Thesis contains a design of the arboretum of native woody plants of Czech Republic, which should be placed in the area of the Natural History Museum Semenec in Tyn nad Vltavou. Its aim is to introduce diversity of Czech trees and shrubs species. The arboretum is arranged so as to present particular vegetation zones. This indicator appears in terms of popular scientific mission of the arboretum as the most obvious and comprehensible. Special sections of arboretum are devoted to the azonal communities including those that develops in terms of marginal hydric series. Created arboretum of native woody plants of Czech Republic will serve to general public (tourists, school groups, univerzity students etc.) in the implementation of science educational programs