碩士 / 明新科技大學 / 化學工程與材料科技系碩士班 / 103 / Armillariella mellea is one of the fungi food-medicine mushroom with the effects of tranquilizing central nervous and reducing death rate of mice inducing by nicotine. Simultaneously, it can treat dizziness, palsy, insomnia, tinnitus, etc. Many researchers demonstrated that A. mellea polysaccharide has excellent anti-tumor activies. Recently, although many researchers focus on activity components and fermentation products as well as development in health-food, little literature was reported concerning effect of plant oils and Vitamins supplement on production and insulinotropic activity of polysaccharides of A. mellea by submerged culture. Thus, the project was proposed to investigate the effect of plant oils and vitamins on the production of polysaccharide in submerged culture of A. mellea and their antioxidant properties. Amongst the various plant oils and vitamins tested, 0.2% (w/v) Thiamine hydrochloride (Vit.B1 . HCl) and 0.2% (v/v) Cotton oil exhibited the greatest biomass and exopolysaccharoide production. In 5 L stirred tank bioreactor culture, adding 0.2% (v/v) cotton oil to medium can obtain the maximum biomass and exopolysaccharide production with 7.41 gL-1及0.43 mg mL-1, respectively. Adding 0.2% (w/v) Vit.B1 . HCl to medium can obtain the maximum biomass and exopolysaccharide production with 17.9 gL-1及0.41 mgmL-1, respectively. Besides, in 20 L stirred tank bioreactor culture, adding 0.2% (v/v) Cotton oil to medium also can obtain the maximum biomass and exopolysaccharide production with 9.01 gL-1 and 0.46 mgmL-1, respectively. Four fractions (EPS-A, EPS-B, EPS-C, and EPS-D) of exopolysaccharide isolated from 20 L stirred tank bioreactor culture show 1.28×104 Da, 1.36×104 Da, 6.82×103 Da, and 1×104 Da of molecular weights, respectively. Molecular weight of exopolysaccharide from 20L stirred tank bioreactor culture is 2.31×106 Da. The protein/polysaccharide ratios of four fractions (EPS-A, EPS-B, EPS-C, and EPS-D) are 28.21%, 27.04%, 27.39%, and 28.09%, respectively. The protein/polysaccharide ratio of exopolysaccharide from 20L stirred tank bioreactor culture is 28.32%. Antioxidant activity results of four fractions (EPS-A, EPS-B, EPS-C, and EPS-D) point out that EPS-A has a higher reducing powers with 0.008 mgmL-1 of EC50, EPS-B has better scavenging effects on 1,1-diphenol-2-picrylhydrazyl radicals with 0.15 mgmL-1 of EC50, EPS-C shows the low EC50 values of chelating effects of 1.74 mgmL-1. The exopolysaccharide from 20L stirred tank bioreactor culture exhibited a high antioxidant activity and scavenging effects on superoxide anions with EC50 values of 0.6 mgmL-1 and 0.56 mgmL-1, respectively. EC50 values of EPS-D are below 8.64 mgmL-1 indicating good antioxidant activities. Because all EC50 are below 10 mgmL-1 except scavenging effects on superoxide anions of EPS-A and EPS-B with EC50 values of 16.14 mgmL-1 and 11.58 mgmL-1, respectively, polysaccharides from A. mellea submerged culture are of exceptional antioxidant activities.
| Identifer | oai:union.ndltd.org:TW/103MHIT0063004 |
| Date | January 2015 |
| Creators | Wan-Yi Wan, 萬琬儀 |
| Contributors | Ming-Yu Lung, 龍明有 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 107 |
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