碩士 / 靜宜大學 / 食品營養研究所 / 98 / Tinnitus is a phantom sensation of sound in the absence of external stimulations while its pathogenesis is unclear and effective treatment has not been developed yet. To approach the phantom sensation, we aimed to develop a SAMP8 mice behavioral model of conscious tinnitus using salicylate, an active component of aspirin known to induce tinnitus. The purpose of this study was to examine the effects of Spirulina and C-phycocyanin on tinnitus in senescence accelerated prone mice (SAMP8). Three-month-old SAMP8 mice were randomly divided into four experimental groups, including (A) normal control, (B) tinnitus group with intraperitoneal injection of salicylate (300 mg/Kg BW), (C) oral Spiulina (1000 mg/Kg BW) with salicylate-induced tinnitus group and (D) oral C-PC (130 mg/Kg BW) with salicylate-induced tinnitus group. Body weight and active avoidance test were performed during nine days of feeding period. The tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), cycoloxygenase-2 (COX-2) and N-methyl-D-aspartate receptor 2B (NMDA receptor 2B) mRNA expressions of brain stem, hippocampus and inner ear were examined after sacrificed. The results showed that the salicylate-induced tinnitus group led to a decrease in the percentage of correct response (score) and a drastic increase in the number of false positive response (i.e., animals execute the motor task during a silent period), and Spirulina or C-PC with salicylate-induced tinnitus groups had significantly improved, indicated that the Spirulina and C-PC could improve the learning and memory impairment and the conscious tinnitus. Salicylate inhibited the cyclooxygenase-2 (COX-2) mRNA expression in hippocampus and inner ear, and Spirulina and C-PC also decreased the COX-2 mRNA expression. When compared with salicylate-induced tinntus group, both Spirulina and C-PC could lower the TNF-α and IL-1β mRNA over-expression of brain stem, hippocampus and inner ear after salicylate injection. Spirulina and C-PC also could decrease the NMDA receptor 2B mRNA over-expression in hippocampus and inner ear. Furthermore, C-PC treatment should more effective than the Spirulina treatment group. In summary, we conclude that the supplementation of Spirulina and C-PC could improve the salicylate-induced conscious tinnitus in SAMP8 mice. The mechanism maybe related to the over-expression of TNF-α, IL-1β, NMDA receptor 2B. The variation of different tissues maybe related to the part of induced tinnitus after salicylate injection.
| Identifer | oai:union.ndltd.org:TW/098PU005255010 |
| Date | January 2010 |
| Creators | Shan-ying Yang, 楊珊盈 |
| Contributors | Yin-ching Chan, 詹吟菁 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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