碩士 / 靜宜大學 / 食品營養研究所 / 99 / Salicylates, also known as aspirin, is known to induce tinnitus. However, the mechanism of generation of tinnitus induced by salicylate is still unclear. Tinnitus is a non-observable phantom sensation, and had no effective treatment until now. Spirulina platensis showed good antioxidant capacity, anti-inflammation and neuroprotective effect. The purpose of this study was to examine the effects of Spirulina platensis on salicylate-induced tinnitus in senescence accelerated prone mice (SAMP8), and evaluated the related gene expression of antioxidative, anti-inflammatory and ion channels. Three-month- old SAMP8 mice were randomly divided into three experimental groups, including normal control, tinnitus group with intrperitoneal injection of salicylate (300 mg/Kg BW), and Spirulina platensis (1000 mg/Kg BW) with salicylate injection tinnitus groups. The active avoidance test were performed during the last nine days of feeding period. After sacrificed, the serum biochemical parameters were analyzed and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and Heme oxygenase-1 (HO-1) 、K+-Cl- cotransporter 2 (KCC2)、Na+-K+-2Cl- cotransporter 1(NKCC1)、5-hydroxytryptamine 2B recrptor (5-HT2B receptor) mRNA expsression of brainstem, hippocampus, temporal lobe, frontal lobe and inner ear were examined. The results showed that correct response score did not significantly different after salicylate injection while the number of false positive response was drastic increase, and spirulina platensis administration could improve the conscious tinnitus. There were not significant differences on GOT, GPT, BUN and creatinine levels among three groups. Salicylate inhibited the CAT mRNA expression and induced the over-expression of SOD mRNA in brain stem, hippocampus, temporal lobe, frontal lobe and inner ear. Spirulina platensis supplementation could increase the CAT mRNA expression and lower the SOD mRNA over-expression when compare with the salicylate only group. Spirulina platensis also could decrease the KCC2 mRNA over-expression in hippocampus, temporal lobe, frontal lobe and inner ear, whereas the difference did not found in the area brain stem. However, there were not significant differences on the GPx , HO-1, NKCC1 and 5-HT2B receptor mRNA expression among three groups. In summary, we concluded that Spirulina platensis could improve the salicylate-induced conscious tinnitus in SAMP8 mice. The mechanism might relate to promote the antioxidantive defense system and reduce the over-expression of KCC2 mRNA.
| Identifer | oai:union.ndltd.org:TW/099PU005255006 |
| Date | January 2011 |
| Creators | Nian-cih Chang, 張念慈 |
| Contributors | Yin-ching Chen, 詹吟菁 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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