Corneal sensitivity and adverse events following subconjunctival administration of three local anesthetics in twelve mares were evaluated. The subconjunctival space of the treated eye was injected with 0.2 mL of mepivacaine (2%), ropivacaine (1.3%) and liposomal bupivacaine (1.3%). All horses received each medication once and the contralateral eye received saline. The corneal touch threshold (CTT) was measured using a esthesiometer before sedation, after sedation, and at specified intervals until it returned to baseline. The mean total time of anesthesia (TTA) was 168.3 minutes for ropivacaine, 169.2 minutes for liposomal bupivacaine, 103.3 minutes for mepivacaine and 30.7 minutes for control. The TTA for liposomal bupivacaine (P<0.001) and ropivacaine (P=0.001) was longer than the control. The TTA for mepivacaine was not different from the control (P = 0.138), liposomal bupivacaine (P = 0.075) or ropivacaine (P = 0.150). Injection site hemorrhage reduced TTA regardless of treatments (P=0.047). No adverse effects were noted.