This master´s thesis deals with of human factor impact on optical fibers manipulation. It describes types and methods used for splicing and placing optical fibers and optical cables. These methods are specified in the chapters; their application in different environments and conditions is accentuated. This paper presents splicing optical fibers. It emphasizes problems which can occur if proper procedures aren´t kept or if improper device is used. In the thesis there is the chapter dealing with problems which can arise during splicing optical fibers - these negative influences are evaluated with educational equipments EF-970-01 PLASTIC by the company MIKROKOM, s.r.o. Another aim of the work is to describe mechanical optical fiber splice 3MTM FibrlokTM. This kind of device was lent for testing optical fibers multiple splicing. It was tested under extreme conditions. Statistically processed values of insertion loss and splice reflectance, measured by OTDR are the results of the test. Comparing measured values, technical report ones, regarding for usage in practice, there is a possibility of 75 multiple reusing of the mechanical optical fiber splice. The prediction of magnitude insertion loss, calculated for thousands splice cycles follows. The next part of the work deals with fusion splices and contains comparison of splices made with two cleavers: CORNING LWL-TRENNGERAT S46999-M9-D12 and the later model - Fitel S325A cleaver. The result is that older S46999-M9-D12 model gained lower attenuation. The results of both previous measurements are influenced by human factor. It appears at mechanical optical fiber splices as growing fluctuate of insertion losses and as the unequal attenuation fusion splices at tested cleavers.