Final thesis was worked out on theme Aplication of cryotherapy as a tool for plant viruses eradication from Prunus persica (L.) Batsch regenerants. Experiment was conducted on Faculty of Agronomy of the Mendel University in Brno and in the Crop Research Institute in Prague-Ruzyně. Literary research describes information about peach cultivation in vitro and plant pathogen eradication by cryotherapy. These information has been used in practical part of the final thesis. Plant material of the ´Redhaven´variety of peach was used for virus eradication by cryotherapy. Cryotherapy was applied on shoot tips. In the end of final thesis shoot tips viability and regeneration were evaluated.