Design of frame of a human powered vehicle (HPV) is the aim of this diploma thesis. Wide analysis precedes design process. The thesis describes historical developement of land transportation, especially history of cycling, bicycles and other human povered vehicles, which are not so extended. Preferences and deficiencies of transportation by these ecological vehicles are shown. The thesis includes summary of most widespread and best-known conceptions, including their advantages and disadvanteges. Conception of three-wheeled vehicle was selected. It has two steered front wheels and one powered rear wheel. The accent is putted on wide efficiency, ergonomics, simplicity of vehicle`s frame and possibility of using common cycling components. 3D model of frame and assembly of vehicle was created. This model was checked by FEM computation.