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Diagnose nutricional de enxofre em eucalipto por an?lise n?o destrutiva / Nutritional diagnosis in eucalyptus by non-destructive analysisChaves, Carolina Mata Machado Barbosa 09 June 2017 (has links)
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Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / O enxofre (S) ? um nutriente essencial para crescimento e metabolismo das plantas, e sua defici?ncia pode limitar a produtividade de muitas culturas. A adapta??o de t?cnicas espec?ficas de an?lise espectral para a determina??o do estresse nutricional causado por este nutriente pode permitir a detec??o precoce da sua defici?ncia e suplementa??o de precis?o na aduba??o corretiva das plantas. Esse experimento foi conduzido com o objetivo de determinar se a reflect?ncia foliar obtida atrav?s do mini espectr?metro pode ser utilizada para detectar a defici?ncia de S em mudas de Eucalyptus urophylla. Durante 90 dias as mudas foram cultivadas em solu??o nutritiva de Clark modificada completa (sem omiss?o de S) e com omiss?o de S. Os sintomas visuais de defici?ncia de S foram observados e fotografados semanalmente. A cada 15 dias foram avaliados altura e di?metro das plantas e leituras nas folhas diagn?sticos, com o mini espectr?metro. Ao final de cada avalia??o, amostras de folhas diagn?sticos foram preparadas e submetidas ?s an?lises qu?micas do material vegetal dos teores de nutrientes, posteriormente as mudas foram separadas em ra?zes, caule e folhas para obten??o da massa seca total e dos seus componentes. Ap?s calcular a primeira derivada das reflect?ncias obtidas nas leituras com o mini espectr?metro, obteve-se a posi??o dos pontos de inflex?o. Foi realizado ainda um estudo de regress?o linear m?ltipla entre os teores de nutrientes das folhas diagn?sticos como vari?veis dependentes, e a produ??o de massa seca da parte a?rea e valores obtidos para o IPP como vari?veis independentes. A defici?ncia de S causou altera??es nas propriedades morfol?gicas e fisiol?gicas das plantas de Eucalyptus, o que refletiu nas mudan?as das propriedades espectrais foliares da esp?cie em quest?o. Os sintomas visuais de defici?ncia de S iniciaram-se aos 28 dias ap?s a aplica??o dos tratamentos (DAT), intensificando-se ao longo do experimento. Foi observado clorose nas folhas novas, caule mais delgado e redu??o no crescimento das mudas quando comparados ao tratamento completo. A an?lise nutricional detectou redu??o nas concentra??es de S foliar e defici?ncia do nutriente nas plantas aos 45 DAT. Contudo, somente aos 75 dias a defici?ncia de S alterou a reflect?ncia das mudas com omiss?o de S, deslocando o ponto de inflex?o para comprimentos de ondas mais curtos (? 700 ?m), ou seja, quando o aparelho (mini espectr?metro) conseguiu detectar defici?ncia nutricional de S nas plantas de Eucalyptus. A defici?ncia de S tamb?m reduziu a altura, di?metro e produ??o de massa seca total e dos seus componentes aos 75 DAT. A posi??o do ponto de inflex?o e a massa seca da parte a?rea apresentaram alta correla??o com a concentra??o de S foliar. As an?lises de regress?o linear m?ltipla e de correla??o simples indicaram que os valores de reflect?ncia foliar estimados pelo mini espectr?metro e pela massa seca da parte a?rea estavam estreitamente correlacionados com as concentra??es de S nas folhas das mudas de Eucalyptus (r2=0,99). Os resultados obtidos sugerem que a reflect?ncia espectral demonstra alto potencial como ferramenta diagn?stico para estimar a concentra??o de S foliar em Eucalyptus por meio do mini espectr?metro. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / Sulfur (S) is an essential nutrient for plant growth and metabolism, its deficiency can limit the productivity of many crops. The adaptation of specific remote sensing and spectral analysis techniques for the determination of nutrient stress in plants could allow early detection and precise supplementation for corrective fertilization. This experiment was conducted with the objective of determining if the leaf reflectance obtained through the mini spectrometer can be used to detect S deficiency in Eucalyptus urophylla seedlings. During 90 days the seedlings were grown in complete nutrient solution of Clark (without omission of S) and with omission of S. The visual symptoms of S deficiency were observed and photographed weekly. Every 15 days, plants height and diameter were evaluated, and readings on the diagnostic leaf were taken with the mini spectrometer. At the end of each evaluation the samples of the diagnostic leaves were prepared and submitted to the chemical analyzes of nutrient contents of the plant material, later the seedlings were separated into roots, stem and leaves to obtain the mass of total dry matter and its components. After calculating the first derivative of the reflectances obtained in the readings with the mini-spectrometer, the position of the inflection points was obtained. A multiple linear regression study was carried out between the nutrient contents of the diagnostic leaves as dependent variables and the shoot dry matter yield and values obtained for the position of the inflection points as independent variables. Sulfur deficiency caused changes in the morphological and physiological properties of Eucalyptus plants, which reflected changes in the leaf spectral properties of the species. The visual symptoms of S deficiency began at 28 days after application of the treatments, intensifying throughout the experiment. It was observed chlorosis in the new leaves, slender stem and reduction in the growth of the seedlings when compared to the complete treatment. Nutritional analysis detected a reduction in the foliar S concentrations and nutrient deficiency in plants at 45 days after application of the treatments. However, only at 75 days S deficiency altered the reflectance of the seedlings under S omission, shifting the inflection point to shorter wavelengths (? 700 ?m),that is, when the device (mini spectrometer) was able to detect nutritional deficiency of S in Eucalyptus plants. Sulfur deficiency also reduced the height, diameter and yield of total dry mass and its components at 75 days after application of the treatments. The position of the inflection point and the shoot dry mass presented a high correlation with the concentration of leaf sulfur. Multiple linear regression and simple correlation analyzes indicated that leaf reflectance values estimated by the mini spectrometer and through MSPA were closely correlated with the concentrations of S in the leaves of the Eucalyptus seedlings (r2 = 0.99).The results obtained suggest that the spectral reflectance demonstrates high potential as a diagnostic tool to estimate the leaf sulfur concentration in Eucalyptus using the mini-spectrometer.
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Estimativa de nutrientes foliares em mudas de eucalipto pelo uso de mini espectr?metro / Foliar nutrients estimates in eucalyptus seedling using mini spectrometerSarmento, Mateus Felipe Quintino 10 March 2017 (has links)
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Previous issue date: 2017 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / A disserta??o foi dividida em dois cap?tulos, o primeiro referente ao embasamento te?rico da resposta vegetal a radia??o eletromagn?tica na regi?o do vis?vel e infravermelho pr?ximo e a rela??o da reflect?ncia com a estimativa de caracter?sticas de maneira n?o destrutiva por meio do uso de ?ndices de vegeta??o. O segundo sobre o uso de novos ?ndices de vegeta??o NI, PI, KI, CaI, MgI, SI, MnI, FeI, ZnI, CuI e BI na estimava do teor de macro e micronutrientes em eucalipto nas condi??es de viveiro, por meio do uso da reflect?ncia espectral. No trabalho foram coletados dados de reflect?ncia espectral, em folhas isoladas, nos materiais gen?ticos AEC 2034 ((Eucalyptus camaldulensis Dehnh x Eucalyptus grandis Hill (ex Maiden)) x Eucalyptus urophylla S.T. Blake) e AEC 2475 (Eucalyptus urophylla S.T. Blake x Eucalyptus pellita F. Muell) em minijardim clonal e produ??o de mudas aos 65 dias de idade. Os dados espectrais foram coletados com o mini espectr?metro foliar port?til CI-710 (CID, Inc., Camas, Washington, USA). As curvas espectrais no minijardim clonal foram estatisticamente iguais na faixa de 400 a 900, e estatisticamente diferentes na regi?o do violeta e azul e infravermelho pr?ximo. Na produ??o de mudas ?s curvas espectrais foram estatisticamente diferentes na faixa de 400 a 900 nm e em todas as regi?es avaliadas. Os ?ndices de vegeta??o apresentaram boas estimativas nas condi??es de minijardim clonal e produ??o de mudas, sendo poss?veis as estimativas por meio de ?ndices de vegeta??o no minijardim clonal e produ??o de mudas. Os ?ndices de vegeta??o que apresentaram as melhores estimativas do teor de nutrientes nas condi??es de minijardim clonal e produ??o de mudas foram os que atuam na regi?o do red edge. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Ci?ncia Florestal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / This work has been divided into two chapters, the first one refers to theoretical support regarding vegetation?s reaction to eletromagnetic radiation in the area of visibility and infrared surrounding it, and the relation of reflectance with an estimate of non-destructive characteristics by using vegetation indices. The second chapter is about the usage of new vegetal indices NI, PI, KI, CaI, MgI, SI, MnI, FeI, ZnI, CuI and BI in the estimate of macro and micronutrients level in eucalyptus under nursery tree conditions by using spectral reflectance. During the research, data from spectral reflectance was collected from isolated leaves, in the genetical material AEC 2034 ((Eucalyptus camaldulensis Dehnh x Eucalyptus grandis Hill (ex Maiden)) x Eucalyptus urophylla S.T. Blake) and AEC 2475 (Eucalyptus urophylla S.T. Blake x Eucalyptus pellita F. Muell) in clonal mini-garden and seedlings production of 65 days old. Spectral data was collected using a portable foliar spectrometer CI-710 (CID, Inc., Camas, Washington, USA). The spectral curves in the clonal mini-garden were statistically equal from 400 to 900 length, and statistically different in the violet, blue and ifrared surrounding area. In the seedlings production the spectral curves were statistically different from 400 to 900 nm and in all evaluated area. The vegatation indices presented good estimates under the conditions of clonal mini-garden and seedlings production, considering that the estimates were possible due to the vegetation indices in the clonal mini-garden and seedling production. The vegetation indices that showed the best estimate of nutrients content under clonal mini-garden conditions and seedlings production were the ones situated in the red edge area.
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