The issue of the relationship between water discharge and the suspended sediment loads is a globally highly addressed topic. Knowing the suspended sediment loads in the streams avoids problems with over-filling of water cannons and thus prevents insufficient capacity of water reservoirs. This thesis is partly a follow-up to the bachelor thesis, which extends and introduces new procedures. Neural networks, more specifically multilayer perceptron neural networks, are used to analyse the relationship between water discharge and suspended sediment loads. The results of the networks are then processed in Excel into graphs and evaluated using the coefficient of determination, Nash-Sutcliffe coefficient and RMSE coefficient. The practical application is solved on two profiles - the profile Podhradí nad Dyjí and the profile Židlochovice. Each profile is examined in a different period.