The diploma project Circulation tries to solve the problem of repeating cycles. It is an object with organic shape, created from a tube with light circulation on it. This object is based on idea of human constantly repeating actions and processes, whether they refer to the human body and mind, nature or society. The object is created site-specifically in to the gallery (Brno Gallery OFF/FORMAT) and tries to fill the space. The line passing through the gallery space can be seen as a route or path, but it can also remind a movement without a clear goal, or go around in circles. The omnipresent cycle, is then an individual contribution to the whole and it can be presented as a irregular curve full of dramatic twists and turns, ups and downs. The light (made by digital LED strip), which constantly copies the organic shape of the tube, represents an endless flow of information. The object tries to point out the never ending circulations in our lives; the object does not solve the problem, it is rather a statement.

The majority of research on high strain-rate effects in timber structures has been limited to the study of the load-bearing members in isolation. Limited work has been conducted on timber connections and full-scale timber assemblies under blast loading, and these have generally been constrained to qualitative observations. In North America, the increasing prevalence of mid- and high-rise timber structures makes them susceptible to blast effects. In addition, questions remain on how to design and optimize these timber assemblies, including the connections, against blast loads, due in part to the limitations on comprehensive design provisions. The effects of far-field blast explosions were simulated using the University of Ottawa shock tube. A total of fifty-eight dynamic tests were conducted on connection-level and full-scale specimens. The research program aimed to investigate the behaviour of heavy-timber connections when subjected to simulated blast loads. The experimental results showed that connections with a main failure mechanism consisting of wood crushing experienced significant increases in dynamic peak load when compared to the static peak load. In contrast, connections where steel yielding and rupturing occurred experienced no statistically significant increase in dynamic peak load. Full-scale glulam specimens with bolted connections designed to yield via wood crushing and bolt bending performed better than those with overdesigned connections. Bolted connections which failed in splitting led to premature failure of the glulam assembly. Reinforcement with self-tapping screws allowed these bolted joints to fail in a combination of bolt yielding and wood crushing, and provided more ductility when compared to unreinforced specimens. Specially designed energy-absorbing connections significantly increased the energy dissipation capabilities of the timber assemblies. The basis of these connections was to allow for connection yielding while delaying failure of the wood member. This was achieved via elastoplastic connection behaviour, which effectively limited the load imparted onto the wood member. Based on the experimental results, limitations in the current Canadian blast provisions were highlighted and discussed. A two-degree-of-freedom blast analysis software was developed and validated using full-scale and connection-level experimental results and was found to adequately capture the system response with reasonable accuracy. Sensitivity analyses regarding the applicability of using single-degree-of-freedom analysis were presented and discussed.

In order to increase the efficiency of a preheating system as temperatures increase, without using seawater as a coolant, an additional heat exchanger is required to be installed into the system. The purpose of the study is therefore to investigate how the temperatures of fuel oils can be increased while minimising the loss of energy and costs. The study was conducted in Karlshamn Power Station's (kvt) preheating system on Block 3. The study was conducted via Design for six sigma (DFSS) according to the primary design concept SG2 to guarantee the study's understanding of empirical data, and the development of solutions. The study resulted in two variants of heat exchangers where one is an identical Shell and Tube heat exchanger from Siljan Allards AB and the other one is a Supermax Shell and Plate heat exchanger from Tranter International.

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We study the influence of finite shear deformations on the microstructure and rheology of solutions of entangled semiflexible polymers theoretically and by numerical simulations and experiments with filamentous actin. Based on the tube model of semiflexible polymers, we predict that large finite shear deformations strongly affect the average tube width and curvature, thereby exciting considerable restoring stresses. In contrast, the associated shear alignment is moderate, with little impact on the average tube parameters, and thus expected to be long-lived and detectable after cessation of shear. Similarly, topologically preserved hairpin configurations are predicted to leave a long-lived fingerprint in the shape of the distributions of tube widths and curvatures. Our numerical and experimental data support the theory.

This dissertation thesis summarizes results of experiments that have been carried out during my PhD studies related to the new mass spectrometric methods for trace gas analysis of human breath. The thesis is divided into the theoretical and experimental part. The chapter at the beginning of this dissertation summarizes the current research in the area of breath analysis. It is describing the common breath metabolites, benefits and challenges of the method for therapeutic monitoring and clinical diagnosis and current applications. The next chapter of the theoretical introduction describes the techniques suitable for this area of research, with a special emphasis on mass-spectrometric techniques (in particular the selected ion flow tube mass spectrometry, SIFT-MS, method that allows accurate quantification of trace gases and vapours in humid air/human breath). All these parts are elaborated via the scientific literature review. The following chapters are then directly related to my own research and describes the conducted experiment, including the results obtained. This experimental part "Results and Discussion" is divided to the individual subsections, which are conceived as the commentaries to the enclosed research papers published in peer reviewed journals. The first is the detailed step by step...

This diploma thesis deals with the topic of nutritional support in patients with neurological diseases (stroke, craniocerebral trauma, critical-illness-polyneuropathy. The aim of this thesis is to find out, how implemented unified system of nutritional support affects the well-being of patients at neuro-rehabilitation clinic Asklepios Schlossberg Klinikum in Bad König. There were observed 58 patients (33 men and 25 women) during 8 weeks. The theoretical part of this thesis describes basic components of nutrition, energy expenditure and needs, selected neurological diseases, dysphagia, malnutrition and nutrition in intensive neurological care. The practical part of the thesis analyses data obtained by the observation. There are observed changes of body weight, BMI and laboratory values of total protein and albumin in serum. These data are divided by sex, age and type of disease. Based on the results, it was found that although patients due to uniform tube feeding do not have sufficient protein intake, serum total protein and albumin levels increased. This can be caused by eating a uniform diet that contains good quality protein in patients, which suffered from hypoalbunemia at the outset of observation, also by reducing the effect of stress reactions with gradual improvement of the state and...

The phase-out of ozone-depleting refrigerants, such as R-12 and R-22, according to the Montreal Protocol of 1987, has provided the incentive to increase the thermal efficiency of current heating and refrigeration systems. The purpose of this study was to increase the accuracy of the predictions of both the heat transfer and pressure drop correlations for condensing refrigerants in the Intermittent flow regime. This was done utilizing a novel method involving the temporal and spectral analysis of the light intensity of the local flow regime, as seen through a sight glass. An experimental setup was designed, built and commissioned specifically for this purpose using refrigerant R-22 and a smooth tube. It was found that the accuracy of the mean heat transfer coefficient predictions increased substantially compared to other leading correlations,particularly at low mass fluxes. In terms of the pressure drop,the predictions also increased in accuracy, and it was found that the time fraction method allows for continuous predictions over flow regime transitions when using local flow-pattern-based pressure drop models. This was previously not possible. / Dissertation (MEng (Mechanical Engineering))--University of Pretoria, 2007. / Mechanical and Aeronautical Engineering / unrestricted

Laser absorption based sensors are extensively used in a variety of gas sensing areas such as combustion, atmospheric research, human breath analysis, and high resolution infrared spectroscopy. Quantum cascade lasers have recently emerged as high resolution, high power laser sources operating in mid infrared region and can have wide tunability range. These devices provide an opportunity to access stronger fundamental and combination vibrational bands located in mid infrared region than previously accessible weaker overtone vibrational bands located in near infrared region. Spectroscopic region near 8 µm contains strong vibrational bands of methane, acetylene, hydrogen peroxide, water vapor and nitrous oxide. These molecules have important applications in a wide range of applications. This thesis presents studies pertaining to spectroscopy and combustion applications. Advancements in combustion research are imperative to achieve lower emissions and higher efficiency in practical combustion devices such as gas turbines and engines. Accurate chemical kinetic models are critical to achieve predictive models which contain several thousand reactions and hundreds of species. These models need highly reliable experimental data for validation and improvements. Shock tubes are ideal devices to obtain such information. A shock tube is a homogenous, nearly constant volume, constant pressure, adiabatic and 0-D reactor. In combination with laser absorption sensors, shock tubes can be used to measure reaction rates and species time histories of several intermediates and products formed during pyrolysis and oxidation of fuels. This work describes measurement of the decomposition rate of hydrogen peroxide which is an important intermediate species controlling reactivity of combustion system in the intermediate temperature range. Spectroscopic parameters (linestrengths, broadening coefficients and temperature dependent coefficients) are determined for various transitions of acetylene. Furthermore, methane and acetylene sensors are developed for shock tube applications. The application of these sensors (along with an ethylene sensor) has been demonstrated to measure these species during the pyrolysis of n-pentane and iso-pentane.

Nitric oxide (NO) is an important signaling molecule in plants. In the pollen of Arabidopsis thaliana, NO causes re-orientation of the growing tube and this response is mediated by 3′,5′-cyclic guanosine monophosphate (cGMP). However, in plants, NO-sensors have remained somewhat elusive. Here, the findings of an NO-binding candidate, Arabidopsis thaliana DIACYLGLYCEROL KINASE 4 (ATDGK4; AT5G57690) is presented. In addition to the annotated diacylglycerol kinase domain, this molecule also harbors a predicted heme-NO/oxygen (H-NOX) binding site and a guanylyl cyclase (GC) catalytic domain which have been identified based on the alignment of functionally conserved amino acid residues across species. A 3D model of the molecule was constructed, and from which the locations of the kinase catalytic center, the ATP-binding site, the GC and H-NOX domains were estimated. Docking of ATP to the kinase catalytic center was also modeled. The recombinant ATDGK4 demonstrated kinase activity in vitro, catalyzing the ATP-dependent conversion of sn-1,2-diacylglycerol (DAG) to phosphatidic acid (PA). This activity was inhibited by the mammalian DAG kinase inhibitor R59949 and importantly also by the NO donors diethylamine NONOate (DEA NONOate) and sodium nitroprusside (SNP). Recombinant ATDGK4 also has GC activity in vitro, catalyzing the conversion of guanosine-5'-triphosphate (GTP) to cGMP. The catalytic domains of ATDGK4 kinase and GC may be independently regulated since the kinase but not the GC, was inhibited by NO while Ca2+ only stimulates the GC. It is likely that the DAG kinase product, PA, causes the release of Ca2+ from the intracellular stores and Ca2+ in turn activates the GC domain of ATDGK4 through a feedback mechanism. Analysis of publicly available microarray data has revealed that ATDGK4 is highly expressed in the pollen. Here, the pollen tubes of mis-expressing atdgk4 recorded slower growth rates than the wild-type (Col-0) and importantly, they showed altered NO responses. Specifically, the mis-expressing atdgk4 pollen tubes have growth rates that were less affected by NO and showed reduced bending angles when challenged by an NO source. Further works on atdgk4 knockout/knockdown mutants will reveal the biological functions of ATDGK4 in NO and/or cGMP signaling in the pollen, and in the broader fertilization process.