With continuously increasing urbanization, consumer demands and expansion of water supply systems, determination of efficient pump schedules became a more difficult task. Pumping energy costs constitute a significant part of the operational cost of the water distribution networks. This study aims to provide an effective daily pump schedule by minimizing the energy costs for constant and also for multi tariff of electricity (3 Kademeli Elektrik Tarifesi) in water distribution network. A case study has been performed in an area covering N8.3 and N7 pressure zones which are parts of Ankara water distribution network. Both pressure zones consists of 3 multiple pumps in pump station and one tank having 5000 m3 storage volume each. By using genetic algorithm based software (WaterCAD Darwin Scheduler) least-cost pump scheduling and operation policy for each pump station has been determined while satisfying target hydraulic performance requirements such as minimum and maximum service pressures, final water level of storage tank and maximum velocity in pipeline. 32 different alternative scenarios have been created which include multi tariff energy prices, constant tariff energy price, insulated system condition, uninsulated system condition and different pump combinations. The existing base scenario and alternative scenarios which were prepared by using optimal pump schedules have been compared and the achievements of optimizing pump operation have been analyzed. At the end of the study, a satisfying result has been observed that by using determined optimal pump schedule, minimum % 14 of total energy cost can be saved in existing water supply system.