Traditionnellement, le droit prend soin de distinguer entre les clauses, parties d’un acte juridique, et les pratiques qui sont des faits juridiques. Pourtant, le droit des pratiques restrictives de concurrence semble réaliser une assimilation de ces deux notions, en qualifiant de pratiques restrictives de concurrence certaines clauses. L’étude analyse cette qualification d’une clause en pratique en proposant un critère et en s’interrogeant sur ses effets : la possible contestation de clauses sur le fondement du droit des pratiques restrictives de concurrence. En invitant à une réflexion sur cet aspect du droit des pratiques restrictives de concurrence, il s’agit également de s’interroger sur les finalités de cette matière, autant que sur la possibilité d’une meilleure intégration de la contestation de clauses dans un droit des pratiques commerciales déloyales entre professionnels en construction. / Generally, the law is careful to distinguish between the clauses, part of a legal act, and practices, that are legal facts. Yet, the law about restrictive competition practices seems to realise an assimilation of these two concepts, and provide the classification of a clause in restrictive competitive practice. The study analyses the classification of a clause into practice by proposing a criterion and pondering its effects: possible challenge clauses on the bases of the right of restrictive practices. By inviting a reflection on this aspect of the law of restrictive practices, it is also to question the aims of this particular law, as well as on the possibility of a better integration of the challenge clauses in a law about unfair trade practices between professionals in construction.

Submitted by Maria Bernadete Dos Santos (mariabpds@id.uff.br) on 2017-09-27T17:53:27Z No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertação Alexandre L. Torres.pdf: 2113771 bytes, checksum: e92b2eb0221ae3372965ba2accb94702 (MD5) Produto Final - Alexandre L. Torres.pdf: 357939 bytes, checksum: 0c6eccc1f4a85d82d3cd61292b293898 (MD5) / Approved for entry into archive by Biblioteca Central do Valonguinho Biblioteca Central do Valonguinho (bcv@ndc.uff.br) on 2017-10-03T17:14:22Z (GMT) No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertação Alexandre L. Torres.pdf: 2113771 bytes, checksum: e92b2eb0221ae3372965ba2accb94702 (MD5) Produto Final - Alexandre L. Torres.pdf: 357939 bytes, checksum: 0c6eccc1f4a85d82d3cd61292b293898 (MD5) / Made available in DSpace on 2017-10-03T17:14:22Z (GMT). No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertação Alexandre L. Torres.pdf: 2113771 bytes, checksum: e92b2eb0221ae3372965ba2accb94702 (MD5) Produto Final - Alexandre L. Torres.pdf: 357939 bytes, checksum: 0c6eccc1f4a85d82d3cd61292b293898 (MD5) / Este trabalho relata uma metodologia ativa para o Ensino de Ligações Químicas: Composição de paródias musicais como uma proposta de potencializar a aprendizagem significativa. Trata-se de uma pesquisa de natureza qualitativa, do tipo pesquisa-ação, e utilizou, para o levantamento de dados e para a tomada de decisões no decorrer do processo de pesquisa, questionários, observações da atividade orientada, depoimento dos educandos durante e após a elaboração das paródias musicais, fotos, vídeos e análise das letras das paródias. A pesquisa foi realizada no Colégio Pedro II, Campus Niterói, Rio de Janeiro e aplicada no 1º. ano do Ensino Médio Regular do ano letivo de 2016. Os resultados obtidos indicam que a construção de paródias musicais favoreceu o processo de ensino-aprendizagem, sendo esse recurso didáticos visto pelos alunos como alternativa para facilitar a aprendizagem. Consideramos que este trabalho pode ser replicado, abrindo possibilidades de novas criações e pesquisas sobre o emprego de estratégias de metodologias ativas no ensino de Ciências da Natureza. Com o intuito de incentivar esta aplicação, elaboramos, como produto dessa dissertação, uma videoaula onde é mostrada a aplicação da metodologia e com orientações sobre a forma de utilização. / This paper reports an active methodology for the Teaching of Chemical Bonds: Composition of musical parodies as a proposal to potentiate meaningful learning. For this purpose we used a qualitative methodology and, for data collection and decision making during the research process, questionnaires, observations of the activity oriented, testimony of the students during and after the elaboration of the musical parodies, photos, videos and analysis of the letters of the parodies. The research was carried out at Pedro II School, Niterói, Campus, Rio de Janeiro and applied in the 1st. Year of Regular High School during the academic year of 2106. The results indicate that the construction of musical parodies favors facilitate the teaching-learning process. We consider that this work can be replicated and open possibilities of new creations and research on the use of active methodologies for teaching of Natural Sciences. In order to stimulate this application, we elaborate, as a product of this dissertation, a videoconference showing the application of the methodology and guidelines on how to use it.

Made available in DSpace on 2016-04-29T14:17:58Z (GMT). No. of bitstreams: 1 Elisete Maria Ribeiro.pdf: 7853414 bytes, checksum: ca9d4beb14aec1e3814dc40e05452d22 (MD5) Previous issue date: 2009-11-27 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The field of health policy has incorporated new concepts that guide the qualification and practice of health professionals, following a trend of ensuring universal health care rights. Health services have required health professionals to adopt distinguished conducts in which they play a central role in delivering technical, operational and political excellence in their practices. This is because they are jointly responsible for ensuring an accessible, humanized, egalitarian and comprehensive health care, among other principles respected by the Brazilian Health System (SUS). This research sought to analyze, from a historic and institutional perspective, the service infrastructure quality, as well as Primary Health Care workers concepts and practices underpinning the implementation and the development of the Family Health Strategy in the Health Office of Curitiba. In order to meet such demand, a new professional profile emerges with a humanized approach, as well as generalist and resolute characteristics within the Family Health Strategy practice. Based on the theories raised by the attributes described by Starfield (2002) as critical or unique in Primary Health Care (first contact, comprehensiveness, longitudinality and coordination), the secondary attributes (family-centeredness, cultural competence and community orientation) and an attribute not only assigned to Primary Health Care (proper recording), the concepts and practices adopted by the Family Health Strategy in Curitiba are analyzed. Family Health was wholly taken and implemented within a specific technical and political timeframe and in a restricted fashion. It is defended only by a portion of significant professionals aligned to understand and embrace values, principles and attributes of Primary Health Care. Significant individuals demonstrate the progresses made in the qualification of working concepts and processes and the contradictions arising from an insufficient appropriation of ideologies by new stakeholders, which impair the delivery of a qualified Family Health Care work. This study presents how the Primary Health Care in Curitiba is organized and operates, in connection with the hegemony and qualified development of a city policy / O campo da política de saúde tem agregado novas concepções que orientam a formação e a prática dos trabalhadores de saúde, em uma tendência de garantia da universalidade do direito à saúde. O trabalho em saúde tem exigido condutas diferenciadas de seus trabalhadores, na condição de protagonistas da excelência técnica, operativa e política de sua prática profissional, por se constituírem como sujeitos corresponsáveis na garantia de acesso, humanização, equidade e integralidade da atenção em saúde, entre outros princípios do SUS. A pesquisa buscou analisar, no processo histórico-institucional, a qualificação da infraestrutura de serviços, bem como as concepções e práticas dos trabalhadores em APS que fundamentam a implantação e o desenvolvimento da estratégia Saúde da Família- SF, na SMS Curitiba. Para responder a tal demanda, demonstra-se a constituição de um novo perfil profissional, de abordagem humanizada, com características mais generalistas e resolutivas da pratica profissional em ESF. Com a base teórica no campo dos atributos descritos por Starfield (2002) como essenciais ou únicos em APS (primeiro contato, integralidade, longitudinalidade e coordenação), os atributos derivados (enfoque na família, valorização dos aspectos culturais e orientação na comunidade) e um atributo não exclusivo da APS (registro adequado), fundamenta-se a análise das concepções e práticas das ESF em Curitiba. Em sua integralidade, a Saúde da Família foi apreendida e implementada em tempo técnico e político especifico, de forma restrita, defendida por apenas um segmento de profissionais significativos, alinhados em compreensão e defesa de uma base de valores, princípios e atributos da APS. Assim, os sujeitos significativos demonstram os avanços na qualificação das concepções e processo de trabalho, e da mesma forma as contradições evidenciadas por uma insuficiente apropriação de bases ideológicas e adesão por parte de novos protagonistas que comprometem a qualificação do trabalho em Saúde da Família. Demonstra-se a capacidade de organização e funcionamento da APS em Curitiba, relacionada à hegemonia e desenvolvimento qualificado de uma política municipal

We share the results from a year-long randomized control trial study targeting a comprehensive approach to teach grade aligned ELA via an App for middle school students with significant disabilities. Participants will learn about the embedded evidence-based practices that led to significant outcomes.

<p>The aim of this degree project has been to examine, analyze and describe which intra-linguistic factors influence how relative clauses are formed in Old English.</p><p>The key to successfully performing the task of identifying which factors influences the relative causes is to examine how these factors are distributed among the relative clauses in the text. The main focus of this investigation thus was to investigate how the grammatical features of the antecedents of the relative clauses in Old English were distributed. By analyzing a text sample of the work of the Old English writer Ælfric, taken from the Dictionary of Old English Corpus at the University of Toronto, also known as the Toronto Corpus, several features of the antecedent will ideally become evident as influencing factors.</p><p>The relative clauses that are found to be relevant for this investigation in the Ælfric text sample have been categorized and analyzed in order to identify any grammatical pattern that could indicate which factors influence how relative clauses in Old English are formed. The findings have been analyzed according to quantitative and statistical principles, and the chi-square test has been employed to verify the statistical significance of these findings. By doing this some linguistic factors have been verified as influencing factors.</p>

MPEG audio compression techniques significantly reduces the storage and transmission requirements for high quality digital audio. However, compression complicates the processing of audio in many applications. If a compressed audio signal is to be processed, a direct method would be to decode the compressed signal, process the decoded signal and re-encode it. This is computationally expensive due to the complexity of the MPEG filter bank. This thesis deals with processing of MPEG compressed audio. The main contributions of this thesis are a) Extracting wavelet coefficients in the MPEG compressed domain. b) Wavelet based pitch extraction in MPEG compressed domain. c) Time Scale Modifications of MPEG audio. d) Watermarking of MPEG audio. The research contributions starts with a technique for calculating several levels of wavelet coefficients from the output of the MPEG analysis filter bank. The technique exploits the toeplitz structure which arises when the MPEG and wavelet filter banks are represented in a matrix form, The computational complexity for extracting several levels of wavelet coefficients after decoding the compressed signal and directly from the output of the MPEG analysis filter bank are compared. The proposed technique is found to be computationally efficient for extracting higher levels of wavelet coefficients. Extracting pitch in the compressed domain becomes essential when large multimedia databases need to be indexed. For example one may be interested in listening to a particular speaker or to listen to male female audio segments in a multimedia document. For this application, pitch information is one of the very basic and important features required. Pitch is basically the time interval between two successive glottal closures. Glottal closures are accompanied by sharp transients in the speech signal which in turn gives rise to a local maxima in the wavelet coefficients. Pitch can be calculated by finding the time interval between two successive maxima in the wavelet coefficients. It is shown that the computational complexity for extracting pitch in the compressed domain is less than 7% of the uncompressed domain processing. An algorithm for extracting pitch in the compressed domain is proposed. The result of this algorithm for synthetic signals, and utterances of words by male/female is reported. In a number of important applications, one needs to modify an audio signal to render it more useful than its original. Typical applications include changing the time evolution of an audio signal (increase or decrease the rate of articulation of a speaker),or to adapt a given audio sequence to a given video sequence. In this thesis, time scale modifications are obtained in the subband domain such that when the modified subband signals are given to the MPEG synthesis filter bank, the desired time scale modification of the decoded signal is achieved. This is done by making use of sinusoidal modeling [I]. Here, each of the subband signal is modeled in terms of parameters such as amplitude phase and frequencies and are subsequently synthesised by using these parameters with Ls = k La where Ls is the length of the synthesis window , k is the time scale factor and La is the length of the analysis window. As the PCM version of the time scaled signal is not available, psychoacoustic model based bit allocation cannot be used. Hence a new bit allocation is done by using a subband coding algorithm. This method has been satisfactorily tested for time scale expansion and compression of speech and music signals. The recent growth of multimedia systems has increased the need for protecting digital media. Digital watermarking has been proposed as a method for protecting digital documents. The watermark needs to be added to the signal in such a way that it does not cause audible distortions. However the idea behind the lossy MPEC encoders is to remove or make insignificant those portions of the signal which does not affect human hearing. This renders the watermark insignificant and hence proving ownership of the signal becomes difficult when an audio signal is compressed. The existing compressed domain methods merely change the bits or the scale factors according to a key. Though simple, these methods are not robust to attacks. Further these methods require original signal to be available in the verification process. In this thesis we propose a watermarking method based on spread spectrum technique which does not require original signal during the verification process. It is also shown to be more robust than the existing methods. In our method the watermark is spread across many subband samples. Here two factors need to be considered, a) the watermark is to be embedded only in those subbands which will make the addition of the noise inaudible. b) The watermark should be added to those subbands which has sufficient bit allocation so that the watermark does not become insignificant due to lack of bit allocation. Embedding the watermark in the lower subbands would cause distortion and in the higher subbands would prove futile as the bit allocation in these subbands are practically zero. Considering a11 these factors, one can introduce noise to samples across many frames corresponding to subbands 4 to 8. In the verification process, it is sufficient to have the key/code and the possibly attacked signal. This method has been satisfactorily tested for robustness to scalefactor, LSB change and MPEG decoding and re-encoding.

本研究旨在探討校長工作價值觀、重要他人支持、自我效能感對其工作選擇的影響情形，探討不同重要變項理論內涵、現況與變項之間關係，進而建構及驗證其互動模式，並依研究結果提出建議。 本研究先進行文獻探討，作為架構研究的理論基礎，並以100名校長為預試樣本，分析預試結果以形成正式問卷，再以404名之校長為正式問卷施測樣本，問卷回收整理後分別以描述統計、t考驗、相關分析、變異數分析、多元逐步迴歸分析及線性結構模式分析探討；之後，根據調查結果研擬訪談題綱，實地訪談10名現職校長以瞭解造成差異的原因。本研究之主要研究發現如下： 一、國民中小學校長在工作價值觀、重要他人支持、自我效能感與工作選擇的得分現況屬於中高程度。 二、擔任校長的年資、性別、學校區域等背景變項，在校長工作價值觀量表的得分有差異情形存在。 三、學校類別、總服務年資、擔任校長的年資、性別、學校區域等背景變項，在重要他人支持量表的得分有差異情形存在。 四、總服務年資、擔任校長的年資、性別、學校區域等背景變項，在校長自我效能感量表的得分有差異情形存在。 五、學校類別、擔任校長的年資、性別、學校區域等背景變項，在校長工作選擇量表的得分有差異情形存在。 六、工作價值觀、重要他人支持、自我效能感與校長工作選擇具高度相關。 七、工作價值觀、重要他人支持、自我效能感對校長工作選擇有顯著預測力。 八、工作價值觀、重要他人支持、自我效能感對校長工作選擇有顯著影響力。 九、校長自我效能感對其工作選擇的影響力最大，重要他人支持次之，工作價值觀的影響力最小。 最後，根據研究結果提出相關建議，以供教育行政機關、校長培育中心、現職校長以及未來研究之參考。 / The purpose of this study was to investigate the impact of work values, significant other’s support, and self-efficacy for principal’s job choice. The study included literature analysis, questionnaire survey, and interview. The purpose of literature analysis was aimed to explore the relationship of work values, significant other support, self-efficacy, and job choice. The pilot study was included 100 subjects. The sample was included 404 principals. The data was analyzed by descriptive statistics, t-test, correlation, ANOVA, multiple regression and LISREL model. The interview was conducted according to the analyzed results. The major findings were: 1.The principal’s scores on work value, significant other’s support, self-efficacy and job choice were moderately high. 2.Years of principalship, gender, and districts could make significant differences on principal’s work values 3.The levels of schools, in-service years, years of principalship, gender, and districts could make differences on significant other’s support. 4.In-service years, years of principalship, gender, and districts could make differences on principal’s self-efficacy. 5.The level of schools, years of principalship, gender, and districts could make differences on principal’s job choice. 6.There was highly positive correlation among principal’s work values, significant other’s support, self-efficacy, and job choice 7.The principal’s job choice could be predicted by work values, significant other’s support, self-efficacy. 8.The principal’s job choice could be affected by work values, significant other’s support, self-efficacy. 9.Principal’s self-efficacy was the strongest indicator for job choice, significant other’s support was the second one, and work values were the smallest one. According to the results, suggestions for authorities of educational administration, School leadership academy, principal, and for further study were proposed.

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal

Bakgrunn og hensikt: For mennesker som blir akutt/kritisk syk blir livet oftest endret på kort tid fra å værevelfungerende i hverdagen til innleggelse i intensivavdeling hvor selveste livet kan stå på spill. Tradisjonelt settblir intensivpasienter tatt vare på gjennom medisinsk kunnskap og et naturvitenskapelig kunnskapsparadigme. Åvære akutt/kritisk syk innebærer å få spesielle opplevelser og erfaringer som går ut over hverdagserfaringene, oghvordan disse erfaringene trer fram i situasjonen. Denne kunnskapen pasientene bærer i seg er ikke bareindividuell og privat kunnskap, den representerer også en form for viten som trenger å forskes fram og løftes inni det offentlige rom både folkehelsevitenskapelig og sykepleievitenskaplig, nettopp fordi den omhandler viktigeforutsetninger for at pasientene skal bli sett og møtt også eksistensielt. Denne kunnskapen representerer en annenontologi og epistemologi enn den naturvitenskapelige kunnskapen og representerer derfor et annetkunnskapsperspektiv, også når det gjelder kunnskapsformidlingen i en klinisk veiledningssammenheng. Avhandlingens overordnede mål er å øke forståelsen av det å være alvorlig syk og hvordan kunnskap ut fraerfaringer har betydning i kunnskapsformidling i en intensivkontekst. Data og metoder: Avhandlingen har en kvalitativ tilnærming. I delstudie I ble det gjort en review avartikler for å se på stress relatert til kropp, rom og relasjon. I delstudie II ble det gjennomført intervju medtidligere intensivpasienter om opplevelsen av kropp, kraft og bevegelse under kritisk sykdom. I delstudie III bletidligere intensivpasienter intervjuet angående deres opplevelse av kroppsnærværet til pårørende under kritisksykdom. I delstudie IV ble det gjort observasjoner med påfølgende intervjuer av intensivsykepleiere om deresformidling av erfart og fortolket kunnskap i en klinisk veiledningssituasjon. I alle fire delstudiene benyttes detfenomenologisk-hermeneutiske analyser. Funn: I delstudie I kommer det fram at pasientene opplever stress knyttet til kropp, rom og relasjon.Kroppen påvirkes gjennom søvnavbrudd, smerter, angst og pasientene mister kontrollen over kropp og situasjon.Stress knyttet til rom framkommer gjennom at rommet blir et speil av situasjonen ved at den både representererlivet, men også redselen for døden. Gjennom det horisontale leie opplever pasientene at situasjonen utgjør enform for makt og de selv settes i avmakt. Relasjonelt stress framkommer oftest knyttet til sammenhenger hvordet utføres observasjoner, stell og behandling. Travelhet og organisering i avdelingen påvirker det relasjonellesamværet i form av stress. I delstudie II opplever intensivpasientene at kropp kraft og bevegelse trer fram bådegjennom ”tapet” av kroppen, hvordan de handterer situasjonen og hvordan de gjenerobrer kropp, kraft ogbevegelse. De opplever kraftløshet og bevegelsesbergrensninger. Dette innvirker på avhengigheten til andre ogskaper konflikter til pårørende. Pasientene handterer det kroppslige tapet gjennom mobilisering av familiengjennom å involvere dem. Samtidig blir de selvbeskyttende og utestenger familiesammenhengene. Kampen forlivet håndterer de gjennom galgenhumoren og skriket om hjelp som siste nødrop. Gjenerobringen av kroppen erknyttet til små framskritt, gjennom personalets motivasjon og gjennom drømmen om å utrette noe i livet. Idelstudie III opplever pasientene konflikt mellom nærheten og distanse til pårørende. De kjenner seg utestengt,samtidig som de bekreftes med gaver. Pasientene opplever det konfliktfylt når de sammenligner pårørendesreaksjoner og egen situasjon. De er redd at fellesskapet med pårørende skal opphøre, samtidig må de begrensesamværet. Å ikke kunne kommunisere med pårørende i kroppsnærværet oppleves motsetningsfullt. I delstudieIV formidler sykepleierne kunnskap til intensivstudentene gjennom meningsskapende kunnskapsbevegelser. Deformidler inntrykk fra situasjonen for å skape oversikt, de formidler kunnskap om fenomenene som framtrer ogde formidler hvordan de kan hjelpe kroppens egne prosesser. De vurderer og formidler også pasienteneskroppslige uttrykk og hvordan disse kan forstås og imøtekommes. Sykepleierne er også opptatt å formidlekunnskap for at studentene skal utvikle og få egne erfaringer i kroppen. Konklusjon: Gjennom fire delstudier løfter avhandlingen fram kunnskap som står i klar kontrast til dennaturvitenskapelige kunnskapen. Pasientene utsettes for store opplevelsesmessige belastninger i situasjonen somakutt/kritisk syke som setter de i en avmaktssituasjon ved at sykepleierne ikke fanger opp og handterer kroppensegen kunnskap i situasjonen. Pasientene befinner seg ofte i en eksistensiell situasjon som er preget avmarginalitet og eksistensiell væren. Pasientene viser at de har forutsetninger for å mestre situasjonen, menhjelpes lite til slik mestring. Intensivsykepleierne formidler ulikt faglig innhold til studentene for å skapeerfaringer og forståelse i situasjonen. Erfaringskunnskapen fra intensivkonteksten må derfor sees som et viktigkunnskapssupplement til folkehelsearbeidet. / Background and purpose: Acute/critically ill patients have had their life-conditions severely altered from awell-functioning everyday situation to a situation in which they struggle to survive. Traditionally the knowledgeparadigmfrom natural sciences together with medical expertise constitutes the framework for treatment. To beacute/critically ill involves experiences and events and phenomena typical to the situation. The patients’embodied knowledge is not limited to the personal and private sphere, it refers to highly relevant preconditionsfor visibility and reception, also on an existential level; it represents knowledge which needs to be researchedand discussed openly within the public health and nursing sciences. This knowledge represents a differentontology and epistemology from the natural sciences and thus a different perspective, also with regards toclinical tutoring. The dissertation’s primary target is to increase the knowledge about how critical illness isexperienced and how experience based knowledge is significant to teaching in an intensive care context. Data and methods: The dissertation is based on qualitative methods. Sub-study I includes reviews ofarticles on stress in a perspective of body, space and relationship. Sub-study II contains interviews with formerICU patients relating to their experience of body, strength and movement during critical illness. Sub-study III isbased on former ICU patients’ experience of the physical presence of their significant others during criticalillness. Sub-study IV contains observations and interviews of ICU nurses on how they teach experience basedand interpretation based knowledge in clinical tutoring. Phenomenological-hermeneutic analysis method isapplied to all four sub-studies. Findings: Sub-study I describes how patients experience stress in a perspective of body, space andrelation. The body is influenced by sleep deprivation, pain and anxiety. Patients experience loss of control withtheir bodies as well as with the situation. The room mirrors the situation patients are in, creating stress by itsrepresentation of life as well as fear of dying. The patient’s horizontal position reflects disproportionate powerinducing a sense of disempowerment. Relational stress situations usually appear in contexts involvingobservation, care or treatment. The organizational structure and activity on the ward affects stress levels. In substudyII the ICU patients experience how power and movement is affected by a sense of loss of body, how theymanage to respond to the situation and how they eventually reclaim the body, power and movement. Theyexperience disempowerment and limitations to movement. This causes dependency and creates conflicts withtheir significant others, especially with regards to proximity and distance. The patients address their loss of bodyby drawing on resources from their significant others, yet refrain from allowing themselves involvement infamily matters. The struggle for survival is characterised by gallows humour and the cry for help as their finalcry. Reclaiming the body happens stepwise with the aid of staff combined with a need to achieve. Sub-study IIIdescribes problems related to proximity to, and distance from, the significant others. A sense of exclusion iscontradicted by being showered with gifts. It is unsettling for patients to have their situation compared to theirsignificant others’. They fear the loss of community with loved ones, yet they need to limit visits fromsignificant others. The lack of ability to communicate when others are physically present is unsettling. Sub-studyIV describes how ICU nurses tutor students in practise by demonstrating knowledge in meaningful actions. Theyconvey their impressions of situations to provide an overview, convey knowledge of observable phenomena, andteach skills to assist the body’s own processes. They also assess and evaluate physical expressions in the patientand how these can be read and addressed. Nurses are also concerned with conveying embodied knowledge totheir students. Conclusion: In four sub-studies this dissertation highlights a form of knowledge which clearly contraststhat of the natural sciences. During acute critical illness patients experience severe stress and disempowermentcaused by a lack of response and action from nurses who are unable to interpret the body’s own knowledge inthe situation. Patients are often facing life and death questions characterised by marginality and existentiality.Patients demonstrate ability to cope with the situation, but are rarely assisted with coping. Intensive care nursesdisseminate a variety of professional skills and knowledge to their students in order to provide comprehensionand understanding of situations. Experience based knowledge from an intensive care context thus becomes asignificant supplementary factor in the public health perspective

The freshwater fish fauna of southern Australia is characterised by low species richness and high endemism in groups displaying southern temperate, temperate-subtropical or temperate-tropical distributions. Comparatively few studies in Australia have incorporated modern molecular techniques to delineate species boundaries and define within-species conservation units. This is problematic because freshwater fishes are likely to show high levels of cryptic speciation and marked spatial sub-structure, and is information which is needed to conserve biological diversity and maintain the integrity of ecological communities and processes. The current study uses a ‘combined evidence’ approach, led principally by a set of nuclear genetic markers (allozymes), to assess species boundaries, spatial sub-structure and conservation units in obligate freshwater fishes from southern Australia. A literature review (Chapter 2) concerns the nature and effects of fragmentation in freshwater environments. It considers the implications for freshwater fishes and the types of extrinsic and intrinsic characteristics, both natural and human accelerated, that might drive population fragmentation and divergence. This theoretical framework is then applied to a suite of six largely co-occurring species groups with contrasting biological characteristics, and derive hypotheses about expected levels of genetic divergence across and within different drainages. Major findings Species of Retropinna (Chapter 3) are widespread and generally regarded as ‘common’ and mobile. Allozyme analyses revealed species-level and population-level sub-divisions, including five distinct species with contiguous ranges and no evidence of genetic exchange. Three occur along the eastern seaboard (including three instances of sympatry), another in coastal and inland southeastern Australia and Tasmania, and a fifth in the Lake Eyre Basin. There is no indication of a simple ‘tasmanica’ versus ‘semoni’ dichotomy, but instead a complex pattern involving discrete clusters for the Upper Murray plus Darling rivers, Lower Murray, Glenelg River and Tasmanian regions. These findings have implications for biodiversity, conservation and ecology. This chapter has been published in modified form (Marine and Freshwater Research 58, 327- 341). Nannoperca obscura (Chapter 4) is a small demersal fish with specialised habitat requirements. It is under threat of extinction, particularly in the western section of its range. Combined nuclear and matrilineal genetic data identified congruent within-species sub-structure, divided by patternsof distribution and biogeography. Four monophyletic mtDNA lineages, each distinct at multiple nuclear loci, indicate four Evolutionarily Significant Units (ESUs), namely (1) Lake Alexandrina in the Murray-Darling Basin (MDB), (2) Glenelg River, Millicent Coast River Basin and the outlying Mt Emu Creek, (3) Merri River and associated coastal streams, and (4) the eastern range section. Additional genetic and ecological data support multiple Management Units (MUs) within ESUs for individual or groups of river basins separated by marine barriers. Nannoperca australis (Chapter 5) has a similar character to its aforementioned congener, except that it occurs across a much wider area. Although generally common, particular populations are threatened, especially in the MDB. Allozyme analyses of 57 populations confirm the presence of two divergent species, with an eastern species containing two ESUs: (1) Gippsland and Flinders Island, and (2) Ansons River in northeastern Tasmania. The western species shows sub-structure across its range, including a separation of MDB and coastal populations as two heterogenous ESUs. The Lower Murray region (Mount Lofty Range streams and the Lower Lakes) harbours a remarkable level of between- and within-population diversity, underscoring its importance for conserving evolutionary potential. Mogurnda adspersa (Chapter 6) has been presumed extinct in South Australia since the early 1970s and has also been assumed lost from the southern MDB. This chapter reports on the rediscovery of M. adspersa from a wetland near the terminus of the Lower Murray, some 2500 river kilometres from the nearest known population. The nature and basic ecology of this population is documented, but the combined effects of drought and water abstraction recently have led to the probable extirpation of the wild population. A combined allozyme and mtDNA dataset confirmed the ‘nativeness’ of the population as a distinct sub-population (and MU), with a moderate level of allele heterogeneity. This information provides a platform for captive breeding as a conservation measure. The endemic genus Philypnodon (Chapter 7) contains two nominal species: P. grandiceps and the long recognised but only recently described P. macrostomus. The former is considered widespread and common (near ubiquitous), whereas the latter is more patchily distributed. Some tolerance to marine conditions is indicated, suggesting that there may be less sub-structure, but allozyme analyses of 269 individuals indicate the presence of multiple, species-level taxa within both described species. This obscures interpretations of existing ecological data. Although the presence of genetically-similar populations within and across some drainage divides indicates higher levels of gene flow, the pattern is complex and suggests historic genetic exchange between some but not other geographically-adjacent taxa. The freshwater blackfish genus Gadopsis (Chapter 8) has been a problem group for taxonomists, and it is unclear where the group is placed phylogenetically and how many species occur. Northern and southern forms on respective sides of the Great Dividing Range have been proposed, but with limited supporting evidence. Its dispersal ability (hence predicted genetic structure) is obscured by opposing life-history traits, including large body size (i.e. good swimming ability) versus habitat specialisation, demersal larvae and restricted home ranges. This chapter provides a genetic overview incorporating 61 locations across the range, and demonstrates unequivocally the presence of distinct northern and southern species of G. ‘marmoratus’. Moreover, distinct genetic discontinuities involving geographically abutting lineages indicate the likely presence of multiple ESUs within each species. A comparison of the allozyme data with previous mtDNA studies also identified two ESUs within G. bispinosus. Overall, considerable complexity is demonstrated signalling the need for a review of how the southern Australian fish fauna should be viewed, studied and protected. The genetic data also provide insight into the interplay of intrinsic biological characters (e.g. dispersal ability, population ecology) with historic and contemporary extrinsic environmental factors (e.g. fragmentation, biogeographic processes). Comparisons between and within traditionally-defined species are problematic, however, owing to multiple species-level splits and other genetic divisions that may have matching biological counterparts. Together with other reports in the literature, the findings presented herein have significant conservation implications, particularly given the rapid pace of human-mediated change in some regions that house high species and genetic diversity and unique evolutionary components, notably southeastern Queensland (especially the Mary River) and the lower River Murray in South Australia. Other regions displaying high genetic substructure or divergent populations include the Clarence River and Lachlan River in New South Wales; Gippsland, Goulburn River, Glenelg River and Mt Emu Creek in Victoria, and the Macquarie River and Ansons River in Tasmania. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1339749 / Thesis (Ph.D.) - University of Adelaide, School of Earth and Environmental Sciences, 2008