In this thesis, we set up a framework to define knot invariants for each choice of a symmetric space. In order to address this task, we start by defining appropriate notions of singular bundles and singular connections for a given symmetric space. We can associate a moduli space to any singular bundle defined over a compact 4-manifold with possibly non-empty boundary. We study these moduli spaces and show that they enjoy nice properties. For example, in the case of the symmetric space SU(n)/SO(n) the moduli space can be perturbed to an orientable manifold. Although this manifold is not necessarily compact, we introduce a comapctification of it. We then use this moduli space for singular bundles defined over 4-manifolds of the form YxR to define knot invariants. In another direction we mimic the construction of Donaldson invariants to define polynomial invariants for closed 4-manifolds equipped with smooth action of Z/2Z. / Mathematics