Toxic/mutagenic potentials of Gambierdiscus toxicus (GT) and Prorocentrum lima (PL) methanol extracts (CME) were determined using brine shrimp (Artemia salina), mouse, chicken embryo and Salmonella microsomal assays. PL-CME and GT-CME were toxic to shrimp and mice. Isolation and separation were accomplished using ether/water, hexane/methanol partition and butanol extraction. Toxic fractions were purified using column and thin layer chromatography (TLC). GT-CME showed low levels of mutagenic potential. For GT isolated fractions and PL-CME, no mutagenic effects were observed. Both CMEs showed embryotoxicity, with no teratogenic effects. Ether/methanol and water/butanol fractions showed shrimp toxicity. These fractions were purified by treatment with warm/cold acetone. Acetone insoluble precipitates were obtained. Ether soluble acetone filtrate (ESAF) and butanol soluble acetone precipitate (BSAP) showed shrimp and mouse toxicity. GT-BSAP produced temperature depression in mice. Three toxic isolates were obtained from PL-ESAF, four from GT-ESAF and one from both BSAPs columns. TLC preparative plates showed at least 12 toxic isolates for PL-ESAF, 8 for GT-ESAF and 4 for GT-BSAP.

Thesis (M. Sc.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf i-ix).

Thesis (M. Sc.)--University of Hong Kong, 1996. / Includes bibliographical references (leaf 57-60).

Thesis (Ph.D.)--The Ohio State University, 2007. / Adviser: Timothy Haab. Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 118-123).

published_or_final_version / Environmental Management / Master / Master of Science in Environmental Management

Thesis (M.S.)--Oregon State University, 2008. / Printout. Includes bibliographical references (leaves 78-86). Also available on the World Wide Web.

published_or_final_version / Environmental Management / Master / Master of Science in Environmental Management