Since the White River in east central Indiana is a source of water for human use, the quality of its water is an important concern. Certain heavy metals, when present in high enough concentrations may cause discoloration, foul taste, or may even be a health hazard to man. The particular metals of interest in this investigation are iron, copper, mercury, lead, chromium, nickel, and cobalt. The present study is concerned with the measurement of the concentrations of these metals in the waters and sediment of the White River at Muncie, Indiana.The analyses for the metals listed were performed via appropriate standard methods, involving principally atomic absorption spectroscopy. Four river sites in the Muncie vicinity were sampled daily for a. week, one week per month, for two months, using techniques designed both for convenience and representative character of samples.The end-products of this study are a, comparison of the heavy metal concentrations in the White River with values from other studies and a discussion of the many variables needed to present a true picture of the metallic content of the river water.

This study is an investigation into the biodegradation potential of a TCE contaminated aquifer located at the Atomic Weapons Establishment in the UK, and the remediation prospects by employing biological and biotechnological processes such as bacterial community structure and diversity assessment, toxicity and isotopic fractionation.  Quarterly monitoring of boreholes from the former military site suggested that the plume is predominantly aerobic and therefore reliance cannot be placed only on natural attenuation.  In addition the contaminated plume contains a variety of volatile organic chemicals, petroleum, diesel and metals, which introduces potential co-metabolites as well as additional toxicity.  Assessment of the natural bacterial community of the site revealed sufficient bacterial counts to support intrinsic bioremediation and the presence of multiple additional contaminants.  Variable toxicity responses not solely explainable by TCE were observed with a broad functional bacterial diversity, which could be bio-stimulated (with substrates such as the terpenes carvone, pulegone and linalool as well as the aromatic compound cumene) or bio-augmented (with <i>Dehalosporillium multivorans</i>) to increase rates of TCE degradation.  Other additional agents such as iron filings were able to stimulate rapid rates of TCE degradation.  There is also evidence of potential TCE degraders within the more heavily contaminated boreholes (the finding of <i>Pseudomonas putida</i>).  Lastly isotopic fractionation suggests that degradation of TCE is occurring on site.

M.Sc. (Zoology) / Please refer to full text to view abstract

A thorough biological survey and evaluation is a lengthy and expensive project. The number of technically trained persons required prevent its use by most public agencies. Since public health departments are the groups most frequently concerned with measuring the effects of pollution in streams, a need exists for a simplified method of sanitary survey. Recognizing this need, the Texas Health Department assigned the writer the problem of devising a method of survey which would require a field party of two and could be conducted entirely from a mobile field laboratory.

The Problem of the cause and control of tastes and odors in water supplies has been the subject of a great many investigations during the last half-century; however, many questions in this field remain to be answered. Theories relating to the cause of tastes and odors occurring in water are still rather numerous and, at present, only a few of them are compatible. Although several procedures have been employed for the eradication of tastes and odors from water supplies, none of them are completely successful.

Fens are increasingly recognised as important habitats in terms of biodiversity, and this has been formalised in recent legislation (EC Habitats Directive, 92/43/EEC). The influence of groundwater and surface water inputs on the fen habitat increases its vulnerability to water pollution, particularly from nutrients. Despite the conservation importance and potential vulnerability of the habitat, fens have not been widely studied in Scotland, in terms of extent, location, sensitivity to increased nutrient inputs, or in comparison to similar European sites. This study found that fens were widespread throughout mainland Scotland, and that basin fens were the most commonly occurring fen type, representing 43% of the 355 sites assessed. A survey of 18 basin fens found that 72% were potentially vulnerable to elevated nutrient inputs, being surrounded by improved/modified land. Despite this, only four sites showed greater nutrient concentrations in sub-surface water samples or water inputs. There was, however, a significant amount of variation in hydrochemistry between the sites. Detailed studies further assessed spatial and temporal variations in hydrochemistry, and associated hydrologic regimes on two similar basin fens, one in an agricultural catchment, the other surrounded by unimproved grassland. Spatial patterning of vegetation was associated with both base-richness and nutrient concentrations of subsurface water, and the frequency of water inundation. General relationships between the six fen vegetation types and the observed hydrological and hydrochemical variation were presented.

M.A. / South Africa is situated within a semi-arid part of the world which is characterised by high seasonal variability in terms of rainfall and runoff, with high evaporation rates. This causes streamflow to be relatively low for most of the year, with seasonal sporadic high flows. Further stress is applied to the water resource through population growth, increased urbanisation and industrial activities. The study area is considered to be the most populated of the Upper Vaal Water Management Area (WMA), which is the most important WMA in terms of economic productivity in South Africa. This research report focused on assessing the temporal and spatial variations of pollution between four different sampling points located in the Vaal Barrage Reservoir, which is located in the heart of the Upper Vaal WMA. The Vaal River Barrage Reservoir forms a 64 kilometres long water body with an estimated total storage capacity of 63 million litres of water. The four sampling points are V2 (Vaal River at Engelbrechts Drift Weir); VRB 24 (Vaal River Barrage at 24 km); VRB 37 (Vaal River Barrage at 37 km) and V17 (Vaal Barrage Reservoir Outlet). The aim of this research is to determine the type of physical and chemical pollutants within the Vaal River Barrage which currently poses the biggest problem to river health. The spatial and temporal differences of the pollutant loads are established and discussed. In addition, it is determined what the seasonal influence of rainfall has on the water quality measured at the four different sampling points. Chemical pollutants which currently pose the biggest threat in terms of water quality for the Vaal Barrage Reservoir are Phosphates, Electrical Conductivity and Sulphates. These three water quality variables do not comply with the minimum standards as set by Rand Water. The occurrence of these pollutants in the Vaal River can be explained by the vast inputs of return flow water from sewage treatment plants, underground mine water and discharge from industries. Microbiological factors were not taken into consideration for this dissertation, due to the unavailability of the data for most of the sampling sites for the majority of the study period. It was found that there are seasonal variations in terms of water quality at three sampling points: VRB24, VRB37 and V17. Sample point V17 had the highest inverse correlation for the three pollutants which pose the biggest threat to the health of the Vaal Barrage Reservoir water body. This implies that high seasonal variability occurs in the pollutant load at this sampling point. Sample point V2 had extremely low inverse correlation figures, which implies that rainfall has little or no impact on the level/concentration of a pollutant. This can be explained by two factors. Firstly the dilution effect which water released from the Vaal Dam has due to the close proximity to V2. Secondly because urban, mining and industrial activities are much less evident at this point, and subsequently return flows are less.

M.Sc. / Please refer to full text to view abstract

M.Sc. / Please refer to full text to view abstract

A thesis submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Doctor of Philosophy Johannesburg 2012 / Wastes from mining operations usually contain a suite of pollutants, among them cyanide and its complexes; heavy metals; metalloids and radionuclides. The pollution plume can affect public health through contamination of drinking water supplies, aquatic ecosystems and agricultural soils. As such, waste management and remediation has become an important integral component of mining. Conventional chemical and physical methods are often expensive and ineffective when the pollutant concentrations are very high, so the challenge of developing cost-effective materials with high adsorption efficiencies for pollutants still remains. This research was dedicated to the development of biosorbents with high metal loading capacity for the remediation of mine wastewater, namely: zeolite/bentonite functionalised with microbial components such as histidine, cysteine, sorbitol and mannitol; zeolite/bentonite functionalised with Penicillium-simplicissimum and zeolite-alginate complex generated by impregnating natural zeolite into alginate gel beads. The ability of the fresh water algae, Oedogonium sp. to remove heavy metals from aqueous solutions in batch systems was also assessed. Optimum biosorption conditions for the removal of Co, Cu, Cr, Fe, Hg, Ni, Zn and U (in a single-ion and multi-ion systems) were determined as a function of pH, initial concentration, contact time, temperature, and mass of biosorbent. An increase of adsorption capacity was observed following modification of natural zeolite/bentonite by microbial components with a maximum adsorption capacity obtained at low pH. The FTIR results of the developed biosorbents showed that the biomass has different functional groups that are able to react with metal ions in aqueous solution. Immobilisation of fungi (Penicillium-simplicissimum) on zeolite/bentonite yielded biomass of 600 mg g-1 (10-fold higher than the non-immobilised one) at a pH 4, showing the potential of this sorbent towards remediation of AMD-polluted mine sites. The maximum uptake of metals ions (in a multi-ion system) was higher and constant (40-50 mg g-1) in the inactive fungal biomass (heat-killed) from pH 2 to 7. The uptake of U and Hg increased significantly in the zeolite/bentonite-P.simplicissimum compared to their natural forms due to the presence of the N-H, S-H and COO- groups. iii The pseudo second-order adsorption model was found to be more suitable in describing the adsorption kinetics of metal ions onto biomasses in single- and multi-ion systems with the sorption of nickel being controlled by film diffusion processes (with the coefficient values of 10-7 cm2 s-1). The thermodynamic parameters showed that the adsorption onto developed biosorbents was feasible and spontaneous under the studied conditions. The calculated values of the loading capacities in column adsorption for the natural zeolite/bentonite as well as zeolite/bentonite-P.simplicissimum were close to those obtained in the batch tests, mainly for U and Ni. The bed depth service time model (BDST) was used successfully to fit the experimental data for Ni and U adsorbed on the natural zeolite. This suggested a linear relationship between bed depth and service time, which could be used for scale-up purpose. The developed biosorbents could be regenerated using 1 mol L-1 HNO3 solution for potential re-use. The total decrease in biosorption efficiency of zeolite-Penicillium simplicissimum after five cycles of adsorption-desorption was ≤ 5% which showed that zeolite/bentonite-Penicillium simplicissimum had good potential to adsorb metal ions repeatedly from aqueous solution. On applying it to real wastewater samples, the zeolite-P. simplicissimum biosorbent removed 97% of the metals. Penicillium sp. immobilisation enhanced the potential and makes it an attractive bioremediation agent. The zeolite-alginate sorbent exhibited elevated adsorption capacities for metals. This showed potential for use of such a system for remediation purposes. It also provides a platform to explore the possibility of using zeolite in conjunction with other polysaccharide-containing materials for heavy metal removal from wastewaters. The results obtained in this study have shown that zeolite and bentonite are good supports for biomass. The biofunctionalised zeolite/bentonite systems have potential in removal of heavy metals from wastewaters.