The Receptor for Advanced Glycation End Products (RAGE) contains a single transmembrane helix, a small cytosolic domain, and an extracellular region (sRAGE) composed of three Ig-like domains (V, C1, C2). RAGE has been implicated in complications arising from diabetes and chronic inflammation and is widely thought to be a therapeutic target. In this dissertation, production of purified sRAGE and the five single and tandem domain constructs enabled biophysical and structural characterization. The results, including an x-ray crystal structure of VC1, show that the V and C1 domains form an integrated structural unit. In contrast, C2 is attached to VC1 by a flexible linker and is fully independent. Studies of the interaction with a known RAGE ligand, Ca2+-S100B, revealed a major contribution from the V domain but clearly defined contributions to binding from the C1 domain. The implications of these results are discussed with respect to models for sRAGE quaternary structure and RAGE signaling.
| Identifer | oai:union.ndltd.org:VANDERBILT/oai:VANDERBILTETD:etd-05302007-114804 |
| Date | 30 May 2007 |
| Creators | Dattilo, Brian Matthew |
| Contributors | Walter J. Chazin, Charles R. Sanders, II, Martin Egli, Billy G. Hudson |
| Publisher | VANDERBILT |
| Source Sets | Vanderbilt University Theses |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.library.vanderbilt.edu/available/etd-05302007-114804/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Vanderbilt University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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