The diploma thesis captures the contemporary security situation of Slovakia, identifies security problems by which it feels threatened, then goes on to point out whom and what Slovakia wants to protect and which strategy it plans to use in order to do that. At the same time it identifies the main insecurities of EU as a whole and those of Russia. These two powers are seen to play a major role in formation of relationships of amity and enmity, of cooperation and hostility in European regional security supercomplex of which, Slovakia is inseparable part. To describe and conduct analysis, author applies theories and analytical tools formulated by Copenhagen School. Most used were analytical frameworks and concepts of securitization, regional security complexes and sectoral approach to security.

ROS are frequently associated with deleterious effects caused by oxidative stress. Despite the harmful effects of non-specific oxidation, ROS also function as signal transduction molecules that regulate various biological processes, including stem cell proliferation and differentiation. Here we show that mitochondrial ROS level determines cell fate during differentiation of the pluripotent stem cell line P19. As stem cells in general, P19 cells are characterized by a low respiration activity, accompanied by a low level of ROS formation. Nevertheless, we found that P19 cells contain fully assembled mitochondrial electron transport chain supercomplexes (respirasomes), suggesting that low respiration activity may serve as a protective mechanism against ROS. Upon elevated mitochondrial ROS formation, the proliferative potential of P19 cells is decreased due to longer S phase of the cell cycle. Our data show that besides being harmful, mitochondrial ROS production regulates the differentiation potential of P19 cells: elevated mitochondrial ROS level favours trophoblast differentiation, whereas preventing neuron differentiation. Therefore, our results suggest that mitochondrial ROS level serves as an important factor that directs differentiation towards certain cell types while preventing others.