Several genes thought to confer susceptibility to dyslexia have been identified, and the purpose of this study is to 1) determine the expression pattern of one of these gene products and 2) characterize the function of the product of one of these genes, namely KIAA0319-Like (KIAA0319L), using the developing chick visual system as a
model.
Whole mount in situ hybridization was performed for KIAA0319L on embryonic
day (E)3 – E5 and in situ hybridization on sections was performed at later stages.
Engineered microRNA (miRNA) constructs targeting KIAA0319L were prepared and
their specificity and efficiency for knocking down KIAA0319L were tested. miRNAs
were electroporated in E5 optic tecta (OT). Embryos were sacrificed at E12. OT were
removed, sectioned and analyzed.
Results demonstrate that KIAA0319L is expressed in the developing chick visual
system. Knockdown of KIAA0319L in the OT results in abnormal migration indicating
that KIAA0319L is necessary for this process.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/29509 |
| Date | 23 August 2011 |
| Creators | Charish, Jason |
| Contributors | Monnier, Philippe |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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