The diploma thesis summarizes the experimental method for the rearing of zebrafish Danio rerio (Hamilton, 1822) screed. The work of figuring out which of the methods of rearing fry in the transitional period of the ontogenetic development is best for the survival rate and growth of the standard length of the surveyed individuals.
The test was carried out on two basic lines of zebrafish Wild Type and Casper. At the beginning of the test were created four groups divided by feeding mode. The control group was fed once a day with pellet feed GEMMA Micro 75 and one day from the 10th day after fertilization the fed with nauplii stages of brine shrimps Artemia salina (Linnaeus, 1758). The second group was fed only once a day with pellet feed GEMMA Micro 75. The last two groups were fed five times a day saltwater rotifer species Brachionus plicatilis (Müller, 1786), and only one of them and the other was still three times daily nourished with nauplii stages of brine shrimps. For each group of four representative have been created initially, thirty tanks of fish at the age of five days after fertilization.
The results of this method have shown that the methods they use to feed the rotifers, did not have a statistically significant impact on the survival rate of individuals. In the growth of this method has proven to be statistically significant result in the group where the fed in combination with brine shrimps, where to achieve the average standard size of juvenile fish 16.02 +/- 0.80 mm for the Wild type and 17.39 +/- 0.81 mm for Casper, compared to the control group, that has been the standard length 11.63 +/- 0.64 mm for the Wild Type and 9.54 +/- 0.56 mm for Casper.
This method has great potential and breeding is therefore necessary to further develop this method and to adapt it to individual zebrafish facilities.
| Identifer | oai:union.ndltd.org:nusl.cz/oai:invenio.nusl.cz:257712 |
| Date | January 2016 |
| Creators | Gottwald, Milan |
| Contributors | Kalous, Lukáš, Martin, Martin |
| Publisher | Česká zemědělská univerzita v Praze |
| Source Sets | Czech ETDs |
| Language | Czech |
| Detected Language | English |
| Type | info:eu-repo/semantics/masterThesis |
| Rights | info:eu-repo/semantics/restrictedAccess |
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