The interaction of recombinant TR with TRE-containing double-stranded DNA duplexes was monitored using the electrophoretic shift assay (EMSA). A protein titration allowed the calculation of the K& of TR for DNA. This quantitation of the affinity of TR for DNA was subsequently measured in the presence of known T3 analogues, thus providing the basis of a TR-DNA binding assay.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:583735 |
| Date | January 2006 |
| Creators | Thomas, Gethin Owain |
| Publisher | Cardiff University |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Source | http://orca.cf.ac.uk/54105/ |
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