碩士 / 國立臺北護理健康大學 / 聽語障礙科學研究所 / 101 / Tinnitus can be associated with hearing impairment Ginkgo Bilobalide (GIKO) is a popular herbal remedy for the prescribed treatment of tinnitus. However, there appears to be a strong placebo effect in tinnitus management. The present study aims to examine how GIKO could affect the salicylate (SA)-induced tinnitus in animal rat model. Functional behavior and the associated c-fos and glutamate decarboxylase (GAD) changes in the inferior colliculus of these GIKO-treated control or tinnitus rats were concurrently evaluated.
Rats were divided into 4 groups: two control groups- rats with oral saline (NS + NS, n = 4) or rats with oral GIKO (NS + GIKO), and two experimental groups (SA induced tinnitus rats)-rats with oral saline (SA + NS) or with oral GIKO (SA + GIKO). Analysis of tinnitus behaviors with between-group design, rats in the control group reached 80% of correct response; however, no significant difference was found within the control groups (NS vs. GIKO, p = .4895). During the tinnitus induction and treatment periods, rats in the experimental groups reached 70% of correctness rate, however, there was no significant difference within groups (NS vs. GIKO, p = .1714) in the induced period. Interestingly, the experimental and control groups were found to be significantly different, SA + NS relative to the NS + NS (p = .0419 *) and NS + GIKO (p = .020 *). The experiment group in the treatment period with between-group design, SA+NS and the SA+GIKO had no significant difference (p = .5584), the control group and experiment group within subject design also found no significant difference.
Comparing the false positive behavioral responses, no significant difference was found in rats in the control groups (NS vs GIKO, p = 0.784). By contrast, GIKO significantly reduced SA-induced behavior in the experimental group (NS vs GIKO; p = .0377*). This indicates a protective effect of GIKO on tinnitus induction. In four group between- group design, the experiment groups were more significantly different than the control groups, by comparing SA + NS with NS + NS ( p = .0123*) and with NS + GIKO ( p = .0123*)，SA + GIKO with NS + NS ( p = .0002***) and with NS + GIKO ( p = .0002***). The experiment groups were analyzed with within-subject design and there was found to be significant difference (p = .0313*).
Immunohistochemical staining results of c-fos and GAD in rat inferior colliculus showed that there was no significant difference of c-fos immunoreactivity (IR) in 4 groups of rats. Interestingly, GAD –IR in GIKO-treated rats were significantly higher than that in NS within the control groups (p = .0054**).Furthermore, there was also a trend of increase GAD-IR in GIKO than in NS within the experiment groups although this difference did not reach statistical significance (p = .342).
Taken together, the present work employed SA-induced tinnitus rat model and demonstrated beneficial effectiveness of GIKO on the false positive behavioral responses and the associated GABA transmission (GAD-IR) in the inferior colliculus. This study also highlights SA-induced tinnitus rat model as a good research platform.
| Identifer | oai:union.ndltd.org:TW/101NTCN0714018 |
| Date | January 2013 |
| Creators | Kai- Hunh Hung, 洪愷鴻 |
| Contributors | Chii-Yuan Huang, 黃啟原 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 55 |
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