Serotonin receptor subtypes 5-HT1A, 5-HT2A and 5-HT2C are expressed in motoneurons and modulate motoneuron excitability. Serotonergic neurons, which increase their discharge with motor activity, make numerous contacts with motoneurons; however, little is known about the adaptability of motoneuron serotonin receptor expression in response to exercise. The purpose of this study was to determine the effect of a 7-day treadmill exercise protocol on 5-HT1A, 5-HT2A and 5-HT2C receptor mRNA levels in rat lumbar motoneurons. Lumbar motoneurons of exercised and sedentary animals were collected via laser capture microdissection. RNA was isolated from these samples and real-time reverse transcription polymerase chain reactions were performed to determine differences in receptor mRNA levels between exercised and sedentary animals. It appears that 5-HT1A, 5-HT2A and 5-HT2C receptor mRNA levels are unaltered following 7 days of treadmill exercise; however, future research must be done to determine if an exercise effect exists when motoneurons are differentiated by type.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:MWU.1993/4178 |
| Date | 12 September 2010 |
| Creators | Woodrow, Lindsey |
| Contributors | Gardiner, Phillip (Kinesiology and Recreation Management), Duhamel, Todd (Kinesiology and Recreation Management) Jordan, Larry (Physiology) |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_US |
| Detected Language | English |
Page generated in 0.0018 seconds