The purpose of this study was to evaluate the effect of a final rinse of ethanol on depth of sealer penetration in teeth obturated with Gutta Percha (GP)/AH-Plus® (Dentsply, De Trey GmbH, Konstanz, Germany) or Resilon/Epiphany® SE™ (Pentron Clinical Technologies, LLC, Wallingford, CT). Extracted human anterior teeth (n= 32) were shaped to size 30, 0.06 taper using nickel-titanium rotary files and subjected to an identical irrigation protocol. Specimens were randomly divided into eight groups according to final irrigating solution (saline, 70%, 95%, or 100% ethanol) and obturation material (GP/AH-Plus® or Resilon/EpiphanyÒ SE™). A 2mm thick slice was obtained by sectioning each obturated root at 3mm and 5mm from the anatomic apex. Specimens were cleared and assessed using scanning electron microscopy (SEM). Sealer penetration was observed at different magnifications when using GP/AH Plus® across all final rinse concentrations. Among Resilon/Epiphany® SE™ groups, no sealer penetration was evident under SEM. Conclusions: 1) GP/AH-Plus® showed evidence of sealer penetration, however, Resilon/Epiphany® SE™ did not show evidence of sealer penetration at both the dentin and sealer interface. 2) A final rinse with any concentration of ethanol prior to obturation does not improve sealer penetration with GP/AH-Plus® groups. 3). Resilon/Epiphany® SE™ bond can be dislodged at either the interface of sealer and Resilon or dentin and Resilon.