In this study, 59 epidemiologically unrelated Salmonella strains isolated from foods
in Tü / rkiye and 49 Salmonella strains obtained from National Salmonella Reference
Laboratories of Germany were analysed. For the characterization of strains, analyses
such as serotyping, phage typing, antibiotyping and molecular biological
characterization were done. The strains exhibited 17 different serotypes with S.
Enteritidis serotype and PT21 phage type being the most prevalent in Turkish
isolates. The highest antimicrobial resistance was observed against NAL for Turkish
strains, whereas it was against SUL for strains from German origin. Molecular typing
of all strains exhibited different plasmid profiles and PFGE patterns. There were
1-4 plasmids/profile for Turkish strains and 1-7 plasmids/profile for German strains.
The PFGE patterns revealed 42 different subgroups, having two major clusters with
44,3% arbitrary homology. Among 72 resistant strains, the most prevalent resistance
genotypes were observed as blatem-1 (%56, AMP resistance) / floR (%100, CHL and
FFC resistance) / aphA1 (%100, KAN and NEO resistance) / tet(A) (%53, TET
resistance) / aadA1 (%82, SPE and STR resistance) / sulI (%78, SUL resistance). The
class I integron variable region analyses exhibited 700 bp (1 strain), 1000 bp (37
strain), 1200 bp (16 strain) and 1600 bp (3 strain) integrons.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12608844/index.pdf |
| Date | 01 September 2007 |
| Creators | Avsaroglu, M. Dilek |
| Contributors | Bozoglu, Faruk |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | Ph.D. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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