Diss.--Zürich--Philosophische Fakultät 1, 1987. / Bibliogr. p. 239-246.

The proteolytic enzymes in the visceral organs of albacore tuna fish (Thunnus alalunga) were studied, initially as an extract of the whole viscera, then as extracts of the individual organs. Preliminary studies indicated three pH optima of activity in the whole viscera extracts pH 1.7, 3.1-3.5 and 9.5. Analysis of extracts of the individual organs revealed the alkaline proteolytic activity was present in the intestine and the pyloric caeca, with the pyloric caeca showing the highest activity. The stomach extract demonstrated high protease activity at pH 1.7 and 3.5, while the remaining organs had relative activities at least ten times lower at these pH values. The extract obtained from pyloric caeca was studied in more detail. The approximate levels of trypsin-1ike and chymotrypsin-like activities were measured, and the effects of three inhibitors, phenylmethylsulfonylfluoride (PMSF), l-chloro-3-tosylamido-7-amino-L-2-heptanone (TLCK) and L-1-tosylamide-2-phenylethylchloromethylketone (TPCK), determined. Cationic discontinuous polyacrylamide gel electrophoresis coupled with a substrate inclusion technique for localization of the separated proteases of the pyloric caeca extract revealed the presence of ten proteolytic enzymes. One was active in acid solution (ca. pH 4) and was inhibited by PMSF and TLCK. The remaining nine proteases were active at pH 8.0. Five of the alkaline proteases were serine proteases and one of these showed trypsin-like specificity including PMSF and TLCK inhibition and activity against BANA. None of the electrophoretically separated proteases showed specificities similar to chymotrypsin. / Graduation date: 1976

Thesis (Ph. D.)--University of California, San Diego, 1998. / Vita. Includes bibliographical references (p. 129-130).

Thesis (M.C.M.) -- Lincoln University, 2009. / Also available via the World Wide Web.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1984. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 186-198).

The tunas could be called the 'ultimate teleosts'. They have a high percentage of muscle and a high percentage of that muscle is red muscle, the muscles are kept at above ambient temperatures by a counter current heat exchanger and the respiratory capabilities of these fish are accordingly high. The behavioral culmination of these characteristics is manifested in swimming speeds, which can be extraordinarily high, on a sustained, or a burst basis. One of the hottest and fastest tunas, the skipjack, was used in a study to determine (1) when each muscle is active (2) when and where the muscle heat is produced and (3) what the advantage of the hot musculature is to the animal. Evidence from E. H. , histological, enzyme and metabolite studies suggest that the red muscle is qualitatively quite typical although its aerobic capacity is somewhat above that of other teleost red muscles. The white muscle has truely astounding anaerobic capabilities, but also displays an aerobic capacity not usually found in teleost white muscle. Further examination of white muscle biochemical organization revealed a GP cycle which balances redox during the aerobic catabolism of glycogen and/or glucose. Both LDH (the terminal step in anaerobic glycolysis) and GPDH (the cytoplasmic arm of the GP cycle) are present in white muscle in high activities. Since these enzymes potentially compete for a common co-substrate, NADH, a tight control of these two enzymes seemed necessary to ensure mutual exclusive activity. Metabolite regulators of both enzymes were found which by affecting the ability of each enzyme to compete for NADH, channel carbon and hydrogen to lactate and C02 and H20 under anaerobic and aerobic conditions respectively. , The effect of temperature on metabolism was investigated and it is concluded that the stability rather than the absolute 'set point' of the body temperature is the more important feature. / Science, Faculty of / Zoology, Department of / Graduate

Typescript. / Thesis (Ph. D.)--University of Hawaii, 1969. / Bibliography: leaves [136]-142. / ix, 142 l

Typescript. Includes vita and abstract. Includes bibliographical references (leaves 303-330). Also available online in Scholars' Bank; and in ProQuest, free to University of Oregon users.

Traditionally, it is commonly believe that the marine fish stock is renewable and inexhaustible. However, following a dramatic progress of fishing technology, it is proved that the unregulated fishing activity would cause the resource stock deteriorated. It is a necessary evil to design a management procedure, which implements an annual fishing quota system in order to maintain the sustainability of the fish resource. In the most recent years, sudden implementation quota restrictions in many international commissions for management and conservation of marine mammal and fish had posted a challenge to the fishing company of the Far Sea Fishery in Taiwan, which was not used to the quota regulation before. In the short run, the fishing company would react to get more quotas in any necessary ways to ensure their profitability, even though the quota is not obtained in a regular way. Five conservation commissions in the three oceans for the conservation of Tuna and Tuna-like Species have been established. International Commission for the Conversation of Atlantic Tuna (ICCAT) is the organization of Atlantic. Western and Central Pacific Fisheries Commission¡]WCPFC¡^and Inter-American Tropical Tuna Commission¡]IATTC¡^is the organization of Pacific. The organization of Indian Ocean is Indian Ocean Tuna Commission¡]IOTC¡^which is a sub-organization of FAO(Food and Agriculture Organization of the United Nations). Commission for the Conservation of Southern Bluefin Tuna¡]CCSBT¡^is a organization covering three oceans. Three commissions have implemented annual quota among the five. In recent year, the quantity of our fishing vessels have overtaken Japan and become the top rank of the Deep Sea Longline Fishing country in the world. Though there are still 2 commissions do not implement quota restriction, it is believed to be implemented soon. When the quota restriction is implemented by all the commissions, the fishing company can not get additional quota any more, then it will be very difficult to operate by a normal way. In order to avoid the serious influence of insufficient quota, the operating strategy must be made as soon as possibly. First of all, the basic concept of the Ultra Low Temperature Longline Fishery is introduced. Then the background and quota condition of the five tuna fish conservation commissions will be mentioned, especially the Indian Ocean will be discussed with more details. The case of fishing company will be taken as an example for the research. Except the detailed analysis of the case, some related good management will be considered also. A suggestion how to adjust the operation to facing the insufficiency of quota will be proposed as a reference. Keyword¡GTuna, Tuna Conservation, Tuna Long Line Fishing, Tuna Quota, SWOT Analysis, Fishery Management Strategy

Thesis (Ph. D.)--University of Tennessee, Knoxville, 2002. / Title from title page screen (viewed Oct. 8, 2002). Thesis advisor: Matthew N. Murray. Document formatted into pages (x, 158 p. : ill. (some col.)). Vita. Includes bibliographical references (p. 151-157).