Trans-frontier conservation areas (TFCAs), large cross-border areas dedicated to biodiversity conservation, multi-national co-operation and development are expanding in southern Africa, fast becoming the dominant conservation solution in the region. TFCAs adopt a celebratory discourse of ecological, community, economic and political gains, while the reality is often far more complicated. This thesis situates the expansion of TFCAs within a critical political ecology approach, and argues that they represent a neoliberal solution to a complex series of development, environment and political challenges. Drawing on five and a half months of fieldwork to Mozambique along with policy and discourse analysis it examines the first marine reserve to be linked to a TFCA in Africa, the Ponta do Ouro Partial Marine Reserve (PPMR) in southern Mozambique. It makes three arguments: First, it argues that Mozambique’s embrace of TFCAs represents the neoliberalisation of conservation through novel tourism-based products, techniques of governance, creation of subject positions based on entrepreneurialism, and new arrangements of space. At the same time, the adoption of TFCAs also stems from Mozambique’s post-war politics, especially the ways in which elite state actors have sought to reconstruct and reorder the country through engagement with donors. Second, the thesis uses a combined governmentality and assemblage framework to explore how neoliberal conservation is made to cohere as a truth discourse, how it materially co-produces human and non-human life in the marine reserve, and how it is fragile, partial and contested. Third, it critiques the increasingly close relationship between the extractive and conservation sector at a policy, state and donor level, exploring how and why marine conservation is increasingly intertwined with Mozambique’s resources boom through its green economy discourse. Through these three points of engagement, the thesis contributes to debates around the intensifying relationship between extraction and conservation, Mozambique’s post-war development, and processes of neoliberalisation of nature.

Submitted by Alexia Roxana Zúñiga Pérez (alexia.zupa@gmail.com) on 2017-12-01T21:46:28Z No. of bitstreams: 1 ALEXIA_ZÚÑIGA_Dissertação.pdf: 13223947 bytes, checksum: 5c3355433648347b20822de32af829cf (MD5) / Approved for entry into archive by Lêda Costa (lmrcosta@ufba.br) on 2018-04-24T19:08:20Z (GMT) No. of bitstreams: 1 ALEXIA_ZÚÑIGA_Dissertação.pdf: 13223947 bytes, checksum: 5c3355433648347b20822de32af829cf (MD5) / Made available in DSpace on 2018-04-24T19:08:21Z (GMT). No. of bitstreams: 1 ALEXIA_ZÚÑIGA_Dissertação.pdf: 13223947 bytes, checksum: 5c3355433648347b20822de32af829cf (MD5) / CAPES / O estudo aqui apresentado tem sua origem entre 2013 e 2014, durante um trabalho desenvolvido pela autora na Secretaria de Saúde do México – órgão do Governo Federal – onde, trabalhando na realização de uma reportagem sobre aposentadoria, teve interesse em saber sobre as pessoas idosas trans, pelo simples e duro fato delas não constarem nos números dessa Secretaria. A tentativa foi procurar por essas pessoas para torná-las visíveis e tornar visíveis suas histórias. Para isso, optou-se pela fotografia como elemento poético operativo, por meio da qual se propõe construir uma ponte entre o processo de criação e as experiências de vida das pessoas escolhidas para a pesquisa com a vida da pesquisadora. Assim, as reflexões aqui realizadas atravessam os temas da memória, das decisões de vida, do olhar, do reconhecimento de si no outro e vice-versa, passando pelo uso de um discurso que mistura realidade e ficção na trama fotográfica a partir do resgate de imagens das lembranças oferecidas pelas fotografias antigas e aquelas contidas nos álbuns familiares. Trata-se, portanto, de um relato poético sobre a transformação, no qual, por meio das imagens produzidas, se relaciona com a teoria, com o processo criativo do trabalho visual e com a história de vida das participantes num diálogo honesto e constante. Palavras chaves: Fotografia, transformação, idosas trans, ficção, invisibilidade.

No description available.

According to the material feminist corpus, namely Stacy Alaimo’s concept of trans-corporeality, material flows and interconnectivity between humans and their environment insists that the human body has never been atomistic, but rather a porous figure that continually interacts/intra-acts with its environment. The recent biotechnological boom allowing for the production of human-animal hybrids (chimeras) provides the kind of visualization of these interconnectivities that can help instigate a reconception of the human—as not human at all, but rather posthuman. This study looks at the presence of these human-animal hybrids in popular art media, specifically: the horror film, Splice (Dir. Natali 2009); the YA novel, Inhuman (Falls 2013); and the comic, Sweet Tooth (Lemire 2009-2013). This thesis argues that the human-animal hybrid figure exhibits utopian horror, or the use of horror to produce new, better, ways of conceptualizing human-animal relationships, ones that acknowledge our already posthuman plurality of self.

Made available in DSpace on 2015-11-04T13:39:10Z (GMT). No. of bitstreams: 2 tainah_paula_ioc_dout_2013.pdf: 4720016 bytes, checksum: abb2c1119571eec6ea090739b5bbc8a7 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2015-10-29 / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / A regulação gênica em tripanossomatídeos é policistrônica e ocorre de maneira pós-transcricional. Nas extremidades 5' e 3' dos RNA mensageiros existem segmentos que podem conter elementos regulatórios chamados UTR (Untranslated Regions), os quais são regiões transcritas, porém não-traduzidas. Para verificar se UTR de tamanhos diferentes possuem impacto na transcrição e, por conseguinte, na tradução de proteínas, selecionou-se genes da família de trans-sialidases, devido à importância destas no processo de invasão celular. A metodologia envolveu a extração de RNA total de T. cruzi CL-Brener, seguido da síntese de cDNA, PCR, clonagem dos produtos amplificados e seqüenciamento por Sanger e pelo uso do 454 Junior (Next Generation Sequencing \2013 NSG). Como as trans-sialidases correspondem a uma família gênica de cópias múltiplas, usou-se a estratégia de sequenciamento de alto-desempenho na tentativa de cobrir o maior número possível de genes desta família. Para isso foram obtidos cDNAs de trans-sialidases de CL-Brener nas formas epimastigota e tripomastigota com o iniciador 5\2019UTRTCNA, os quais apresentaram UTR com tamanhos variados entre 65 \2013 187 pb, além da obtenção de cDNAs para esta família de proteínas, em epimastigotas CL-Brener, com o iniciador 5\2019TcTS, apresentando UTR variando de 171 a 221 pb, com similaridade de sequências entre elas. Com o intuito de avaliar a correspondência entre a transcrição dos RNAs de trans-sialidases e a sua tradução, houve a necessidade de identificação das proteínas. Desenvolvemos uma metodologia de lise celular e produção de extrato proteico (denominado TcS12) a partir de células de T. cruzi no estágio epimastigota. As cepas selecionadas para esse estudo foram CL-Brener (TcVI), Dm28c (TcI), Y (TcII) e 4167 (TcIV) O processo de lise celular foi otimizado para 107 parasitos/mL ressuspensos em 200 \03BCL de tampão de lise hipotônica (por 30 minutos a 40C), associado ao sonicador de banho (por 30 minutos a 40C). A eficiência da metodologia foi validada pela citometria de fluxo mostrando que aproximadamente 72% das células foram marcadas com iodeto de propídeo (PI). A qualidade dos extratos proteicos foi analisada por LCMS/MS usando-se a estratégia MSE label free para quantificação relativa de proteínas. Foram identificadas 1153 proteínas totais, cuja expressão proteica das cepas 4167, Dm28c e Y, quando comparada à CL-Brener (cepa referência), apresentou 32, 51 e 73 proteínas up-expressed, enquanto que 80, 92 e 60 proteínas mostraram-se down\2013expressed, respectivamente. Entre as trans-sialidases identificadas, a única cópia encontrada no extrato TcS12 (número de acesso no UniProt - Q4DGV8) apresentou seu RNAm correspondente no banco de dados de cDNA de CL-Brener, com UTR de 214 pb, estando, portanto, na faixa de tamanhos das outras UTR de trans-sialidases obtidas neste estudo. Esse resultado sugere que outros fatores, não necessariamente o tamanho per se das UTR, podem influenciar no fenômeno de tradução nesses tripanossomatídeos. Em relação aos extratos proteicos de epimastigotas gerados a partir das outras cepas, a quantidade de trans-sialidases identificadas foram de 1 (cepa 4167), 2 (cepa Dm28c) e 117 (cepa Y) cópias, indicando assim a tradução satisfatória de pelo menos uma das muitas cópias desta família gênica / Gene regulation in trypanosomatids is polycistronic and occurs in a post - transcriptional way. There are also regulatory elements named UTRs (Untranslated Regions) that are transcribed regions, but not translated. To verify the impact of UTRs presenting different sizes in the transcription machinery and protein translation, genes f rom trans - sialidas e family were selected due to its importance in the cell invasion process. The methodological strategies involved the extraction of total RNA from T. cruzi CL - Brener strain , followed by cDNA synthesis, PCR, cloning and sequencing of amplified products by Sanger and by using the 454 Junior (Next Generation Sequencing – N G S ). Considering that trans - sialidase is a multi - copy gene family, this high - throughput sequencing strategy was employed in an attempt to cover the largest number of trans - sialidase genes. Trans - sialidase cDNAs from CL - Brener epimastigote and tripomastigote were obtained with 5`UTRTCNA primer show ing UT R sizes between 65 - 187 bp. The cDNA from this protein fam ily were also obtained with the 5’TcTS primer from CL - Brener epimastigote s , generating UTRs with 171 - 221 bp . Both 5’UTR presented sequence similarit ies between them. In order to evaluate the correspondence between trans - sialidase gene transcription and t ranslation, it was necessary to accomplish the identification of proteins. Therefore, we developed a methodology for cell disruption, which resulted in a protein extract ( referred as TcS12 ) from epimastigote T. cruzi cells. The strains selected for this st udy were CL - Brener (TcVI), Dm28c (TcI), Y (TcII) and 4167 (TcIV). The process for lysing the cells was optimized to 10 7 parasites/mL resuspended in 200 μL hypotonic lysis buffer (30 minutes at 4 o C) , followed by water bath sonication (30 minut e s a t 4 o C) . The process effic acy was confirmed by FACS , showing that near 72% of the cells were successfully stained with propidium iodide solution (PI). The quality of the protein extracts was analyzed by LCMS/MS using the strategy MS E label free for relative quant ification of proteins. A totality of 1153 proteins were identified and the comparison of the expression profiles between the strains 4167, Dm28c and Y , using the CL - Brener as reference, showed 32, 51 and 73 proteins up - expressed, and 80, 92 and 60 proteins were shown to be down - expressed, respectively. We observed that 117 trans - sialidase s were identified in Y strain, whilst in 4167, Dm28c and CL - Brener were found 1, 2 and 1 trans - sialidases, respectively. Moreover only one copy of trans - sialidase found in the TcS12 extract (UniProt accession number - Q4DGV8), also met its corresponding mRNA in the CL - Brener cDNA database, presenting an UTR of 214 bp, in the size range of the others trans - sialidase UTRs obtained herein. This result suggests th at other facto r s, but not exclusively the UTR sizes per se , could be related to the translation phenomenon in these trypanosomes

Programa de Pós-Graduação em Engenharia Ambiental. PROÁGUA, Pró-Reitoria de Pesquisa e Pós Graduação, Universidade Federal de Ouro Preto. / Submitted by Oliveira Flávia (flavia@sisbin.ufop.br) on 2014-12-11T16:26:50Z No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) DISSERTACAO_AvaliaçãoInfluênciaEmissões.pdf: 2479192 bytes, checksum: 1c08c947065974190ec3534f0bd40fcb (MD5) / Approved for entry into archive by Gracilene Carvalho (gracilene@sisbin.ufop.br) on 2014-12-14T12:46:47Z (GMT) No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) DISSERTACAO_AvaliaçãoInfluênciaEmissões.pdf: 2479192 bytes, checksum: 1c08c947065974190ec3534f0bd40fcb (MD5) / Made available in DSpace on 2014-12-14T12:46:47Z (GMT). No. of bitstreams: 2 license_rdf: 22190 bytes, checksum: 19e8a2b57ef43c09f4d7071d2153c97d (MD5) DISSERTACAO_AvaliaçãoInfluênciaEmissões.pdf: 2479192 bytes, checksum: 1c08c947065974190ec3534f0bd40fcb (MD5) Previous issue date: 2009 / O benzeno, substância comprovadamente carcinogênica, é um composto onipresente na natureza. Seus efeitos tóxicos crônicos envolvem principalmente alterações hematológicas, como leucemias, além de influenciar diversos tipos de danos ao DNA. Existem diferentes fontes antropogênicas de emissão de benzeno, além de fontes naturais, sendo as principais fumaça de cigarro, escapamentos de automóveis, processos de abastecimento em postos de combustíveis e emissões industriais, principalmente de indústrias siderúrgicas e petroquímicas. As emissões mais significativas de benzeno nas siderúrgicas ocorrem durante o processo de coqueificação e são oriundas principalmente nas coquerias, normalmente presentes nessas indústrias. Tanto estas emissões quanto as das petroquímicas, são previstas por leis ocupacionais que instituem limites de exposição aos trabalhadores desses setores. As emissões de benzeno em áreas ocupacionais são legalmente controladas em todo o mundo, mas, poucos países legislam sobre a exposição não ocupacional. O Brasil não apresenta regulamentação quanto às concentrações atmosféricas fora de ambientes ocupacionais. Devido a esses fatores, nesta pesquisa foi avaliada a ocorrência de diferenças estatísticas no teor de ácido trans, trans-mucônico (ATTM) urinário, indicador biológico de exposição ao benzeno, entre moradores de uma cidade que possui uma grande indústria siderúrgica na área urbana. O ATTM foi extraído da urina através de cartucho contendo trocador iônico forte (SAX) e foi determinado por cromatografia líquida de alta eficiência, utilizando coluna C18 e detecção por UV-vis com arranjo de diodos. O método foi otimizado e validado com relação à linearidade, faixa de trabalho, precisão e exatidão, que apresentou resultados entre 78% e 98%. O método apresentou os limites de detecção e de quantificação iguais a 1,0 e 3,5μg.L-1, respectivamente. Foram determinadas as concentrações de ATTM em 193 amostras de urina coletadas em quatro sítios em duas campanhas de amostragem durante períodos de chuvas e estiagem. A concentração média de ATTM urinário encontrada foi 59,2μg.g-1 de creatinina. Não foi encontrada diferença estatística significativa ao nível de 95% de confiança com relação ao teor de ATTM urinário entre as duas campanhas, mas, nesse nível de confiança constatou-se diferença entre os resultados de indivíduos residentes em locais sob influência direta das emissões das coquerias e os resultados daqueles residentes em locais sem essa influência. Também foi encontrada diferença estatisticamente significativa ao nível de 95% de confiança entre os voluntários fumantes e não fumantes. ______________________________________________________________________________________________ / ABSTRACT: Benzene, a confirmed human carcinogen, is a ubiquitous environmental substance. Its chronic toxic effects are mainly haematopoietic effects, like leukaemia, and DNA damages. Besides natural sources of benzene, there are many anthropogenics sources, that the principal are tobacco smoke, exhaust emissions and evaporation losses from motor vehicles, evaporation losses during the handling, distribution and storage of fuels and industrial discharge, mainly from petroleum industry and steel industry. The significant emissions of benzene in the steel industry occur during the coke production processes, in a coke oven plant, usually existing in this industry. Both petroleum industry and steel industry emissions are due to the occupational laws that establish exposure limits for its workers. Benzene’s emissions in occupational areas are lawfully supervised around the world, but few countries have determined non occupational laws. Brazil hasn’t benzene atmospheric emissions regulation for general places. Due to this facts, in this work was evaluated the occurrence of significant statistics difference in the trans,trans-muconic acid (TTMA) urinary contents, biomarker exposure from benzene, among inhabitants from a city that has a steel industry in the urban area. The TTMA was clean-up through ionexchange (SAX) cartridge and it was determined by HPLC using a C18 column and UVVis detection with arrays of diodes. The method was optimized and validated about linearity range, work range, precision and accuracies, that showed results between 78% and 98%. It showed too, detection and quantification limits of 1,0 e 3,5 μg.L-1, respectively. Altogether, the TTMA urinary concentration was determined in 193 samples collected in four different places in two different periods, rainy and dry seasons.The mean TTMA urinary concentration was 59,2μg.g-1 creatinine. No statistically significant difference at the 95% level confidence was found between TTMA urinary concentrations in both periods. A difference statistically significant at 95% confidence level was found between TTMA urinary concentrations of the one group of voluntary living in areas with direct influence from coke oven plants emission and another living in places without such influence. The difference between the TTMA urinary concentration of smokers and nonsmokers was statistically significant at 95% confidence level.

It has been estimated that 15 million individuals around the world experience the ill effects of neural disabilities every year. Neural disabilities can affect motor control, such as Locked in Syndrome or Amyotrophic Lateral Sclerosis, whereas other affect working memory, such as schizophrenia, Alzheimer's and Parkinson's. However, recent research has show that mental rehearsal of physical movement tasks may remain intact following higher centre damage, and as such represents a new opportunity to accessing the motor system and using it to control devices. Brain Computer Interfaces (BCI) captures the brain's electrical activity and translates it into real time electrical outputs, independent of the orthodox output pathways of peripheral nervous system and muscles. Utilising the brain's electrical activity BCI has the potential to significantly enhance the lives of many individuals suffering from neurological disorders. Unfortunately, the electrical activity associated with motor activity in these individuals can be lower than normal, with acute cortical infarcts decreasing the alpha wave oscillations for the affected pericentral sensorimotor areas. This has brought into doubt whether the intensity of brain signals in these individuals can be large enough to be used as a BCI system control signal for biofeedback training. This thesis aims to examine both if alternative EEG signal can be used and if externally applied neuromodulation can facilitate the process.

abstract: The goal is to provide accurate measurement of the channel between a ground source and a receiving satellite. The effects of the the ionosphere for ground to space propagation for radio waves in the 3-30 MHz HF band is an unstudied subject. The effects of the ionosphere on radio propagation is a long studied subject, the primary focus has been ground to ground by means of ionospheric reflection and space to ground corrections of ionospheric distortions of GPS. Because of the plasma properties of the ionosphere there is a strong dependence on the frequency of use. GPS L1 1575.42 MHz and L2 1227.60 MHz are much less effected than the 3-30 MHz HF band used for skywave propagation. The channel between the ground transmitter and the satellite receiver is characterized by 2 unique polarization modes with respective delays and Dopplers. Accurate estimates of delay and Doppler are done using polynomial fit functions. The application of polarimetric separation of the two propagating polarizations allows improved estimate quality of delay and Doppler of the respective mode. These methods yield good channel models and an effective channel estimation method well suited for the ground to space propagation. / Dissertation/Thesis / Masters Thesis Electrical Engineering 2017

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-09T23:53:03Z No. of bitstreams: 1 AndreLucasGuerreiroOliveira_DISSERT.pdf: 1295880 bytes, checksum: 7b4f7a62154f3d1593f3ae79a30c7ace (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-14T21:54:59Z (GMT) No. of bitstreams: 1 AndreLucasGuerreiroOliveira_DISSERT.pdf: 1295880 bytes, checksum: 7b4f7a62154f3d1593f3ae79a30c7ace (MD5) / Made available in DSpace on 2016-03-14T21:54:59Z (GMT). No. of bitstreams: 1 AndreLucasGuerreiroOliveira_DISSERT.pdf: 1295880 bytes, checksum: 7b4f7a62154f3d1593f3ae79a30c7ace (MD5) Previous issue date: 2015-02-27 / Esta pesquisa buscou analisar o discurso dos homens (trans) brasileiros em rela??o ? (des) patologiza??o das identidades (trans). Para tanto, buscou-se analisar a percep??o dos sentidos atribu?dos pelos homens (trans) brasileiros ?s suas experi?ncias inseridas no contexto em que sua viv?ncia ? considerada uma patologia psiqui?trica, pass?vel (ou n?o) de ser laudada por profissionais das ?reas m?dica e psicol?gica. De tal maneira que, a obten??o deste parecer torna-se obrigat?rio para a realiza??o de altera??es corporais, bem como aos encaminhamentos para as modifica??es da documenta??o civil. A metodologia adotada foi a an?lise de discurso e as t?cnicas de pesquisa foram entrevistas semiestruturadas e observa??o sistem?tica das reuni?es do grupo Cartografias Trans, grupo de atendimento psicoter?pico ? pessoas (trans) do Centro de pesquisa e atendimento ? travestis e transexuais ? CPATT em Curitiba, Paran?. O referencial te?rico da pesquisa apoiou-se principalmente, mas n?o apenas, nos estudos propostos pela teoria queer. Nesse sentido a pesquisa verificou a partir da inser??o no campo e da an?lise que os dados trouxeram, dentre outras quest?es, a exist?ncia de um discurso estrat?gico por parte dos homens (trans) brasileiros em rela??o ? despatologiza??o de suas identidades, manifestado principalmente na no??o de que ter a identidade (trans) patologizada n?o os torna doentes. / This research analyzed the discourse of Brazilian transmen in relation to (un) pathologizing of identity (trans). Therefore, it seeks to analyze the perception of the meanings attributed by Brazilian transmen to their experiences within the context in which their experience is considered a psychiatric disorder, which can be (or not) be diagnosed by doctors and psychologists. Whereby, obtaining such permissions becomes a necessity to perform bodily changes, as well as referrals for modifications of civil documentation. The methodology used was discourse analysis and research techniques were semi-structured interviews and systematic observation of the meetings of Cartografias Trans group, psychotherapeutic care group of transpeople to the Centro de pesquisa e atendimento ? travestis e transexuais - CPATT in Curitiba , Paran?. The theoretical framework of the research suppoted mainly, but not only, in the studies proposed by queer theory. In this sense the research found by entering the field and analyzing the data brought, among other things, the existence of a strategic discourse by Brazilian transmen toward depathologization of their identities, manifested mainly on the notion of to have the identity (trans) pathologized does not make them sick.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.