This research investigates the ability of rat tapeworm (Hymenolepis diminuta) to accumulate zinc in tissue and the influence of its intake and excretion by its host (laboratory rat). The host was fed by food with zinc in two forms: 1) a mixture of standard food ST1 with hyperaccumulator plant Arabidopsis halleri, 2) ST1 mixture with zinc lactate, which is ordinarily used as a feed supplement for increasing zinc content and it is often included in human diet supplements. Rat control group fed by ST1 only was included in the experiment for verification of the difference. Rats were divided to six groups (OO, OT, RT, RO, MO a MT). Three rat groups were infected by rat tapeworm (OT, RT, MT) and three rat groups were not infected (OO, RO, MO). The control groups OO and OT were fed by ST1 only. RO and RT groups were fed by ST1 with admixture of Arabidopsis halleri and groups MO and MT were fed by ST1 with zinc lactate. Urine and excrements of the rats were collected twice a week during the experiment and their amounts were measured every day. The rats were weighted every week. At the end of the balance phase of the experiment rats were euthanized and seven selected tissues were removed (liver, kidney, spleen, small intestine, testis, muscle and bone). Rat tapeworm was removed from the infected rats. Blood was drawn from the rats. The results show that rat groups infected by the rat tapeworm had lower concentration of zinc in almost all analyzed tissues except for spleen, where the concentration of zinc was the same as in groups without the rat tapeworm. Based on the results the rat tapeworm also has an influence on the excretion of feces and urine.

No description available.

Heavy metals in living organisms occur in trace amounts. While zinc is an essential element wich is indispensable for many physiological processes, cadmium in body acts as atissue toxin, that can cause oxidative stress and contribute to cancer development. Recent studies suggest that some species of tapeworms (Cestoda) and Acantocephala are able to absorb in their tissue higher quantity of heavy metals in comparsion with their hosts. Moreover it was observed that In connection with the increased accumulation of heavy metals in the tissue of tapeworm was decreased amounts of these metals in the organs of the host. The aim of this thesis was to investigate whether the rat tapevorm (Hymenolepis diminuta) causes a decrease in the concentration of Cd and Zn in the organs of rats. A total of 24 Wistar rats (Rattus norvegicus) were divided into 4 groups (n=6). Groups PT and 0P take increased doses of kadmium (3,0 mg/week) and zinc (235,7 mg/week) for six weeks. This increased intake was achieved by adding Arabidopis halleri to feed. After six weeks rats were killed and concentrations in their tissues (bone, muscle, testes, spleen, intestine, liver kidney) were analyzed. Subsequent statistical tests confirmed lower levels of Cd and Zn in some organs of rats infected with tapeworm compared to rats without infection. For example in kidney, liver ant intestine of infected rats was detected 2,9; 2,5 and 1,8 times lower concentrations of cadmium and zinc in comparsion with uninfected rats. Tapeworm absorbed 1695 time higher amount of kadmium compared to the host muscle. The zinc content was lower in all organs (except spleen and testes) of infected rats in comparsion to uninfected. In kidney, liver and intestine of infected rats was 1,2; 1,25 and 1,4 times lower average concentration of zinc in comparsion with uninfected rats. The tape worm absorb higher concentration of zinc compared to all organs of the host.

In 2012 the tapeworm Echinococcus multilocularis in naturally infected red fox were investigated in selected regions of Karlovy Vary. From January to December 2013, in areas with the highest prevalence, delivery baits containing the anthelmintic (80 g of fish flesh + 50 mg Praziquantel- Drontal plus flavour) in an amount of 50 baits per km2. In 2014 the inspection was carried out investigations and subsequent comparison prevalence of tapeworm in the monitored areas between 2012 and 2014, ie. before and after the delivery of anthelmintic baits. The observed prevalence of Echinococcus multilocularis in foxes before delivery baits was 80% (12/15), after delivery baits was 10.5% (2/19). Furthermore, an analysis of infected intestinal tissue foxes and foxes uninfected with Echinococcus multilocularis for determining the concentrations of selected elements (Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn). Foxes with this tapeworm exhibited in the intestinal mucosa higher levels of the following elements: chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), zinc (Zn). Conversely concentration primarily toxic elements (Cd and Pb) in the intestine infected foxes with Echinococcus multilocularis was lower than in the intestine foxes without this parasite.

Redeskription of three bothriocephalid tapeworms Tetracampos ciliotheca, Polyonchobothrium polypteri and Senga gordoni from African freshwater fish.

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Dominik Jandura Supervisor: PharmDr. Ivan Vokřál, Ph.D. Title of diploma thesis: In vitro cultivation of tapeworm Hymenolepis diminuta - 2 Aim of this diploma thesis was to obtain cycticercoids of the rat tapeworm (Hymenolepis diminuta), excyst them and find out the conditions for the maximal in vitro incubation period. As the intermediate host mealworm beetle (Tenebrio molitor) infected by the rat feces containing tapeworm eggs was used. Excystment was done using L-cystein and sodium tauroglycocholate. Excysted larvae were cultured in vitro (37 řC, 5 % CO2) in RPMI 1640 medium enriched with other substances chosen according previously published methods. Mainly sheep, mouse or rat liver extracts eventually in combination with yeast extract and sheep bile were used. The effect of tested substances on the cultivation was evaluated by measuring of the tapeworm's growth. The best effect on the grow of the tapeworms was observed using medium containing serum, yeast extract and sheep liver extract where tapeworms achieved length of 1561 µm after 16 days of incubation. The further growth was limited by appearance of pathologic formations.

The aim of this thesis was to survey the problem of endoparasitic nematode, cestode, trematode and coccidia infections in sheep and their connection with the farming conditions and zoo-hygienic care. We have found out that 7 species of coccidia, 14 species of gastrointestinal nematodes, three species of lung nematodes, two species of cestodes and four species of trematodes can be found in local conditions. Further we discussed possible ways of suppressing the infections in the breeding environment. The practical coprological examination took place in autumn 2018. We've examined fecal samples from 10 different sheep farms in the Tišnov region, 100 samples in total. We've discovered four species of coccidia (Eimeria bakuensis, E. crandalis, E. parva, E. ovinoidalis), eight species of gastrointestinal nematodes (Bunostomum trigonocephalum, Cooperia curticei, Haemonchus contortus, Chabertia ovina, Nematodirus spp., Oesophagostomum venulosum, Trichuris ovis, Trichostrongylus spp.), two species of lung nematodes (Muellerius ca¬pillaris, Protostrongylus rufescens) and one cestode species (Moniezia expansa). Prevalence and intensity of the infection varied greatly among the farms. We concluded that administering feed supplements during lambing had the most noticeable positive effect on the infection course.

Diphyllobothriidean tapeworms are well-known parasites of mammals including man, but species parasiting in reptiles are much less known. These tapeworms belong to three genera (Bothridium, Duthiersia, Scyphocephalus) of the family Solenophoridae and are characterized by their unique scolex morphology. They occur in the intestine of varanid lizards and snakes. All three genera are known from Asia, two from Africa (Bothridium and Duthiersia) and one from Australia and South America (Bothridium). Individual genera are well characterised, but species composition of these genera is not well understood. This study surveyed available literary data on the genera Duthiersia and Scyphocephalus and provides new information based on new collected material from Africa and Southeast Asia and material deposited in helminthological collections.

The aim of the study was to assess the effects of orally applied praziquantel on the treatment efficacy against most abundant tapeworms (Atractolytocestus huronensis Anthony, 1958 and Khawia sinensis Hsü, 1935) of common carp (Cyprinus carpio Linnaeus). Naturally infected fish (one-year-old; K1+) were divided into 3 trial groups containing 17 specimens and administered with praziquantel mixed in heat-treated amyloid vehicle (in doses of 50 mg.kg-1 body weight). In 3 groups of control, the fish were administered amyloid vehicle only. Fish were examined by using of gut dissection 2, 4 and 6 days after administration. At 48 hours post-treatment, significantly lower prevalence of both parasites was found. The invasion of A.huronensis was fully eliminated in all fish after 96 hours whereas some specimens of K.sinensis still persisted in the gut of few fish. The full elimination of both parasites was observed 6 days after administration. Our results showed that elimination of K. sinensis takes longer than elimination of A.huronensis. The dose of 50 mg kg-1 of orally applied praziquantel is effectual to control A.huronensis and K sinensis infections in common carp successfully. The effect of oral applied praziquantel on the hematology and biochemical parameters blood of common carp (Cyprinus carpio L.), infected by the tapeworms Atractolytocestus huronensis and Khawia sinensis was the object of analyse. It was the partial goal of the work. The infected fishes were divided to the six groups. The praziquantel was applied stirred in amyloid vehicle in dose 30 mg/kg of quick materiality for two groups and for two to other groups in dose 50mg/kg of quick materiality. Only clean amyloid vehiculum was applied to two control groups. Blood taking and resulting patoanatomic investigation was effected 24 and 96 hours after application of praziquantel. Results of hematology investigation of blood obtained 24 hours after the application point on significant fall (p< 0,01) of the number of erythrocytes, hemoglobin and hematocrit in both groups E1 and E3, which achieved the original surface after 96 hours. The significant upsurge (p<0,05) of the surface serum hepatic enzyme ALT was recorded after 96 hours.

Mesocestoides corti and Taenia crassiceps are tapeworms, larvae of which are characterized by their ability to reproduce asexually. In this work, the effect of infection by M. corti and T. crassiceps in BALB/c, C57BL/6J and ICR mice on the growth and metastasis of B16F10 melanoma tumors was investigated. Although an increase in metastatic activities was observed after intravenous administration of melanoma cells to M. corti-infected mice, both tapeworms showed a strong suppressive effect on the size and number of tumors and metastases formed when the cells were administered intraperitoneally. This, in some cases, led to a complete elimination of tumor cells. In vitro cultivation of B16F10 cells in the presence of larval excretory-secretory products led to a decrease in their viability but an increase in their migration ability. Flow cytometry proved that M. corti infection has an effect on the increased number and proportion of macrophage populations in the peritoneum of ICR mice. Our work confirmed the anti-tumor effect of T. crassiceps infection in mice and introduced M. corti as a new helminth species capable of influencing cancer. Key words: helminths, cestodes, cancers, Mesocestoides corti, Taenia crassiceps