Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-02-17T19:43:31Z No. of bitstreams: 1 DanielChavesDeLima_TESE.pdf: 22514409 bytes, checksum: 31f3c8faf388b52b979837aa634b9a04 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-02-20T18:50:56Z (GMT) No. of bitstreams: 1 DanielChavesDeLima_TESE.pdf: 22514409 bytes, checksum: 31f3c8faf388b52b979837aa634b9a04 (MD5) / Made available in DSpace on 2017-02-20T18:50:56Z (GMT). No. of bitstreams: 1 DanielChavesDeLima_TESE.pdf: 22514409 bytes, checksum: 31f3c8faf388b52b979837aa634b9a04 (MD5) Previous issue date: 2016-09-23 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Chromobacterium violaceum is a ?-proteobacteria commonly found around tropical and subtropical regions throughout the globe. It produces many metabolites with biotechnological properties such as antitumoral peptides, antibiotics and polymers that have potential to replace the oil-based ones. Although it has been extensively studied over the past 40 years, there are many aspects of C. violaceum that remains unclear until today. We have conducted a biochemical study on the homologous recombination (HR) machinery of C. violaceum, mainly in RecA and its paralog, RadA/Sms. We performed in vitro assays from initial and late steps of HR such as D-loop formation and branch migration, respectively, with their corresponding molecular actors and how RadA/Sms influenced each one. We observed cvRadA/Sms influences negatively D-loop formation promoted by cvRecA and through pull-down assay we have observed an interaction between these two proteins. We also observed the DNA-binding preference of cvRadA/Sms and cvRecA and observed that this protein binds preferentially to dsDNA instead ssDNA, unlike cvRecA. No involvement of cvRadA/Sms on branch migration reactions was detected. In this work, we also described, for the first time, the isolation, sequencing and annotation of a new plasmid from C. violaceum, which we named ChVi1 and has 44,236 base pairs, 39 predicted open reading frames (ORFs) and, possibly, two origins of replication. Most of the ORFs codes for hypothetical and structural bacteriophage proteins. By using restriction digestion and Next-generation sequencing (NGS) we also looked for the presence of a similar plasmid in other seven C. violaceum strains isolated from amazon region. Our analysis suggest the presence of a plasmid similar to ChVi1 in two of these strains. The present work describes for the first time a biochemical characterization of RadA/Sms and RecA from C. violaceum which have different roles in HR. Moreover, the discovery of ChVi1 opens a path to further explore C. violaceum?s biology.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-03T22:56:48Z No. of bitstreams: 1 LeonardoWaylandTorresSilva_TESE.pdf: 3255016 bytes, checksum: 1c9f68f3968c7c1bdbdc4119bde6f919 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-05-05T20:16:14Z (GMT) No. of bitstreams: 1 LeonardoWaylandTorresSilva_TESE.pdf: 3255016 bytes, checksum: 1c9f68f3968c7c1bdbdc4119bde6f919 (MD5) / Made available in DSpace on 2016-05-05T20:16:14Z (GMT). No. of bitstreams: 1 LeonardoWaylandTorresSilva_TESE.pdf: 3255016 bytes, checksum: 1c9f68f3968c7c1bdbdc4119bde6f919 (MD5) Previous issue date: 2015-06-19 / Os centros de lan?amento e rastreio t?m por finalidade realizar atividades cient?ficas e comerciais com ve?culos aeroespaciais. Os Sistemas de Rastreio de Foguetes (SRF) integram a infraestrutura desses centros, sendo respons?veis pela coleta e processamento dos dados da trajet?ria dos ve?culos. Os sensores dos SRFs normalmente s?o Radares com Refletores Parab?licos (RRPs), mas tamb?m ? poss?vel usar radares com arranjos de antenas, chamados de Arranjos de Varredura (AVs), originando os Radares com Arranjos de Varredura (RAVs). Nos AVs, o sinal de alimenta??o de cada elemento radiante do arranjo pode ser ajustado para fazer o controle eletr?nico do diagrama de radia??o, a fim de aumentar as funcionalidades e reduzir as manuten??es do sistema. Com isso, nos projetos de implanta??o e reutiliza??o de RAVs, a modelagem est? sujeita a v?rias combina??es de sinais de alimenta??o, produzindo um problema de otimiza??o complexo, devido ao grande n?mero de solu??es dispon?veis. Para solucionar tal problema, ? poss?vel usar m?todos de otimiza??o off-line, tais como Algoritmos Gen?ticos (AGs), cujas solu??es calculadas s?o armazenadas para aplica??es on-line. Nesse contexto, o m?todo do Algoritmo Gen?tico com Crossover M?ximo-M?nimo (Genetic Algorithm with Maximum-Minimum Crossover - GAMMC) foi usado para desenvolver o algoritmo GAMMC-P, que otimiza a etapa de modelagem do controle do diagrama de radia??o de AVs planares. Comparado a um AG com recombina??o convencional, o GAMMC tem uma abordagem diferente, pois realiza a recombina??o de indiv?duos mais aptos com indiv?duos menos aptos, para aumentar a diversidade gen?tica da popula??o, evitando a converg?ncia prematura, aumentando o fitness e reduzindo o tempo de processamento. Assim, o GAMMC-P utiliza um algoritmo reconfigur?vel, com m?ltiplos objetivos, codifica??o real diferenciada e o operador gen?tico MMC, tendo atingido com sucesso os requisitos propostos para diferentes condi??es de opera??o de um RAV planar. / Launching centers are designed for scientific and commercial activities with aerospace vehicles. Rockets Tracking Systems (RTS) are part of the infrastructure of these centers and they are responsible for collecting and processing the data trajectory of vehicles. Generally, Parabolic Reflector Radars (PRRs) are used in RTS. However, it is possible to use radars with antenna arrays, or Phased Arrays (PAs), so called Phased Arrays Radars (PARs). Thus, the excitation signal of each radiating element of the array can be adjusted to perform electronic control of the radiation pattern in order to improve functionality and maintenance of the system. Therefore, in the implementation and reuse projects of PARs, modeling is subject to various combinations of excitation signals, producing a complex optimization problem due to the large number of available solutions. In this case, it is possible to use offline optimization methods, such as Genetic Algorithms (GAs), to calculate the problem solutions, which are stored for online applications. Hence, the Genetic Algorithm with Maximum-Minimum Crossover (GAMMC) optimization method was used to develop the GAMMC-P algorithm that optimizes the modeling step of radiation pattern control from planar PAs. Compared with a conventional crossover GA, the GAMMC has a different approach from the conventional one, because it performs the crossover of the fittest individuals with the least fit individuals in order to enhance the genetic diversity. Thus, the GAMMC prevents premature convergence, increases population fitness and reduces the processing time. Therefore, the GAMMC-P uses a reconfigurable algorithm with multiple objectives, different coding and genetic operator MMC. The test results show that GAMMC-P reached the proposed requirements for different operating conditions of a planar RAV.

Made available in DSpace on 2014-12-17T15:15:05Z (GMT). No. of bitstreams: 1 HidalynTCMS.pdf: 3137452 bytes, checksum: c9101961e25b2af76cdd7d74bfe14bb5 (MD5) Previous issue date: 2007-04-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Understanding the way in which large-scale structures, like galaxies, form remains one of the most challenging problems in cosmology today. The standard theory for the origin of these structures is that they grew by gravitational instability from small, perhaps quantum generated, ?uctuations in the density of dark matter, baryons and photons over an uniform primordial Universe. After the recombination, the baryons began to fall into the pre-existing gravitational potential wells of the dark matter. In this dissertation a study is initially made of the primordial recombination era, the epoch of the formation of the neutral hydrogen atoms. Besides, we analyzed the evolution of the density contrast (of baryonic and dark matter), in clouds of dark matter with masses among 104M? ? 1010M?. In particular, we take into account the several physical mechanisms that act in the baryonic component, during and after the recombination era. The analysis of the formation of these primordial objects was made in the context of three models of dark energy as background: Quintessence, ?CDM(Cosmological Constant plus Cold Dark Matter) and Phantom. We show that the dark matter is the fundamental agent for the formation of the structures observed today. The dark energy has great importance at that epoch of its formation / Entender o modo pelo qual estruturas em grande-escala, como gal?xias, se formam permanece um dos problemas mais desafiadores em cosmologia hoje. A teoria padr?o para a origem destas estruturas ? que elas cresceram por instabilidade gravitacional de pequenas, talvez geradas quanticamente, flutua??es na densidade de mat?ria escura, b?rions e f?tons sobre um Universo primordial uniforme. Depois da recombina??o, os b?rions come?aram a cair nos po?os de potencial gravitacional pr?-existentes de mat?ria escura. Nesta disserta??o ? feito inicialmente um estudo da era da recombina??o primordial, a ?poca da forma??o dos ?tomos de hidrog?nio neutro. Al?m disso, analisamos a evolu??o do contraste de densidade (de mat?ria bari?nica e escura), em nuvens de mat?ria escura com massas entre milhares e bilh?es de massas solares. Em particular, na an?lise da componente bari?nica, levamos em conta os v?rios mecanismos f?sicos que ocorrem nela durante e depois da era da recombina??o. A an?lise da forma??o desses objetos primordiais foi feita no contexto de tr?s modelos de energia escura como background ': Quintess?ncia, CDM (Constante Cosmol?gica mais Mat?ria Escura Fria) e Fantasma. Mostramos que a mat?ria escura ? o agente fundamental para forma??o das estruturas observadas hoje. A energia escura tem grande import?ncia na ?poca de sua forma??o