capybara in a xeric habitat without a year round water source, and scarce natural grasslands. My results show how anthropogenic habitat modification has allowed capybara to thrive. Understanding how capybara invade and utilize the deforested Central Dry Chaco will provide valuable information for the future management of the species and the Chaco ecosystem.

Submitted by Franciele Moreira (francielemoreyra@gmail.com) on 2018-03-23T12:55:32Z No. of bitstreams: 2 Dissertação - Herlander Correia de Lima - 2018.pdf: 2848519 bytes, checksum: 21c8989e0952abc6dc4f229fa27ff46f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-03-23T14:58:47Z (GMT) No. of bitstreams: 2 Dissertação - Herlander Correia de Lima - 2018.pdf: 2848519 bytes, checksum: 21c8989e0952abc6dc4f229fa27ff46f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-03-23T14:58:47Z (GMT). No. of bitstreams: 2 Dissertação - Herlander Correia de Lima - 2018.pdf: 2848519 bytes, checksum: 21c8989e0952abc6dc4f229fa27ff46f (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-21 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Research in animal personality is increasing our understanding of what prevents a species from colonizing new areas, which is one of the outstanding questions in biogeography. Some behavioral types can perform better than others in specific stages involved in range expansion. For example, a high exploratory behavior increases the chances of finding new resources in novel environments. However, inconsistent results in the literature hindered a definite recognition of the role of animal personalities on species distributions. I collected data available in the literature and performed a bayesian meta-analysis to assess which behavioral types are driving range expansion in the following biogeographical processes: dispersal, migration and invasion. I used several moderators to try to discern context-dependencies in effect sizes. A hierarchical model, with effect sizes nested within studies, revealed that more exploratory and bolder behaviors facilitate range expansion. Also, I found that invasive individuals are more likely to be more exploratory and more active than natives, while dispersers are generally bolder and more exploratory than non-dispersers. Yet, the low study sample size obtained for analysis stresses the need to conduct more primary studies. Results highlight the role of behavioral traits in species distributions and increase our knowledge about which ecological characteristics might prepare species to endure the current global environmental challenges. / A pesquisa em personalidade animal está aumentando o nosso conhecimento sobre o que previne uma espécie de colonizar novas áreas, sendo esta uma das principais questões em biogeografia. Alguns tipos de comportamento podem resultar em melhor desempenho que outros em específicos estágios de expansão do território. Por exemplo, um comportamento mais exploratório facilita a descoberta de recursos em um novo meio. Contudo, resultados inconsistentes na literatura estão dificultando um reconhecimento do papel da personalidade animal na distribuição das espécies. Coletei dados da literatura e realizei uma meta-análise bayesiana para determinar que tipos de comportamento são responsáveis pela expansão do território através dos processos biogeográficos de: dispersão, invasão e migração. Fiz ainda uso de vários moderadores na tentativa de identificar contexto-dependências nos tamanhos de efeito. Em um modelo hierárquico, usando tamanhos de efeito aninhados dentro dos estudos, mostro que um comportamento mais ousado e mais exploratório facilita o sucesso na expansão do território. Para além disso, eu demonstro que invasores são mais exploratórios e mais ativos que nativos, e dispersores são mais exploratórios e ousados que não-dispersores. Contudo, o baixo tamanho amostral obtido para as análises demonstra a necessidade de conduzir mais estudos primários. Os resultados realçam o papel dos traços comportamentais na distribuição das espécies e aumentam o nosso conhecimento sobre que características ecológicas podem preparar as espécies para resistir aos desafios das mudanças ambientais.