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Neste trabalho estudou-se um novo arranjo de medida para se obter a distribuição de energia dos elétrons secundários emitidos e os coeficientes de emissão por um sólido. Pelo fato deste arranjo permitir a medida direta do potencial de superfície no caso dos polímeros e da ddp de contato no caso dos metais, foi possível aplicar e descrever teoricamente o método de medida empregado para determinar o espectro energético dos secundários para a platina, Teflon-FEP, Mylar, PVDF- α, PVDF- β e polimero condutor. As cargas acumuladas durante a irradiação dos polímeros foram compensadas através do próprio feixe eletrônico. Este procedimento não é muito usual em relação aqueles aplicados na literatura e mostrou-se bastante eficaz. Pela primeira vez empregou-se o método dinâmico para determinar o coeficiente de emissão total em função da energia dos elétrons incidentes nos metais e verificou-se que estes resultados são idênticos aqueles obtidos pelo método do pulso. Um desdobramento natural ao aplicar o método dinâmico aos metais foram as medidas realizadas para camadas de ouro com espessura de 50 nm, 10 nm e 2,5 nm depositada sobre o Teflon-FEP, que deram origem ao modelo das duas camadas na interpretação dos resultados experimentais. / A new arrangement of measure was developed in this study, in order to obtain the energy distribution from the emitted secondaries and the emission yields by a solid. Because this arrangement allowed the direct measurement of the surface potential, in the case of metals, it was possible to apply and describe theoretically the mensuration method employed, to determine the energy distribution of the secondaries to platinum, Teflon-FEP. Mylar, PVDF α, PVDF- β and thie conducting polymer. The charges accumulated during the polymers irradiation were compensated through the electronic beam itself. This proceeding is not very usual, as referring to the ones applied in literature, and it proved to be very efficacious. The dynamic method was employed for the first time to metals in order to determine the total emission yield in function of the energy of the inciding electrons and it was found out that these results were identical to the ones obtained through the pulse method. As a natural development, by applying the dynamic method to metals, the measurements achieved were also applied to golden layers 50 nm, 10 nm and 2,5 nm wide deposited on Teflon-FEP, and they gave birth to the two layers, in the interpretation of the experimental results.

Neste trabalho estudou-se um novo arranjo de medida para se obter a distribuição de energia dos elétrons secundários emitidos e os coeficientes de emissão por um sólido. Pelo fato deste arranjo permitir a medida direta do potencial de superfície no caso dos polímeros e da ddp de contato no caso dos metais, foi possível aplicar e descrever teoricamente o método de medida empregado para determinar o espectro energético dos secundários para a platina, Teflon-FEP, Mylar, PVDF- α, PVDF- β e polimero condutor. As cargas acumuladas durante a irradiação dos polímeros foram compensadas através do próprio feixe eletrônico. Este procedimento não é muito usual em relação aqueles aplicados na literatura e mostrou-se bastante eficaz. Pela primeira vez empregou-se o método dinâmico para determinar o coeficiente de emissão total em função da energia dos elétrons incidentes nos metais e verificou-se que estes resultados são idênticos aqueles obtidos pelo método do pulso. Um desdobramento natural ao aplicar o método dinâmico aos metais foram as medidas realizadas para camadas de ouro com espessura de 50 nm, 10 nm e 2,5 nm depositada sobre o Teflon-FEP, que deram origem ao modelo das duas camadas na interpretação dos resultados experimentais. / A new arrangement of measure was developed in this study, in order to obtain the energy distribution from the emitted secondaries and the emission yields by a solid. Because this arrangement allowed the direct measurement of the surface potential, in the case of metals, it was possible to apply and describe theoretically the mensuration method employed, to determine the energy distribution of the secondaries to platinum, Teflon-FEP. Mylar, PVDF α, PVDF- β and thie conducting polymer. The charges accumulated during the polymers irradiation were compensated through the electronic beam itself. This proceeding is not very usual, as referring to the ones applied in literature, and it proved to be very efficacious. The dynamic method was employed for the first time to metals in order to determine the total emission yield in function of the energy of the inciding electrons and it was found out that these results were identical to the ones obtained through the pulse method. As a natural development, by applying the dynamic method to metals, the measurements achieved were also applied to golden layers 50 nm, 10 nm and 2,5 nm wide deposited on Teflon-FEP, and they gave birth to the two layers, in the interpretation of the experimental results.

The thesis is concerned with the design of the shape of suction canals used for pumping in the scintillation detector. The goal is to perform analysis of the model of the detector according to the current conception and then perform an optimalisation of the suction canals as per demands to their function. In the beginning of the thesis the history of the microscope and electron microscopy is described. The next chapter is focused on a more detailed description of ESEM. The following charter is dedicated to describing the scintillation detector.The thesis recounts the kinds of liquid flow. In conclusion the programs SolidWorks and Ansys Fleunt are described. Next part of this thesis describes in detail parameters‘ settings of simulation calculation. Following chapter introduces designed changes of suction channels shape and its results are shown and described.

In this master’s thesis, a mechanical design of an in-column secondary electrons (SE) detector is presented. It is an ultravacuum compatible fibre-scintillation detector designed for use in an ultrahigh vacuum scanning electron microscope (UHV SEM). The designed in-column SE detector was manufactured and tested upon overcoming R&D challenges. The first section of this thesis deals with theoretical basis needed for understanding of functional principles of UHV SEM system and means of SE’s detection. In the second section, mechanical design of the in-column SE detector is described. The last section describes functionality of the designed detector. Effectiveness of light shielding of the detector was tested and the detective quantum efficiency was measured. Finally, images created by the designed in-column detector and an in-chamber SE detector were evaluated and compared.

The thesis deals with the study of properties of a new system for detection of true secondary and backscattered electrons in high pressure conditions of the specimen chamber of a newly built environmental scanning electron microscope AQUASEM II. Detection system contains three detectors. For the first time is introduced and analyzed the working principle of ionisation detector with electrostatic separator, which is in many experiments compared with ionisation detector of secondary electrons. Experimentally demonstrated are unique properties of this detection system, especially the ability of energy separation of detected signal electrons. For the various working conditions are also analyzed signal levels detected by the BSE YAG detector, which is designed as a part of the new detection system and which worked together with both ionisation detectors.

La structure et le processus de formation des traces latentes dans le poly (allyl diglycol carbonate), PADC, ont été étudiés par spectroscopie FT-IR et par simulation Monte Carlo. La quantité de groupes OH formés est équivalente à la quantité de disparition des groupes éther. L’utilisation de radiations à faible TLE a prouvé que les fonctions carbonyle ne disparaissent que lorsque deux électrons au minimum interagissent avec une seule unité de répétition du polymère. Les résultats obtenus avec des protons de haute énergie permettent de comprendre la différence entre des traces révélables et non-révélables. Sur la base de ces résultats, un nouveau concept physique de Fluence Electronique Radiale autour de la Trace d’un Ion, défini comme la densité d'électrons secondaires qui traversent une surface cylindrique de rayon donné, est proposé pour décrire le seuil de détection du PADC en utilisant le code Geant4-DNA. Les connaissances acquises sont utiles pour trouver des agencements moléculaires appropriés pour de nouveaux détecteurs de sensibilités désirées. / The structure and formation process of latent tracks in poly(allyl diglycol carbonate), PADC, have been examined using the combination of FT-IR spectrometry and a Monte Carlo simulation. The generation amount of OH groups is almost equivalent to the loss amount of ether. An important role of the secondary electron that the carbonyl can be broken only when more than two electrons pass through a single repeat unit is clarified by experiments using low LET radiations. Results of high energy protons lead us to the elucidation of the difference between etchable and un-etchable tracks. Based on these results, a new physical concept of Radial Electron Fluence around Ion Tracks, which is defined as the number density of secondary electron that pass through the cylinder surface with a certain radius is proposed for the detection threshold of PADC using Geant4-DNA. Obtained knowledge is helpful to find appropriate molecule arrangements for new etched track detector with desired sensitivities

Lors d’un examen ou radiologique, des rayonnements X de basse énergie sont utilisés (<100 keV). Pour certains traitements radiologiques, l’énergie utilisée est de plusieurs MeV. La publication 103 de la CIPR considère actuellement que les photons, indépendamment de leur énergie, ont le même facteur de pondération. Cependant, il existe des différences topologiques à l'échelle nanométrique du dépôt d'énergie des rayonnements X en fonction de leur spectre énergétique. En effet, à mesure que l’énergie des photons décroit, la nature de leurs interactions avec la matière vivante se modifie. Pour étudier ces différences, nous avons caractérisé nos conditions d'irradiation en termes d'énergies initiales des photons, mais surtout en termes de spectres d'énergie des électrons secondaires au niveau du volume cellulaire, en utilisant des simulations de Monte Carlo. Nous nous sommes intéressés à la signalisation des dommages de l'ADN en analysant un grand nombre de foyers γH2A.X après exposition de cellules endothéliales humaines synchronisées en phase G0/G1 à des doses allant de 0,25 à 5 Gy à 40 kV, 220 kV et 4 MV. Le nombre et la distribution spatiale des foyers γH2A.X ont été explorés. Aussi, nous avons étudié la fréquence de division et de mort cellulaire. Nous avons également étudié le taux d’anomalies de ségrégation après la division cellulaire. Nous avons mis en évidence un nombre plus élevé de cassures double-brin de l'ADN signalisées par γH2A.X pour 40 kVp et/ou 220 kVp, comparé à 4 MVp pour les plus fortes doses testées de 2 et 5 Gy. Entre 40 et 220 kVp, aucune différence biologique n’a été observée. Ce manque de différence pourrait s’expliquer par la grande similarité des spectres énergétiques des électrons secondaires, au niveau du volume cellulaire.Le spectre d'énergie des électrons secondaires semble être plus étroitement lié au niveau de dommage à l'ADN mesuré par γH2A.X que le spectre initial des paramètres d'énergie ou de tension des photons. Nos résultats indiquent qu'à mesure que le spectre d'énergie des électrons secondaires augmente, les dommages à l'ADN signalés par γH2A.X diminuent et cet effet est observable au-delà de 220 kVp. / In a radiological examination, low-energy X-radiation is used (<100 keV). For other radiological procedures, the energy used is several MeV. ICRP in publication 103 has currently considered that photons irrespective of their energy have the same radiation weighting factor. Nevertheless, there are topological differences at the nanoscale of X-ray energy deposition as a function of its energy spectrum, meaning that the different interactions with living matter could vary in biological efficacy. To study these differences, we characterized our irradiation conditions in terms of initial photon energies, but especially in terms of energy spectra of secondary electrons at the cell nucleus level, using Monte Carlo simulations. We evaluated signaling of DNA damage by monitoring a large number of γH2A.X foci after exposure of G0/G1-phase synchronized human primary endothelial cells at a dose from 0.25 to 5 Gy at 40 kV, 220 kV and 4 MV X-rays. Number and spatial distribution of γH2A.X foci were explored. In parallel, we investigated cell behavior through cell death and ability of a mother cell to produce two daughter cells. We also studied the missegregation rate after cell division. We report a higher number of DNA double-strand breaks signaled by γH2A.X for 40 kVp and/or 220 kVp compared to 4 MVp for the highest tested doses of 2 and 5 Gy. We observed no difference between the biological endpoint studies with 40 kVp and 220 kVp X-ray spectra. This lack of difference could be explained by the relative similarity of the calculated energy spectra of secondary electrons at the cell monolayer. The energy spectrum of secondary electrons seems to be more closely related to the level of DNA damage measured by γH2A.X than the initial spectrum of photon energy or voltage settings. Our results indicate that as the energy spectrum of secondary electrons increases, the DNA damage signaled by γH2A.X decreases and this effect is observable beyond 220 kVp.

The diploma thesis deals with the determination of the structure of AHSS steels and offers solutions in the form of new observation methods with the aid of a scanning electron microscope using filtration of slow secondary electrons. The thesis describes electron filtration in order to display secondary electrons in a certain energy range which carry a surface information that is not normally visible. The advantages and benefits of such observation are clearly demonstrated.

This thesis is focused on the environmental scanning electron microscopes including a description of the basic physical mechanisms. It describes the design and realization of the electrode ionization detector system suitable for the detection of signal electrons with a greater proportion of secondary electrons. It also deals with simulations of trajectories of electrons in the electrostatic field of the detector, and with verification of the functionality of the detector in the environment of water vapors in the specimen chamber of the electron microscope.