Made available in DSpace on 2014-06-12T16:31:30Z (GMT). No. of bitstreams: 2 arquivo6102_1.pdf: 1486883 bytes, checksum: 0b00e15632b2035cbae205b8ef4120e5 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005 / O objetivo deste trabalho foi obter microesferas de PLGA contendo trans-desidrocrotonina, caracterizá-las físico-quimicamente, avaliar seu perfil de liberação in vitro e atividade hipoglicêmica in vivo. As microesferas de PLGA foram obtidas pelo método de emulsão múltipla seguido da evaporação do solvente. A morfologia das microesferas foi avaliada por microscopia óptica e microscopia eletrônica de varredura. O doseamento químico da DCTN encapsulada nas microesferas de PLGA foi quantificado por espectrofotometria de ultra-violeta (UV). A cinética de liberação in vitro das microesferas foi realizada através da dissolução em tampão fosfato pH 7,4, simulando as condições fisiológicas do organismo. Em intervalos de tempo predeterminado as amostras foram coletadas , filtradas e armazenadas para posterior análise do conteúdo da DCTN. A avaliação in vivo foi realizada em camundongos albinos Swiss normais e diabéticos, induziu-se a diabetes com estreptozotocina; a DCTN (50 mg.Kg 1) foi administrada pela via oral durante 7 dias e a glicemia avaliada diariamente. Os resultados demonstraram que a eficiência da encapsulação foi de 85,46 ± 3,87 %. As partículas apresentaram forma esférica e tamanho médio de 3,11 ± 0,56 μm. A cinética in vitro mostrou um perfil de liberação gradual que atingiu aproximadamente 60 % em 3 dias. A DCTN encapsulada apresentou uma melhoria na biodisponibilidade, reduzindo a glicemia de animais normais, no entanto nenhum resultado estatisticamente significativo foi obtido nos animais acometidos pela diabetes nesta dose durante uma semana. Microesferas contendo DCTN possuem um uso potencial no controle de alguns estados hiperglicêmicos

Made available in DSpace on 2018-08-01T23:37:04Z (GMT). No. of bitstreams: 1 tese_10219_Dissertação Completa PPGE UFES Rodrigo Bravin.pdf: 2203051 bytes, checksum: a2477e790479eb126430056ae6e07301 (MD5) Previous issue date: 2016-07-28 / Essa pesquisa pretende descrever compreensivamente os sentidos de ser (trans) educadora social. A educação ocorre em diversas situações e de variadas maneiras e, por isso, a população excluída encontra, em espaços diferentes da escola, práticas e ações que, de alguma forma, contribuem para o enfrentamento das desigualdades. Esses movimentos pedagógicos estão inseridos na pedagogia social que é uma ciência que tem como foco a promoção da educabilidade de pessoas e grupos que se encontram em condição de desigualdade. Nesse sentido, a população trans, em muitas situações, sofre com a rejeição familiar e comunitária, transformando a rua em destino e local privilegiado para construção de suas identidades e bandeiras de resistência, em uma sociedade que as impede de ter acesso aos direitos sociais mais básicos. Para construção deste trabalho foi adotada a perspectiva qualitativa e utilizado como inspiração o método fenomenológico-existencial, tendo como referência teórica as contribuições de Paulo Freire. A captura dos dados aconteceu a partir da história oral e de vida que estimou a realização de três entrevistas não-diretivas possibilitando a livre expressão de uma trans educadora selecionada sobre seus modos de ser. O Ser (trans) educadora social está intimamente ligado ao compromisso com a dignidade de seus pares, da família e de colegas que também experimentaram a exclusão na escola. Ser (trans) educadora social se desvela no apego à fé, quando o único destino é a rua onde o medo é uma sensação muito presente. O Ser (trans) educadora social produz uma pedagogia do aprender com as cicatrizes das travestis mais experientes, na produção do corpo e na construção propostas e projetos que levem cidadania e alegria para essa população. O Ser (trans) educadora social está envolvido no resistir à desumanização promovida pelas drogas, pela prostituição, pelas relações com aliciadores e cafetões e por um modelo educacional que não aceita as diferenças e impõe a evasão. Ser (trans) educadora social é, fundamentalmente, construir uma educação no chão da rua, firmada em ser-com-o-outro, adaptada à realidade vivida pela população trans e que busca o ser mais, transformando as rebeldias cotidianas em ações revolucionárias.

No description available.

Rational drug design is a powerful method in which new and innovative therapeutics can be designed based on knowledge of the biological target aiming to provide more efficacious and responsible therapeutics. Understanding aspects of the targeted biological agent is important to optimize drug design and preemptively design to slow or avoid drug resistance. Chagas disease, an endemic disease for South and Central America and Mexico is caused by Trypanosoma cruzi, a protozoan parasite known to consist of six separate genetic clusters or DTUs (discrete typing units). Chagas disease therapeutics are problematic and a call for new therapeutics is widespread. Many researchers are working to use rational drug design for developing Chagas drugs and one potential target that receives a lot of attention is the T. cruzi trans-sialidase protein. Trans-sialidase is a nuclear gene that has been shown to be associated with virulence. In T. cruzi, trans-sialidase (TcTS) codes for a protein that catalyzes the transfer of sialic acid from a mammalian host coating the parasitic surface membrane to avoid immuno-detection. Variance in disease pathology depends somewhat on T. cruzi DTU, as well, there is considerable genetic variation within DTUs. However, the role of TcTS in pathology variance among and within DTU’s is not well understood despite numerous studies of TcTS. These previous studies include determining the crystalline structure of TcTS as well as the TS protein structure in other trypanosomes where the enzyme is often inactive. However, no study has examined the role of natural selection in genetic variation in TcTS. In order to understand the role of natural selection in TcTS DNA sequence and protein variation, we sequenced 540 bp of the TcTS gene from 48 insect vectors. Because all 48 sequences had multiple polymorphic bases, we examined cloned sequences from two of the insect vectors. The data are analyzed to understand the role of natural selection in shaping genetic variation in TcTS and interpreted in light of the possible role of TcTS as a drug target.

Within the next decade an inter provincial trail, called the Trans Canada Trail, will be developed to accommodate non-motorized traffic such as hikers, skiers, horsemen, and cyclists. Anticipating the need for rest stop facilities, this thesis seeks to explore the design potential of two isolated facilities. The focus of this exploration is the impact of landscape on the development of architectural form. While existing conditions found in each site were recorded and considered throughout the design process, a conscious effort was also made to build a site rather than site plan This position, first articulated by William Rees Morrish in his book Civilizing Terrain. acknowledges that not every piece of land can become an urban place. Instead, placemaking often requires willful acts of change in order to enhance or more effectively reveal its existing qualities. This position opposes the current attitude fostered by the environmental movement that all landscapes should remain untouched. The two sites selected were both located in western Canada: one on the eastern shore of Lake in southern British Columbia and the other in a farmer's field near Milk River in southern Alberta. This thesis traces the discoveries and attempts made to locate a center, develop an arrival sequence and insert an architectural form into the experience of the landscape. Both sites were explored simultaneously, reaching a similar level of resolution, at which point the British Columbia site was dropped and the prairie site was developed further. The Alberta site then became the developed body of the thesis. / Applied Science, Faculty of / Architecture and Landscape Architecture (SALA), School of / Graduate

This thesis challenges common assumptions of trans moving images by applying theories of identification to an autoethnographic close reading of three specific texts – Hedwig and The Angry Inch (John Cameron Mitchell, 2001), Dallas Buyers Club (Jean- Marc Vallée, 2013) and Transparent (Jill Soloway, 2014) - considered by both mainstream and queer audiences to feature transgender characters and experiences. This thesis, while limited to the author’s experience as a trans woman, attempts to advance the argument that identification with trans moving images may change with one’s transition and require a reassessing of “what is trans” along with resituating the trans spectator from “object of the gaze” to “bearer of the look” (Mulvey, 1975).

When not invisibilized in society, transgender women are subject to pervasive transphobia in the social sphere and encounter devastating discrimination across the institutions. In light of discrimination in the mainstream job market, many look to the sex industry for a source of income. In fact, trans women have long ties to the sex work community and have been foundational to the sex workers rights movement, engaging in activism predating Stonewall. Yet, the experiences of trans women who sell sex remain largely overlooked in historical retellings and social science research on sex work. By creating space for the voices of those who have long gone unheard – conducting in-depth interviews with seven transgender women who sell sex – this thesis seeks to move beyond the dominant narrative of sex workers and bring greater attention to their unique experiences. This thesis explores the experiences of trans women in sex work as it relates to their labour practices and processes, engagement with the criminal justice system, and health and access to health and social services. Challenging the ways in which norms have been produced and sustained under the guise of ‘truth’, this thesis applies the concepts intersectionality and stigma to explore how experience is conditioned by the environment of oppression – at the intersection of sexism and cisgenderism – and the social judgment and marginalization of sex workers. Alongside navigating through the hardships associated with the criminalization and stigmatization of sex work, trans women who sell sex are subject to intensified violence, discrimination and oppression on the basis of gender. Amidst the height of the trans rights movement and in light of the recent amendments to federal legislation that protects trans Canadians from discrimination, this thesis considers what is warranted to achieve social change. Reflecting on history and looking to the future, it is imperative that the trans and sex worker communities forge stronger bonds in their battle for rights.

Memoria para optar al título de Ingeniero en Alimentos / Se ha estudiado la aplicación del proceso de interesterificación enzimática, con la finalidad de desarrollar un sistema que permita la obtención a escala industrial de bases grasas alternativas, que contengan un bajo porcentaje de ácidos grasos trans (AGT). Las materias primas utilizadas fueron mezclas de aceite de pescado “full” hidrogenado (AFHP) con aceite vegetal líquido (AVL) para la mezcla con base pescado, y aceite vegetal “full” hidrogenado (AFHV) con AVL para la mezcla con base vegetal. El catalizador empleado fue una lipasa 1,3 específica al 4% y 70 ºC. Las variables estudiadas fueron: % de materia prima en la mezcla (AFHP o AFHV) y tiempo de reacción. El efecto se midió sobre las variables punto de fusión (PF) y contenido de sólidos grasos (CSG) a diferentes temperaturas. Se aplicó un diseño factorial bidimensional en tres niveles, 32, como diseño de optimización del proceso, encontrándose como resultado un tiempo de reacción de 5 horas (tiempo máximo) para ambas mezclas y una proporción de 43,9% para AFHP en la mezcla con base pescado y 36,8% para AFHV en la mezcla con base vegetal. Se establecieron los modelos matemáticos para PF y CSG a diferentes temperaturas. Se determinó el contenido de AGT mediante IR y GLC, estructura cristalina por microscopía de luz polarizada, características texturales mediante análisis de compresión y cambios en el contenido de triglicéridos para la base vegetal mediante HPLC / The enzymatic interesterification process has been applied with the purpose to develop a system for obtaining alternative fatty bases with low percentage of trans fatty acids (AGT) for industrial production. The raw materials used were full hydrogenated fish oil (AFHP) and liquid vegetal oil (AVL) for the fish blend and full hydrogenated vegetal oil (AFHV) with AVL for the vegetal blend. The catalyst used was 4% 1,3 specific lipase, temperature 70 ºC. The variables studied were: % of raw material in the blend (AFHP or AFHV) and reaction time. The effect was measured by melting point variable (PF) and solid-fat content (CSG) at different temperatures. A 3 levels factorial bidimensional design, 32, for process of optimization was applied. The results were maximum reaction time 5 hours for both mixtures and a proportion of 43.9% for AFHP in the fish base mixture and 36.8% for AFHV in the vegetal base mixture. The mathematical models for the PF and CSG at different temperatures were established. AGT, crystalline structure, textural characteristics and changes in the triacylglicerol content for the vegetal blend were determined by IR and GLC, polarized light microscopy, compression analysis and HPLC, respectively

No description available.

This paper is about the representations of the female body in Sylvia Plath’s The Bell Jar. The pure female body, the sexual female body and motherhood (the female body as a mother) are analysed through an ecofeminist perspective. The way the bodies are represented describe much more than what is on the surface, and through an ecofeminist perspective the reader can understand what is said beyond the words. The female body will also, at times, be analysed in correlation with its relationship with nature in order to understand the way the protagonist, Esther Greenwood views other female bodies. This analysis will lend itself to the reader to further understand Sylvia Plath’s protagonist and how Plath uniquely represents female bodies and the characters in charge of their female bodies.