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The damage potential of the root-lesion nematode Pratylenchus bolivianus in the UKBarker, Anthony David Purslove January 1999 (has links)
No description available.
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Some sampling issues in econometricsRamalho, Esmeralda A. January 2002 (has links)
No description available.
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Mg and Ca isotope fractionation during CaCO₃ biomineralisationChang, Veronica Tzu-Chun January 2002 (has links)
No description available.
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The analysis of sediment reference materials by direct sample insertion inductively coupled plasma atomic emission spectrometryBlain, Laurent January 1990 (has links)
Note:
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A tumbler and pore water expression device to prepare homogeneous samples for the extraction of free chloride in cement pasteDelport, D.J., Potgieter-Vermaak, S.S., Potgieter, J.H. January 2013 (has links)
Published Article / Corrosion of rebar in concrete is commonly associated with the free chloride in the pore water in the cement matrix. Knowing the quantity of chloride in concrete is important because chloride can promote corrosion of steel reinforcement when moisture and oxygen are present. The problem ofphysical extraction and the measurement of the free chloride content in pore water solutions extracted from cement pastes has received attention in literature but has not been explained in full detail. However, the variability of results obtained from the different methods used by various investigators only serves to confuse the issue. This investigation describes the use of a tumbler designed to prepare homogeneous samples and the use of a pore water expression device designed to extract free chloride in cement paste and concrete samples.
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Dental microwear and diet in Griphopithecus alpaniKing, Tania Christine January 1997 (has links)
No description available.
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An investigation of the performance characteristics of isothermal calorimetersJones, Andrew Christopher January 1997 (has links)
No description available.
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DETERMINATION AND SPECIATION OF TELLURIUM IN ENVIRONMENTAL SAMPLES USING HYDRIDE GENERATION ATOMIC FLUORESCENCE SPECTROSCOPY (HG-AFS)Alzahrani, Ali 27 January 2014 (has links)
This thesis focuses on developing a new method to measure trace tellurium (Te) in
different environmental samples such as lake waters, mine tailings and sediments. The developed
technique is based on Hydride Generation Atomic Fluorescence Spectroscopy (HG-AFS), a
technique that can measure low concentration of Te and also allows for Te speciation at low cost
and high efficiency in various environmental samples.
To validate the method that could be used to determine Te speciation in various types of
environmental samples, a series of tests has been designed for finding the best conditions to
measure Te(IV) using HG-AFS and obtain accurate and reliable results. Those tests include the
stability of the signal, the acidity of the solution, the volatility of Te after digestion of solids, the
reduction from Te(VI) to Te(IV), the detection limit of the technique, and the validity of two
digestion methods under the optimum (HG-AFS) instrumental settings.
An interference study including the most common elements in the Earth’s crust such as
(Ni, Fe, Pb, Cr, Cu, Co, Zn, Mn and Mo) was also performed. The results of this study showed
that Cu(II) can severely interfere with Te quantification decreasing the Te signal to almost zero.
Therefore, different masking agents such as 8-hydroxyquinoline, 1,10-phenanthroline, urea and
thiourea were tested to reduce and eliminate this interference.
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Human health risk assessment for contaminated land in historical mining areasTristan-Montero, Emma Esther January 2000 (has links)
No description available.
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Using oligonucleotide signatures to build a system for effective detection of pathogenic bacteria in metagenomic samplesEmmett, Warren Anthony 11 August 2009 (has links)
Pathogenic bacteria are responsible for millions of deaths every year with an estimated mortality of 70 million people by 2010 for Mycobacterium tuberculosis alone. Novel methods for identification of bacterial species in hosts, urban environments, water sources and food stuffs are required to advance diagnosis and preventative medicine. Detection of bacterial species in environmental samples is a complex task since large numbers of bacteria are present and are resistant to culturing. Therefore, the genetic content of the entire sample has to be analysed simultaneously and this constitutes a metagenomic sample. Commonly-used methods of bacterial identification focus on detection of specific genomic regions to determine species. Currently only one percent of a metagenomic sample can be used for identification employing phylogenetic markers. This method is highly inefficient. The search for more widespread markers within each genome is essential to improve detection methods. Also, modern sequencing technologies used in these environments have short read lengths which prove difficult to assemble e.g. repeats can lead to incorrect assembly. The use of overrepresented oligonucleotides provides a solution to both of these difficulties. Overrepresented oligonucleotides (8-14bp in length) are utilised to differentiate between species based on observed frequency of occurrence rather than presence or absence. They occur throughout the genome thereby increasing genomic coverage. Furthermore, overrepresented oligonucleotides can be easily identified in a raw metagenomic sample, bypassing the need for sequence assembly. Raw oligonucleotide data was filtered, analysed and imported into a structured database. A program, Oligosignatures, allowed for creation of species and phylogenetic lineage specific oligonucleotide markers dependent on the selection of species specified by the user. For the purposes of this study, the context of bacterial identification in an unknown environment was selected. A similarity trial was then executed to determine if strains of the same species can be separated from each other using overrepresented oligonucleotides. Outcomes of this test provided a guideline for the creation of species and lineage specific oligonucleotide markers. Each species and lineage was therefore described by a marker profile which consisted of representative oligonucleotide markers. These marker profiles were then tested against artificial and experimental data to determine their effectivity. Two approaches were used for testing, namely Oligonucleotide frequency analysis and Sequence read analysis. Oligonucleotide frequency analysis focused on the identification of species dependent on the global frequencies of marker oligonucleotides within each marker profile. Sequence read analysis attempted to assign metagenomic reads to a specific species dependent on the number of marker oligonucleotides present within the read. The final database contained 439 bacterial genomes from 22 different phylogenetic lineages. Interpretation of the results obtained after strain similarity testing showed that strains of the same species had highly similar markers and were not separable using this approach. All strains of a species that conformed to this premise were reduced to a single representative member. Similarly, species marker profiles demonstrated that closely related species remained difficult to separate. Twenty-one of the 22 lineages showed sufficient lineage specific markers for use in testing. This provides support for the abundance of overrepresented oligonucleotides and their potential for use as a detection method. In general, metagenomic testing of marker profiles showed that species specific determination was prone to interference, specifically, in closely related species. However, more distantly related species could be separated using both methods. Lineage discrimination generated more reliable results proving that lineage determination was possible in both artificial and experimental datasets. Oligonucleotide frequency analysis, the most sensitive approach, showed the best results for lineage determination but poorer results for species identification. Sequence read analysis provided a more effective method of determining confidence using different thresholds for read classification. In conclusion, the use of overrepresented oligonucleotides holds promise as a novel method for bacterial identification in a metagenomic context. Although several obstacles still prevent optimal utilization of these oligonucleotides, with further research the classification and identification of species and phylogenetic lineages from metagenomic samples can become a reality. Copyright / Dissertation (MSc)--University of Pretoria, 2009. / Biochemistry / unrestricted
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