The diploma thesis deals with the analysis of problems of unevenly stratified air flow in air handling units and subsequent optimization with respect to speed ratios, energy efficiency and legislative requirements. Methods of solving the problem are theoretical and experimental, while the theoretical part deals with air distribution by means of air-conditioning fans with a focus on their aerodynamics, pressure conditions and other requirements, which are an integral part of properly designed equipment. In the experimental part of the thesis was applied a proposal of an innovative technical approach to a specific model of air conditioning assembly, which was implemented in the laboratories of the Institute of Building Services.