The exposure of cells to a wide range of stressors results in a highly
synchronized, genetically determined response, initiated by detection of the
stressor, which in turn leads to a regulatory response that involves the elevated
synthesis of a specific set of proteins, termed heat shock proteins (HSPs), which
serve to counteract the initial damage,and re-establish cellular homeostasis. The
expression of HSP30, 60, 70, and 90 was measured in the heart, liver, kidney, lung,
and gonads of Japanese quail exposed to seven different stressors (mild restraint,
loud noise, inescapable irritation, cold temperature, isolation in darkness and two
stressful social situations), and in the heart, liver, kidney and lung of non-diabetic
and diabetic rats exposed to seven individual stressors. Tonic Immobility (Tl) tests
were also conducted on Japanese quail to assess whether or not the stressors
increased fear response. Increased expression of HSP70 was found in the heart
tissue of birds exposed to loud noise, inescapable irritation, cold temperature and
isolation in darkness. The expression of other HSPs was not apparent in the heart
or any of the other tissues examined. Longer Tl was observed only in birds
exposed to the noise stress. Increased expression of HSP30 and HSP70 was found
in the heart tissue of non-diabetic, stressed rats. Increased expression of HSP30
and HSP70 was also found in acute (4 weeks) and chronic (12 weeks)
streptozotocin rats. In the liver, increased HSP70 expression was found for acute and chronic diabetic treatments. In the kidney, increased HSP70 expression was
found only in chronic diabetes. No change in HSP 60 or 90 was detected in any
tissues examined and the lung did not show any HSP increase. These results
support the possibility of a tissue- and class-specific HSP response when exposed
to a variety of stressors. / Land and Food Systems, Faculty of / Graduate
| Identifer | oai:union.ndltd.org:UBC/oai:circle.library.ubc.ca:2429/6325 |
| Date | 11 1900 |
| Creators | Hoekstra, Kenneth Andrew |
| Source Sets | University of British Columbia |
| Language | English |
| Detected Language | English |
| Type | Text, Thesis/Dissertation |
| Format | 6019157 bytes, application/pdf |
| Rights | For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use. |
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