Wong, Kai Yi. / Thesis (M.D.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 101-110). / Abstracts in English and Chinese. / Abstract --- p.4 / 摘要 --- p.7 / Acknowledgements --- p.9 / List of Tables --- p.10 / List of Figures --- p.11 / List of Abbreviations and Symbols --- p.12 / Chapter Chapter 1 --- Introduction --- p.14 / Chapter 1.1 --- Staphylococcus aureus --- p.14 / Chapter 1.2 --- Methicillin-resistant Staphylococcus aureus (MRSA) --- p.14 / Chapter 1.2.1 --- Methicillin resistance of MRSA --- p.15 / Chapter 1.2.2 --- Staphylococcal Chromosomal Cassette mec (SCCmec) --- p.16 / Chapter 1.2.3 --- Hospital-associated MRSA (HA-MRSA) and Community-associated MRSA (CA-MRSA) --- p.22 / Chapter 1.2.3.1 --- Hospital-associated MRSA (HA-MRSA) --- p.23 / Chapter 1.2.3.2 --- Community-associated MRSA (CA-MRSA) --- p.23 / Chapter 1.2.4 --- Pathogenesis of MRSA infection --- p.27 / Chapter 1.2.4.1 --- Possible virulence genes contributing to necrotizing pneumonia --- p.29 / Chapter 1.2.4.1.1 --- Panton-Valentine Leukocidin (PVL) --- p.29 / Chapter 1.2.4.1.2 --- Phenol-soluble modulins (PSMs) --- p.36 / Chapter 1.3 --- Evolution of MRSA --- p.36 / Chapter 1.4 --- Epidemiology of MRSA --- p.38 / Chapter 1.4.1 --- Epidemiology of MRSA worldwide --- p.38 / Chapter 1.4.1.1 --- Epidemiology of HA-MRSA worldwide --- p.38 / Chapter 1.4.1.2 --- Epidemiology of CA-MRSA worldwide --- p.39 / Chapter 1.4.2 --- Epidemiology of MRSA in Hong Kong --- p.40 / Chapter 1.5 --- Clinical significance of MRSA --- p.41 / Chapter 1.6 --- Antibiotics --- p.43 / Chapter 1.6.1 --- Beta-lactams --- p.43 / Chapter 1.6.2 --- Fluoroquinolone --- p.44 / Chapter 1.6.3 --- Linezolid --- p.45 / Chapter 1.6.4 --- Glycopeptides --- p.45 / Chapter 1.6.5 --- Aminoglycosides --- p.46 / Chapter 1.6.6 --- Fusidic acid --- p.46 / Chapter 1.6.7 --- Clindamycin --- p.47 / Chapter 1.7 --- Hypothesis --- p.47 / Chapter Chapter 2 --- Methods and Materials --- p.50 / Chapter 2.1 --- Bacterial isolate --- p.50 / Chapter 2.2 --- Effect of subinhibitory antibiotics on the expression of mRNA in MRSA in vitro --- p.53 / Chapter 2.2.1 --- Collection of bacterial fraction --- p.53 / Chapter 2.2.2 --- RNA extraction and DNA digestion --- p.53 / Chapter 2.2.3 --- Reverse transcription for cDNA synthesis --- p.54 / Chapter 2.2.4 --- Quantitative real-time PCR (qPCR) analysis (pvl and psma\-A expression) --- p.55 / Chapter 2.2.5 --- Preparation of standard controls for quantification of DNA copy number in qPCR reactions... --- p.58 / Chapter 2.3 --- Effect of subinhibitory concentration of antibiotics on MRSA pneumonia in a murine model --- p.60 / Chapter 2.4 --- Statistical Analysis --- p.62 / Chapter Chapter 3 --- Results --- p.63 / Chapter 3.1 --- Effect of subinhibitory antibiotics on the expression ofmRNA in MRSA in vitro --- p.63 / Chapter 3.2 --- Effect of subinhibitory concentration of antibiotics on MRSA pneumonia in a murine model --- p.74 / Chapter Chapter 4 --- Discussion --- p.80 / Chapter 4.1 --- Effect of subinhibitory antibiotics on the expression of mRNA in MRSA in vitro --- p.81 / Chapter 4.2 --- Effect of subinhibitory concentration of antibiotics on MRSA pneumonia in a murine model --- p.87 / Chapter 4.3 --- Correlation of effects of subinhibitory antibiotics on the expression ofmRNA in MRSA in vitro and on MRSA pneumonia in a murine model --- p.91 / Chapter 4.4 --- Limitations of Study --- p.95 / Chapter 4.5 --- Future Work --- p.95 / Chapter Chapter 5 --- Conclusions --- p.97 / References --- p.99 / Chapter Appendix I- --- Materials and Reagents --- p.109 / Chapter Appendix II- --- Average and standard deviation of the copy number ratio (pvl or psmal-4 copy number/JdiS1 copy number) --- p.111 / Chapter Appendix III- --- In-vivo experimental data for infected control group and seven antibiotic groups --- p.116
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_327466 |
| Date | January 2011 |
| Contributors | Wong, Kai Yi., Chinese University of Hong Kong Graduate School. Division of Microbiology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, 122 leaves : col. ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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