碩士 / 國立交通大學 / 分子醫學與生物工程研究所 / 102 / Tinnitus is a phantom perception of sound in the absence of external sound stimulation. It is the most prevalent disorder of hearing. Unfortunately, no effective cure is currently available because of its diverse causes and clinical presentations and its underlying neural mechanisms are still poorly understood. SS-induced rat is one of widely used tinnitus animal models. The SS-induced tinnitus was associated with over-activity in the central auditory pathways, e.g., auditory cortex. Whether the lower efferent relays (e.g. lateral superior olivary complex, LSO) are involved remains unclear.
In this study, we used Fos-immunohistochemistry to map the over-active neurons at the auditory pathways following a single dose of SS injection. Specifically, we examined the cochlear nucleus (CN), superior olivary complex (SOC), inferior colliculus (IC), medial geniculate body (MGB) and auditory cortex (AC).
Experimental rats (n = 12) received single of SS (250 mg/kg, i.p.). Rats were sacrificed at 2 hours after the injection. Standard Fos-immunohistochemical staining procedures were used for processing frozen sections (40 µm in thickness). Control rats received saline injections.
Results showed that following the SS injection there was a significant increase (p < 0.05) in the number of Fos-stains at the LSO and AC. In contrast, only small number of IC neurons expressed Fos after SS injections. MGB neurons no expressed Fos after SS injections.
Results of an increase of Fos-immunoreactivity at LSO and AC suggested that the pathogenesis of SS-induced tinnitus involves an alteration in the efferent mediated feedback systems such as AC and LSO. However, how AC and LSO neurons may change their activity in a temporal manner and the details of regulation mechanisms during the pathogenesis of SS-induced tinnitus would obviously require a time-lapse study in the future.
| Identifer | oai:union.ndltd.org:TW/102NCTU5538013 |
| Date | January 2014 |
| Creators | Chen, Yu-Shan, 陳玉珊 |
| Contributors | Chiu, Tzai-Wen, 曲在雯 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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