This thesis discusses the use of mycorrhizal fungi in nursery production. In the literature review there is summarized current knowledge regarding mycorrhiza and nursery production of ornamental plants. As a model plant for the experiment was used Spiraea bumalda "Anthony Waterer" and the experiment was conducted in the grounds of The Department of Breeding and Propagation in Lednice. There was applied mycorrhizal product Symbivit when planting in batch 6 g, 3 g, 1,5 g and 0 g. The plants were evaluated by parameters as plant height, diameter of root collar, the number of shoots. Then there were evaluated mycorrhizal colonization and volumes of roots balls in the laboratory. The experiment was described and evaluated statistically. The experiment resulted in the fact, that the more mycorrhizal product Symbivit is applied, the greater was the colonization. The mycorrhizal product had a positive effect on plant growth. The plants treated with product Symbivit in batch greater than 3 g proved a statistically significant difference in the monitored parameters compared to the control. There is also a economic viewpoint, the price of plants increases after the application of mycorrhizal product Simbivit.