<p>Our knowledge of the fine structure of the Human Spiral Ganglion (HSG) is still inadequate and new treatment techniques for deafness using electric stimulation, call for further information and studies on the neuronal elements of the human cochlea. This thesis presents results of analyses of human cochlear tissue and specimens obtained during neurosurgical transpetrosal removal of life-threatening meningeomas. The use of surgical biopsies produced a well-preserved material suitable for ultrastructural and immunohistochemical studies on the human inner ear. The SG was studied with respect to fine structure, using TEM technique and the immunostaining pattern of synaptophysin, which is an integral membrane protein of neuronal synaptic vesicles. The immunostaining patterns of the tight junctional protein ZO-1 and the gap junctional proteins Cx26 and Cx43 in the human cochlea were also studied. The ultrastructural morphology revealed an absence of myelination pattern in the HSG, thus differing from that in other species. Furthermore, formation of structural units as well as signs of neural interaction between the type 1 neurons could be observed. Type 1 cells were tightly packed in clusters, sharing the ensheathment of Schwann cells. The cells frequently made direct physical contact in Schwann cell gaps in which membrane specializations appeared. These specializations consisted of symmetrically or asymmetrically distributed filamentous densities along the apposed cell membranes. The same structures were also present between individual unmyelinated nerve fibres and the type 1 cells. Synapses were observed on the type 2 neurons, with nerve fibres originating from the intraganglionic spiral bundle. Such synapses, though rare, were also observed on the type 1 cells. The ultrastructural findings were confirmed by the synaptophysin study. A 3-D model of a Schwann cell gap between two type 1 cells was constructed, describing the distribution pattern of membrane specializations. In the immunohistochemical studies on the human cochlea, ZO-1 was expressed in tissues lining scala media, thus contributing to the formation of a closed compartment system, important for the maintenance of the specific ionic composition of the endolymph. Protein Cx26 could be identified in non-sensory epithelial cells of the organ of Corti, in connective tissue cells of the spiral ligament and spiral limbus, as well as in the basal and intermediate cell layers of stria vascularis. Cx26 in this region may be involved in the recycling of potassium. Protein Cx43 stained weakly in the spiral ligament, but intense staining in the SG may indicate that gap junctions exist between these neurons. A different functional role for the HSG can be assumed from the morphological characteristics and the signs of a neural interaction between the SG cells. This might be relevant for neural processing mechanisms in speech coding and could have implications for cochlear implant techniques.</p>

Our knowledge of the fine structure of the Human Spiral Ganglion (HSG) is still inadequate and new treatment techniques for deafness using electric stimulation, call for further information and studies on the neuronal elements of the human cochlea. This thesis presents results of analyses of human cochlear tissue and specimens obtained during neurosurgical transpetrosal removal of life-threatening meningeomas. The use of surgical biopsies produced a well-preserved material suitable for ultrastructural and immunohistochemical studies on the human inner ear. The SG was studied with respect to fine structure, using TEM technique and the immunostaining pattern of synaptophysin, which is an integral membrane protein of neuronal synaptic vesicles. The immunostaining patterns of the tight junctional protein ZO-1 and the gap junctional proteins Cx26 and Cx43 in the human cochlea were also studied. The ultrastructural morphology revealed an absence of myelination pattern in the HSG, thus differing from that in other species. Furthermore, formation of structural units as well as signs of neural interaction between the type 1 neurons could be observed. Type 1 cells were tightly packed in clusters, sharing the ensheathment of Schwann cells. The cells frequently made direct physical contact in Schwann cell gaps in which membrane specializations appeared. These specializations consisted of symmetrically or asymmetrically distributed filamentous densities along the apposed cell membranes. The same structures were also present between individual unmyelinated nerve fibres and the type 1 cells. Synapses were observed on the type 2 neurons, with nerve fibres originating from the intraganglionic spiral bundle. Such synapses, though rare, were also observed on the type 1 cells. The ultrastructural findings were confirmed by the synaptophysin study. A 3-D model of a Schwann cell gap between two type 1 cells was constructed, describing the distribution pattern of membrane specializations. In the immunohistochemical studies on the human cochlea, ZO-1 was expressed in tissues lining scala media, thus contributing to the formation of a closed compartment system, important for the maintenance of the specific ionic composition of the endolymph. Protein Cx26 could be identified in non-sensory epithelial cells of the organ of Corti, in connective tissue cells of the spiral ligament and spiral limbus, as well as in the basal and intermediate cell layers of stria vascularis. Cx26 in this region may be involved in the recycling of potassium. Protein Cx43 stained weakly in the spiral ligament, but intense staining in the SG may indicate that gap junctions exist between these neurons. A different functional role for the HSG can be assumed from the morphological characteristics and the signs of a neural interaction between the SG cells. This might be relevant for neural processing mechanisms in speech coding and could have implications for cochlear implant techniques.

The aim of the Diploma Thesis is to present a German teacher profile and upcoming teacher profile in the Czech Republic, to specify basic elements of German language teacher and to analyze current profiles of College of Education graduates ? German language specializations, which are introduced by College of Education Departments of German language at universities in the Czech Republic. The theoretical part deals with current situation in Czech educational system, curriculum documents and competence issues. In reference to a presentation of standard´s proposal of the Czech Republic and standard´s proposal of Germany in the practical part, the German educational system state is also described. With regard to a conception of standard, the Thesis also deals with competence issues. The profiles of College of Education graduates are compared in the practical part, from several departments with each other and also with a German standard. Based on the PAneCZ project realized by international cooperation of Passau University and University of South Bohemia in České Budějovice, which the author of this Diploma Thesis took part in, a questionnaire analysis is made public in the practical part. This questionnaire was focused on imaginations and opinions of secondary schools´students about teaching profession. The analysis sums up what competences a primary school teacher should hold.

With the promise to shape the future of industry, multi-agent robotic technologies have the potential to change many aspects of daily life. Over the coming decade, they are expected to impact transportation systems, military applications such as reconnaissance and surveillance, search-and-rescue operations, or space missions, as well as provide support to emergency first responders. Motivated by the latest developments in the field of robotics, this thesis contributes to the evolution of the future generation of multi-agent robotic systems as they become smarter, more accurate, and diversified in terms of applications. But in order to achieve these goals, the individual agents forming cooperative robotic systems need to be specialized in what they can accomplish, while ensuring accuracy and preserving the ability to perform diverse tasks. This thesis addresses the problem of task allocation in swarm robotics in the specific context where specialized capabilities of the individual agents are considered. Based on the assumption that each individual agent possesses specialized functional capabilities and that the expected tasks, which are distributed in the surrounding environment, impose specific requirements, the proposed task allocation mechanisms are formulated in two different spaces. First, a rudimentary form of the team members’ specialization is formulated as a cooperative control problem embedded in the agents’ dynamics control space. Second, an advanced formulation of agents’ specialization is defined to estimate the individual agents’ task allocation probabilities in a dedicated specialization space, which represents the core contribution of this thesis to the advancement and practice in the area of swarm robotics. The original task allocation process formulated in the specialization space evolves through four stages of development. First, a task features recognition stage is conceptually introduced to leverage the output of a sensing layer embedded in robotic agents to drive the proposed task allocation scheme. Second, a matching scheme is developed to best match each agent’s specialized capabilities with the corresponding detected tasks. At this stage, a general binary definition of agents’ specialization serves as the basis for task-agent association. Third, the task-agent matching scheme is expanded to an innovative probabilistic specialty-based task-agent allocation framework to generalize the concept and exploit the potential of agents’ specialization consideration. Fourth, the general framework is further refined with a modulated definition of the agents’ specialization based on their mechanical, physical structure, and embedded resources. The original framework is extended and a prioritization layer is also introduced to improve the system’s response to complex tasks that are characterized based on the recognition of multiple classes. Experimental validation of the proposed specialty-based task allocation approach is conducted in simulation and on real-world experiments, and the results are presented and discussed in light of potential applications to demonstrate the effectiveness and efficiency of the proposed framework.