Diante da necessidade de responder algumas indagações relacionadas a epidemiologia da Estomatite Vesicular, principalmente aquelas que dizem respeito a ocorrência de surtos em locais onde existem coleções d\'água, foi desenvolvido um modelo de transmissão do VSA utilizando a água como via de transmissão, a tilápia nilótica, inoculada intraperitonealmente, como fonte de infecção e o cobaio como hospedeiro susceptível. O objetivo da utilização deste modelo biológico de transmissão do Vírus da Estomatite Vesicular foi de avaliar o papel desempenhado pelos peixes no ciclo epidemiológico, propor um modelo de ciclo epidemiológico do VSA, destacando o papel da água como via de transmissão e padronizar uma técnica de RT-PCR para a detecção do VSA, em amostra de tecidos. Através do modelo desenvolvido, fica demonstrado que estes peixes eliminaram partículas virais na água, decorridos 13 dias pós-inoculação e que esta última se caracteriza como via de transmissão, possibilitando a infecção dos hospedeiros susceptíveis (cobaios) através de inoculações experimentais em coxim plantar. A tilápia nilótica pode ser considerada como uma fonte de infecção, por ser capaz de eliminar um agente infeccioso no meio ambiente e através de uma via de transmissão este agente alcançou o hospedeiro susceptível; os peixes podem ser inseridos no ciclo epidemiológico da Estomatite Vesicular como fonte de infecção, sendo capazes de eliminar na água partículas virais infectantes, destacando o papel da água como via de transmissão; fica padronizada uma técnica de RT-PCR dirigida ao gene codificador da proteína RNA-polimerase, útil para a detecção direta do Vírus da Estomatite Vesicular Alagoas e Indiana em amostras de tecidos. / A model of transmission of Vesicular Stomatitis was developed to Vesicular Stomatitis Alagoas (VSA) serotype employing water as a way of transmission, the Nile tilapia intraperitoneal inoculated as a source of infection and guinea pigs as susceptible hosts aiming to answer many questions concerning Vesicular Estomatitis epidemiology, as the risk of disease on farms with dose relationship with riverine areas and the role of fishes in the epidemiological cycle of the disease. Furthermore, a RT-PCR assay was developed to detect VSA in tissue samples. According to the experimental transmission, fishes eliminated virus into the water after 13 days pos-infection and a model to VSA epidemiological cycle is proposed in which water was characterized as a way of transmission, carrying the virus to the susceptible host through experimental inoculation and the Nile tilapia should be thought as a source of infection, once it was able to eliminate the infective agent into the environment. A useful tool to the diagnosis of both Indiana and Alagoas serotypes was developed.

Thesis (M. Sc.(Agronomy)) -- University of Limpopo, 2017. / Cleome gynandra is increasingly becoming an important strategy for achieving food and nutrition security among rural households in many developing countries. Root-knot (Meloidogyne species) nematodes, with limited nematode management strategies, limit the successful production of this vegetable crop. Nemafric-BL and Nemarioc-AL phytonematicides are separately being developed in South Africa for sustainable crop production systems. However, the two products have not been simultaneously tested for managing the notorious Meloidogyne species and absorption of phosphorus, with a combination of Vesicular arbuscular mycorrhiza (VAM). The objective of this study therefore was to determine the interactive effects of VAM and each of the two phytonematicides on nutrient content, growth of C. gynandra. A 2 × 2 × 2 factorial experiment, with the first, second and third factors being VAM (V), nematode (N) and Nemafric-BL phytonematicide (P). The eight treatments included (1) untreated control (V0N0P0), (2) nematodes alone (V0N1P0), (3) VAM alone (V1N0P0) (4) Nemarioc-AL phytonematicide alone (V0N0P1), (5) V1N1P0, (6) V0N1P1, (7) V1N0P1 and (8) V1N1P1, were laid out in a randomised complete block design, with ten replications. The same layout experiment was done for the Nemarioc-AL phytonematicide trial which had a similar layout. Seedlings were irrigated with 250 ml chloride-free tapwater every other day for 56 days. Multifeed and NPK (2:3:2(22) fertilisers were applied at transplanting. The second order interaction (V1N1P1), was highly significant (P ≤ 0.01) for plant height contributing 54% in TTV (Total Treatment Variation) of the variable. Among the main factors (N, P and V), only nematode had highly significant effects on stem diameter. All interactions of VAM, nematode and Nemarioc-AL phytonematicide and main factors each had no significant effect on Cleome. The second order (V1N1P1) and the first order interaction (V1N1P1) did not have significant effects on the three nutrient elements except for the first order interaction (V1N0P1) which was significant on foliar Zn contributing 42% in TTV of the variable. Also nematode had highly significant effect on foliar K and significant effect on foliar Zn contributing 49 and 31% in TTV of the respective variables. Using the two-way table, VAM and Nemafric-BL phytonematicide each increased foliar Zn by 27% and 29%, respectively. The second and first order interactions of VAM, N and Nemarioc-AL phytonematicide and the main factors did not have significant effect on foliar K, Fe and Zn. The second order interaction of VAM, nematode and Nemafric-BL phytonematicide had significant effects on gall rating, contributing 2% in TTV of the variable. VAM, nematode and Nemarioc-AL phytonematicide showed that the second and first order interaction except for V1N0P1 interaction on gall rating, were not significant for nematode variables. The V1N0P1 interaction contributed 20% in TTV of gall rating. Using a two-way table, VAM and phytonematicide each increased root galls by 7% and 74%, respectively. Combined, VAM and phytonematicide reduced root galls by 64%. The innovative products interacted together and that Nemafric-BL and Nemarioc-AL phytonematicides and VAM alone could be used in managing nematodes. / National Research Foundation, Agricultural Research Council-Universities Collaboration Centre

No description available.

Arbuscular mycorrhizal (AM) fungi form an obligate symbiosis with many wild and cultivated plants. The plant may benefit from improved nutrient uptake (particularly phosphorus) and resistance to drought, pests and disease. Compared to more natural habitats, arable systems support reduced diversity of AM fungi, with community structure shifted in favour of ruderal types. Physical disturbance is an important driver of these differences and reduced tillage systems might facilitate greater utilisation of the AM symbiosis as part of more sustainable production systems. In this study, the structure of root-associated AM fungal communities in barley grown under a range of tillage regimes was characterised, using high-throughput molecular methodology. AM fungal community structure was significantly influenced by tillage intensity, with soils subject to high tillage burden richest in ruderal types. These findings were consistent in both winter and spring barley cultivars. Significant temporal changes in AM fungal community structure suggested an important role for plant growth stage in determining AM fungal community dynamics. Functional differences can occur between fungal species and different fungus-plant combinations, and in the second part of this study the functional consequences of changes in community structure due to tillage were tested in a model system using intact field-soil cores. There were clear differences in AM fungal community structure due to differential physical disturbance. Although no functional effects were observed, this may have been due to the inherent limitations of recreating field conditions in microcosm experiments. The results of this comprehensive assessment of fine-scale spatial variation in AM fungal community structure in an arable system identify tillage as an important driver of AM fungal community dynamics, and plant growth stage is also a key factor which has rarely been addressed. Elucidating the functional significance of these changes remains essential in order to justify future changes to arable management practices.

Identifying the mechanisms that prevent competitive exclusion in tropical forests is a key goal of tropical ecology. Because trees are long-lived organisms, it is complicated to test theory related to coexistence. However, the seedling stage, during which tree mortality is the highest, offers an ideal proxy to evaluate mechanisms that promote or hinder tree species coexistence. This dissertation utilizes both theory and empirical approaches to investigate two mechanisms thought to influence seedling dynamics and tree species coexistence: negative feedbacks from tree natural enemies and positive feedbacks from arbuscular mycorrhizal (AM) fungi. Tree natural enemies might promote tropical tree species coexistence by acting as agents of negative density-dependent mortality. Simultaneously, tropical seedlings associate with arbuscular mycorrhizal (AM) fungi, which can increase seedling growth and survival through enhanced nutrient and water uptake. However, little is known about the effects of AM fungi on tropical tree community dynamics. In chapter 1, I developed a theoretical model that combines competition between trees, predation by tree natural enemies, and mutualism with AM fungi, and showed that a subtle balance between negative and positive feedbacks is required to reach tree species coexistence. In chapters 2 and 3, I used empirical data collected from El Yunque (Puerto Rico) to gain a better understanding of the distribution of soil fungi and tree natural enemies in a secondary tropical forest and to test some of the assumptions of my theoretical model. In chapter 2, I found evidence that soil characteristics and the tree community were important to structure soil fungal communities, and I demonstrated long-lasting effects of past human land use. If AM fungi are important to promote tropical tree species coexistence as suggested by my theoretical model, past land use could influence tree species coexistence by altering AM fungal communities, emphasizing the need for additional studies about land use legacy effects on AM fungal communities. In chapter 3, I showed that seedlings at intermediate conspecific density and from intermediate abundance tree species, hosted the richest community of natural enemies, suggesting that negative density-dependent processes might be non-linear, and partially supporting my theoretical model. Finally in chapter 4, I investigated seedling mortality and showed that natural enemies increase seedling mortality, whereas AM fungal diversity decreases seedling mortality, counteracting the local effects of natural enemies. I also found evidence that AM fungal diversity rescues rare tree species, and natural enemies reduce survivorship of more abundant species, thereby preventing competitive exclusion. Therefore, at the community scale, AM fungal diversity and natural enemies act in the same direction, promoting tropical tree species coexistence, which is consistent with the findings of my theoretical model. In conclusion, this dissertation jointly investigated the effects of negative and positive feedbacks on tropical tree species coexistence, and demonstrated the importance of combining demographic processes that are known to occur simultaneously.

No description available.

Copies of author's previously published articles inserted. / Bibliography: leaves 184-197. / xxvi, 197 leaves, [5] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Soil Science, 1995

Bibliography: leaves 146-170. / xix, 170 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--Dept. of Soil and Water, Waite Agricultural Research Institute, University of Adelaide, 1994

The overall objective of the work presented in this thesis was to investigate roles of arbuscular mycorrhizal ( AM ) fungi in growth and phosphorus ( P ) nutrition of wheat ( Triticum aestivum L. ) in a highly calcareous soil from the Eyre Peninsula, South Australia. The soil used for this study is one of the main soil types used for wheat production in South Australia. It is severely P - deficient, but plant responses to conventional fertiliser application are poor. Although the total P and Colwell - extractable P contents of the soil are high, the resin - extractable P content is very low. Resin - extractable P is better able to predict P availability for plant growth than Colwell - extractable P. The soil is also strongly P - fixing. Moderate levels ( about 20 mg kg [superscript minus 1] ) of resin - extractable P for wheat could only be achieved by adding high rates ( up to 100 mg kg [superscript minus 1] ) of CaHPO4 in this soil. A bioassay with wheat showed that it can be highly colonised by AM fungi in the soil. AM fungi have been shown to improve P nutrition of plants, particularly in nutrient poor soils. They may thus be important for wheat grown in the soil with low amounts of plant - available P such as the one used. The first part of the work involved conventional pot experiments. Effects of AM fungi on wheat were compared between sterilised soil and non - sterile soil, sterilised soil inoculated with non - sterile soil or with Glomus intraradices or noninoculated, with different soil / sand mixes. Colonisation of wheat at 8 weeks was high, with about 75 % of root length colonised for indigenous fungi and 55 % for Glomus intraradices, regardless of the soil treatments. Growth and P uptake of wheat were significantly increased by both indigenous fungi and G. intraradices, irrespective of soil / sand mixes. Effects of indigenous fungi on plant growth were larger in sterilised and inoculated soil than in non - sterile soil. In sterilised soil, increases of plant growth by AM fungi were higher with G. intraradices than with indigenous fungi. Dilution of the soil by mixing with sand reduced plant growth and P uptake of both AM and non-mycorrhizal ( NM ) plants. In another experiment, responses of wheat to AM fungi and P supply were compared with those of clover. Plants were inoculated with four different AM fungi. Colonisation of wheat was lower than clover. Although suffering from P deficiency, NM wheat ( 6 weeks ) grew relatively well with no added P ( P0 ) and application of P at 100 mg kg [superscript minus 1] ( P100 ) increased the dry weight ( DW ). Shoot P concentrations increased with P application and there were positive effects of all AM fungi at P100. In contrast, NM clover ( 8 weeks ) grew very poorly at P0 and did not respond to P application. Clover responded positively to all AM fungi at both P levels, associated with increases in P uptake. The results showed that responses of wheat to AM inoculation and P supply were quite different from those of clover, and emphasized the different abilities of the two species to access P in the very high P - fixing soil used. Responses of two wheat cultivars ( Brookton and Krichauff ) to AM fungus ( G. intraradices ) were also evaluated with different P supplies at two developmental stages ( vegetative and maturity ). Colonisation by G. intraradices of both cultivars was well established at 6 weeks ( ~ 50 % in P0 plants ) and continued to increase up to maturity ( ~ 70 % ), but decreased greatly at both harvests as P supply was increased ( up to 150 mg P kg [superscript minus 1] ). Addition of P significantly increased plant growth, grain yield and P uptake irrespective of cultivar and harvest time, and the optimum soil P for grain yield was 100 mg kg [superscript minus 1]. In both cultivars, a growth depression in AM plants occurred at 6 weeks at all P levels, but this disappeared at 19 weeks with added P. At P0, AM plants produced lower grain yield per plant, but with higher P supply, AM plants produced higher grain yields than NM plants. There was a significant positive effect of AM on grain P concentration at P0, but not at other P levels. Brookton was somewhat more P efficient than Krichauff, and the latter responded more to AM fungi. The results showed that responses of wheat to AM fungi and P supply changed during development. Growth depression induced by AM fungi in low P soil was overcome by addition of moderate amounts of P, resulting in significant increases in grain yield in AM plants. Additional approaches were used to help determine the roles of AM fungi in wheat growth and nutrition. The effects of plant density were tested, as it was expected that increasing density might decrease the negative effects of AM fungi on wheat growth. Large growth depressions were induced by both G. intraradices and Gigaspora margarita in wheat grown at low density, although % colonisation by G. intraradices was higher than by Gi. margarita. With increasing plant density, the growth depressions were smaller, indicating that competition modulates growth responses. Although there may be effects due to competition for soil P, it is clear that with increasing plant biomass per unit soil volume, the AM fungal biomass did not increase in proportion ; in fact, hyphal length density decreased. Accordingly, costs of AM in terms of organic carbon loss per plant decreased with increasing plant density, thus mitigating the growth depression. The results add to the increasing body of evidence that mycorrhizal growth responses of plants grown singly may not apply at the population or community level as in crops. Two compartmented pot systems were used to examine whether the fungal hyphae deliver the P into the plants even in the absence of positive growth responses. An experiment in which plants were constricted in a mesh bag, but hyphae of AM fungi could explore a large soil volume was carried out. Results suggested that AM fungi helped the plants acquire P, although mesh bags did not remove AM growth depression. The experiments in which AM fungi were supplied with [superscript 32]P in a small soil compartment to which only hyphae had access showed that a considerable amount of P was delivered to wheat plants via AM fungi. The original aim was to calculate the percentage of total P entering the plants via the AM pathway. However, realistic values were not obtained probably because of difficulties of determining plant-available P and uneven distribution of hyphae in the soil. It is also possible that plants and AM fungi access different P pools. This study demonstrated the potential roles of AM fungi in growth and P nutrition of wheat grown in the highly calcareous soil from the Eyre Peninsula, South Australia. Further studies on the effects of the interactions between AM fungi and wheat in the field are needed to assess the contribution of AM fungi to plant nutrition. / Thesis (Ph.D.)--School of Earth and Environmental Sciences, 2005.

Copies of author's previously published articles inserted. Bibliography: leaves 184-197.