"Esta monografía fué presentada en la Universidad nacional como tésis para el grado de doctor en derecho público."--Prefatory note.

"Esta monografía fué presentada en la Universidad nacional como tésis para el grado de doctor en derecho público."--Prefatory note.

Proefschrift--Wageningen. / Includes bibliographical references (p. 32-346).

Thesis (M.A.)--University of Wisconsin--Madison, 1967. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Typescript. Tables. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (Master's) - Universitas Gadjah Mada, 1991. / Abstract in English. Includes bibliographical references (leaves 143-146).

Internodes 5-8, 10, 13 and 15 of Saccharum sp. var. NCo376 were examined for evidence of symplasmic phloem unloading of sucrose from the phloem, via the bundle sheath to the storage parenchyma. The vascular bundle possesses wellisolated phloem comprised of large diameter sieve elements and small diameter companion cells. A layer of phloem parenchyma surrounds the phloem, except where the phloem abuts the crushed protophloem. Outside this is a sclerenchymatous sheath, directly endarch to a parenchymatous bundle sheath, which is surrounded by storage parenchyma. The bundle sheath is interrupted at the centrifugal pole of the vascular bundle by a phloem fibre cap. Scanning Electron Microscopy revealed plasmodesmal fields throughout the bundle sheath and pith tissue. Transmission Electron Microscopy studies provided evidence of plasmodesmal occlusion, but not in all tissues. Aniline blue reactions under UV light indicate the presence of occluded plasmodesmal fields at the phloem parenchyma / sclerenchymatous sheath interface, and in localised regions of cells which are smaller than the surrounding storage parenchyma cells. This suggests a symplasmic transport pathway at these locations, and, based on these positive aniline blue reactions, with regulation via callose-mediated transplasmodesmal transport. Osmotic stress experiments, which included the addition of Ca2+, did not reveal further callose occlusion in the parenchyma, suggesting that the plasmodesmata in these regions may be closed via a noncallosic mechanism. Dye-coupling studies, using Lucifer Yellow (LYCH), which was iontophoretically injected following turgor-pressure equalisation, showed only rare, limited symplastic transport, usually only between the injected cell and one adjacent cell. Most injections did not result in transport of LYCH, suggesting either a lack of plasmodesmal connectivity, occlusion, or gating of any plasmodesmata present. This limited symplasmic transport, combined with the presence of occluded plasmodesmata at the phloem parenchyma / sclerenchymatous sheath interface suggests the presence of a two-domain phloem-unloading pathway. While symplastic transport may occur from the phloem to the sclerenchymatous sheath, further sucrose transport to the storage parenchyma appears to proceed apoplasmically from the sclerenchymatous sheath / bundle sheath interface, and into storage parenchyma cells across the cell wall and cell membrane via specialised sucrose transporters.

Orientador: Joao Antonio da Costa Andrade / Coorientador: Pedro César dos Santos / Banca: Antonio César Bolonhezi / Banca: Hermann Paulo Hoffmann / Resumo: A interação genótipos com ambientes (GxA) representa uma das principais dificuldades para a seleção nos programas de melhoramento genético de qualquer espécie, seja na fase de seleção ou na recomendação de cultivares, onde os pesquisadores buscam alternativas para minimizar seus efeitos. Entre as alternativas está a escolha de cultivares com ampla adaptação e boa estabilidade. Os resultados de vários ambientes podem ser sintetizados por estudos de adaptabilidade e estabilidade fenotípica, permitindo conhecer melhor a capacidade produtiva e adaptação frente às variações ambientais de novos genótipos. O objetivo deste trabalho foi verificar a adaptabilidade e estabilidade de clones de cana-de- açúcar, além da estabilidade dos ambientes, quanto à produtividade em toneladas de colmos por ha (TCH) e toneladas de pol por ha (TPH) no Estado de São Paulo. Foram avaliados 10 clones e duas testemunhas comerciais de maturação média- tardia em primeira soca em 10 experimentos colhidos em agosto de 2008, utilizando o método de regressão bissegmentada e o método AMMI (Additive Main Effects and Multiplicative Interaction Analysis). A interação GxA para o caráter Pol%cana (PC) não foi significativa e TCH e TPH foram altamente correlacionados (r=0,97), apresentando resultados similares para todas as análises realizadas. Por isso, apenas os resultados de TCH foram apresentados e discutidos. Os resultados dos dois métodos foram comparados e evidenciaram que os clones RB975201, RB975157, RB975932, RB975242 e RB975162 são os mais promissores devido à produtividade acima das testemunhas, a alta estabilidade verificada em um ou nos dois métodos e adaptabilidade ampla ou específica. Os ambientes Tarumã, Guariba e Piracicaba, foram os mais estáveis, podendo servir de referência para recomendações em outras áreas / Abstract: The genotype x environment interaction (GE) is one of the main difficulties for selection in breeding programs of any species, either at the stage of selection or recommendation of cultivars, where researchers seek alternatives to minimize their effects. Between the alternatives is the choice of cultivars with wide adaptability and good stability. The results of various environments can be synthesized by studies of phenotypic adaptability and stability allowing better understand the production capacity and adaptation to environmental variations ahead of new genotypes. The objective of this study was to verify the adaptability and stability of sugar cane clones, in addition the stability of environments, for productivity in tons of cane per hectare (TCH) and tons pol per hectare (TPH) in São Paulo State, Brazil. Were evaluated 10 clones and two commercial checks of medium-late maturation in first-ratoon of experiments harvested in August 2008, utilizing bissegmented regression method and analysis AMMI (Additive Main Effects and Multiplicative Interaction Analysis). The interaction for the character Pol%cane (PC) was not significant and TCH and TPH were highly correlated (r = 0.97), with similar results for all analyzes. Therefore, only the results of TCH were presented and discussed. The results of two methods were compared and evidenced that clones RB975201, RB975157, RB975932, RB975242 and RB975162 are the most promising due to yield above checks, high stability observed in one or both methods and broad or specific adaptability. The environments Tarumã, Guariba and Piracicaba, are the most stable and may use as reference for recommendations in other areas / Mestre

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