Thesis (Ed.M.)--Boston University

Thesis (Ed.M.)--Boston University

Thesis (M.S.)--Boston University

Over the past decade, the illicit drug market has experienced an explosion of designer drugs being produced by clandestine laboratories that include modifications of illicit drugs that dominated drug markets for long periods of time (e.g. cathinones and MDMA). These designer drugs, which are commonly known as “legal highs”, are popular due to that fact that they are legally obtainable and not currently controlled. Examples of these include the phenylalkylamine analogues 5-iodo-2-aminoindane (5-IAI) and 5, 6-methylenedixoy-2-amonindane (MDAI), which have similar biological effects to MDMA. In spite of the unknown risk factors associated with these substances, it is believed that “legal highs” continue to have high levels of interest among recreational users. As such, the potential for abuse is high, and 5-IAI and MDAI are under consideration in numerous jurisdictions for regulation. Many of these novel compounds have never been analyzed previously within a forensic setting. The chemical and physical properties of 5-IAI and MDAI are not fully understood. As a result, the analytical analysis of “legal highs” can be challenging. Color test kits provide a quick screening method for law enforcement officials looking to presumptively identify a substance in the field. The difficulty with this form of analysis is that most of the active ingredients present in “legal highs” are not detected by standard presumptive tests, or the results when the tests are used are ambiguous. Gas chromatography-mass spectrometry (GC-MS) is one of the most utilized analytical instruments in forensic laboratories for the identification of drugs of abuse. However, due to the rapid development and commercialization of “legal highs,” the limited availability of certified reference standards and mass spectral data make the confirmatory analysis of “legal highs” challenging. The primary aims of this research were two-fold. The first was to evaluate selected commercially available Narcotics Analysis Reagent Kits (NARK® II) and color reagent formulations recommended by the National Institute of Justice (Color Tests Reagents/ Kits for Preliminary Identification of Drugs of Abuse) to determine if the phenylalkylamine analogues 5-IAI and MDAI generate a color development. If a color was generated using a particular reagent, further testing was conducted to establish if the observed color would be detectable in the presence of various adulterants. The second aim of this research was to develop a rapid GC-MS method for the detection of 5-IAI and MDAI in contrived multi-component mixtures of selected adulterants. Standard color tests provided consistent results for 5-IAI and MDAI pure samples as well as mixtures with adulterants. 5-IAI produced a light brown color with both the Marquis and the methylenedioxypyrovalerone (MDPV) color reagent tests. The Mandelin reagent from the NARK® II test kit produced a greenish brown color and a light green color with the In-House preparations of the same reagent when tested with MDAI. Confirmatory analysis was performed using GC-MS with a temperature gradient. The analysis was performed on a non- polar (5% phenyl) methylpolysiloxane column with a total run time of 10 minutes. 5-IAI and MDAI were chromatographically separated and distinguishable from various adulterants based on retention time and mass to charge ratio.

Thesis (Ph.D.)--Boston University / PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. / Normal hearing is typically defined by threshold audibility, or the loudness of sounds that an individual is able to hear. This convention exists despite the fact that everyday communication relies on extracting and interpreting features of supra-threshold sound. Many normal-hearing listeners struggle to perform certain supra-threshold auditory tasks, and debate persists as to whether such difficulties originate from deficits in cognitive processing or in peripheral, sensory encoding. In this work, we show that there are large individual differences among normal-hearing adults (18 to 55 years), who were asked to report a stream of digits located directly ahead in a simulated rectangular room. Simultaneous, competing masker digit streams were simulated at locations 15° left and right of center, and the level of reverberation was adjusted to vary task difficulty. Performance was best in the anechoic condition and worst in the high-reverberation condition, but listeners nearly always reported a digit from one of the three competing streams, showing that reverberation did not render the digits unintelligible. Most importantly, inter-subject differences were extremely large. These differences were not significantly correlated with age, memory span, or hearing status. They were, however, correlated with behavioral differences in the ability to detect small frequency modulations in pure tones and with the strength of the frequency following response (FFR), a physiological measure of spectro-temporal detail encoding in supra-threshold sounds early in the auditory pathway. The decomposition of the FFR into envelope and carrier components shows that envelope frequency following is the preferred cue for completing the spatial attention task, but it degrades with early aging. As a result, older listeners depend more on carrier phase locking cues than younger people. These results suggest that differences in peripheral encoding help explain individual differences in the ability to communicate in challenging settings, but that aging also has a separate, dissociable effect on early encoding in the auditory system. Tests like these may help tease apart contributions of peripheral and central deficits to communication impairments, ultimately leading to new approaches for helping listeners cope with complex listening environments. / 2031-01-02

Digitized by Kansas Correctional Industries

Digitized by Kansas Correctional Industries

Thesis (Ed.M.)--Boston University / PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you. / 2031-01-01

Digitized by Kansas Correctional Industries

L’évaluation du comportement animal est devenue un outil fondamental dans les champs de recherche des neurosciences translationnelles. Elle est en effet utile pour l’étude des mécanismes physiologiques entraînant les maladies neurodégénératives mais aussi pour la compréhension des modifications fonctionnelles induites par des manipulations génétiques ou traitement chimique et pour tester l’efficacité de composés ou molécules. Chez l’Homme, on utilise des batteries de tests cognitifs et moteurs pour qualifier et quantifier les atteintes des maladies neurodégénératives telles que les maladies d’Alzheimer et de Parkinson. Le travail présenté ici s’inscrit dans l’optique de promouvoir Microcebus murinus comme modèle pour l’étude des maladies neurodégénératives liées à l’âge. Le microcèbe est un petit primate lémurien, proche phylogénétiquement de l’Homme, présentant des particularités prometteuses susceptibles d’accélérer la découverte de nouveaux traitements curatifs et prophylactiques pour les pathologies liées à l’âge. La validation du modèle nécessite l’utilisation de tests comportementaux discriminant l’atteinte neurodégénérative. Nous avons mis au point un ensemble de tests comportementaux évaluant l’apprentissage, la mémoire et les fonctions motrices. Des animaux sains ont été évalués et ont permis de définir des protocoles spécifiques au microcèbe. Ces protocoles ont ensuite été appliqués à des animaux ayant subi un traitement pour induire des pathologies de type Parkinson ou Alzheimer afin de détecter l’apparition d’éventuels déficits. / Animal behavior has become a fundamental tool in translational neuroscience area and is useful for studying physiological mechanisms underlying neurological diseases and also for understanding the functional modifications induced by genetic manipulation or chemical treatment. The experiment of new treatments requires animal models miming the human pathology. In Humans batteries of cognitive and motor tests are used to qualify and quantify the impairment due to neurodegenerative disease as Alzheimer's disease and Parkinson’s disease. The aim of this work is to promote Microcebus murinus as a model of age related neurodegenerative pathologies. The grey mouse lemur, a small prosimian primate, phylogenetically close to Human, presents specific characteristics susceptible to provide important information on the validity and efficacy of new. The validation of a model requires the use of behavioral tests to discriminate neurodegenerative impairment. A set of behavioral tests were worked out to evaluate learning memory and motor functions. Healthy animals were evaluated and allowed to define protocols species specific. These protocols were then applied on animals treated to induce pathology such as Parkinson or Alzheimer in order to detect cognitive or motor impairments.