The aim of this thesis is to analyse word order in transitive sentences in natural production of two- and four-year old children during Czech language acquisition. The theoretical part focuses on language acquisition in general, in children in a given age period and on the acquisition of transitive sentences in children, discussed in studies carried out abroad. The results of these studies work with a thesis about a canonical word order, which is the first to emerge in child production and reception, while other types of word order might pose a problem for children with regards to interpretation. In order to be able to compare these findings with Czech language, data from an existing corpora of child conversations with their mothers were coded for the purposes of the practical part of this thesis. This data is subsequently analysed in the practical part, with the findings of these analyses implying that in spontaneous language production of Czech children, it is impossible to unambiguously track the preferences for canonical word order, as well as difficulties in adopting word order other than canonical. This thesis also brings basic descriptive information about the word order in transitive sentences both in children over time and in relation to word order that is used by their mothers. Key words...