Thesis (M.S.)--University of Wisconsin--Madison, 1969. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1968. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Toxaphene is the most abundant persistent organic pollutant in the Arctic and in the Great Lakes. Toxaphene technical mixture (Tox) applied as a pesticide consists of over 800 congeners. Through processes of environmental degradation, selected metabolism, and bioaccumulation, 2 congeners are prominent in humans; 2-exo,3-endo,5-exo,6-endo,8,8,10,10-octachlorobornane (T2) and 2-exo,3-endo,5-exo,6-endo,8,8,9,10,10-nonachlorocamphene (T12). The MCF7-E3 human breast cancer cell model was used to screen for the estrogenic activities of Tox, T2, and T12. A concentration of 10muM was required by all 3 compounds to elicit an estrogenic response (a proliferative effect (PE) upon the cells). The congeners however, showed slightly different PEs from Tox. Both T2 and T12 had a lower PE than Tox, and T2 had a higher PE than T12. Results from binary combination studies showed that effects of Tox, T2, and T12 were additive. Tox, T2, and T12 had no significant effects on estrogen receptor and progesterone receptor levels. It was shown that the environmental prevalent congeners had lower estrogenic activities than Tox and there is no synergistic effect.

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Effects of Toxaphene®, a polychlorinated terpene insecticide, were studied by a multidisciplinary approach on two cockroach species, Leucophaea maderae (F.) and Periplaneta americana (L.). Toxaphene was found to be slightly toxic to L. maderae and moderately toxic to P. americana. Uptake of ³⁶C1-toxaphene in tissues of L. maderae was studied from 2 to 120 hours. Greater accumulation of ³⁶C1-toxaphene on a ug/gm basis was found associated with the abdominal ventral nerve cord (VNC) of L. maderae than with haemolymph, fat body, and alimentary canal. ³⁶C1-toxaphene uptake was noted in L. maderae VNC in vitro at levels which were independent of incubation dose concentration. Substantial amounts of ³⁶C1-toxaphene remained associated with VNC after rinsing. Radiolabeled material found in haemolymph of asymptomatic L. maderae appeared to be qualitatively different from toxaphene standards and also that found in symptomatic insect haemolymph based on gas chromatography. Concentration levels responsible for in vivo toxicity appeared to correlate with electrophysiological symptomology in VNC of P. americana. Intense forms of activity were observed in isolated nerve preparations and in VNC removed rom symptomatic insects treated with 1/10⁵ and 1/10¹ M toxaphene. Latent periods between introductioon of toxaphene and onset of intense activity appeared to vary as a function of concentration.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

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