This diploma thesis elaborates on dangers of intentional misuse of transported hazardous substances. Introduction part is about legislation which determines conditions for hazardous substances treatment and their transportation. There are several types of transportation, they are described in separated chapter. Road transportation dominates in Czech Republic. Focus is put on motivation factors for misuse of hazardous substances. Factors increasing risk of misuse of hazardous substances follow. Based on it, suggestions for precautions and means to lower the risk were created. Separated chapter is dedicated to methodology for determining attractiveness of hazardous substances. To map situation in Czech Republic, I did survey in several companies. Results are summarized in last chapter.