This thesis deals with the association analysis of selected polymorphism of the SERPINE1 gene in a group of Czech Large White pigs. The surveyed traits were average daily gain, back fat thickness and lean meat content. The SERPINE1 gene belongs to the SERPIN family of proteins is a candidate gene for fatness and carcass traits of pig. The trial was conducted in 96. Methods of PCR and RFLP were used to detect single nucleotide polymorphism. The allele and genotype frequencies were calculated and results of genotyping were entered into a database and statistically analyzed using the SAS software. It was found that animals with AA genotype occurred most often with the frequency of 47.9%, followed by AG genotype with the frequency 40.6%. Least frequented genotype was GG (11.5%). No statistically significant differences between genotypes for above traits were found in our group of the Czech Large White pigs.